Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3028 and GFF3029 are separated by 275 nucleotides GFF3029 and GFF3030 are separated by 100 nucleotides
GFF3028 - Transketolase (EC 2.2.1.1), at 41,527 to 43,518
GFF3028
GFF3029 - Putative metalloprotease yggG (EC 3.4.24.-), at 43,794 to 44,552
GFF3029
GFF3030 - Agmatinase (EC 3.5.3.11), at 44,653 to 45,573
GFF3030
Position (kb) 43
44
45 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 42.974 kb on + strand, within GFF3028 at 43.071 kb on - strand, within GFF3028 at 43.090 kb on - strand, within GFF3028 at 43.162 kb on - strand, within GFF3028 at 43.206 kb on - strand, within GFF3028 at 43.241 kb on - strand, within GFF3028 at 43.241 kb on - strand, within GFF3028 at 43.241 kb on - strand, within GFF3028 at 43.305 kb on - strand, within GFF3028 at 43.305 kb on - strand, within GFF3028 at 43.488 kb on + strand at 43.506 kb on + strand at 43.609 kb on + strand at 43.609 kb on + strand at 43.678 kb on + strand at 43.679 kb on - strand at 43.679 kb on - strand at 43.721 kb on + strand at 43.722 kb on - strand at 43.811 kb on - strand at 43.865 kb on + strand at 43.865 kb on + strand at 43.866 kb on - strand at 43.907 kb on + strand, within GFF3029 at 43.908 kb on - strand, within GFF3029 at 43.999 kb on - strand, within GFF3029 at 44.064 kb on + strand, within GFF3029 at 44.064 kb on + strand, within GFF3029 at 44.065 kb on - strand, within GFF3029 at 44.065 kb on - strand, within GFF3029 at 44.067 kb on - strand, within GFF3029 at 44.118 kb on - strand, within GFF3029 at 44.212 kb on + strand, within GFF3029 at 44.377 kb on - strand, within GFF3029 at 44.616 kb on + strand at 44.679 kb on + strand at 44.681 kb on + strand at 44.681 kb on + strand at 44.692 kb on + strand at 44.738 kb on + strand at 44.739 kb on - strand at 44.897 kb on + strand, within GFF3030 at 44.941 kb on - strand, within GFF3030 at 45.071 kb on + strand, within GFF3030 at 45.110 kb on + strand, within GFF3030 at 45.219 kb on + strand, within GFF3030 at 45.220 kb on - strand, within GFF3030 at 45.297 kb on - strand, within GFF3030 at 45.297 kb on - strand, within GFF3030
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 42,974 + GFF3028 0.73 -0.9 43,071 - GFF3028 0.78 +0.2 43,090 - GFF3028 0.78 -0.4 43,162 - GFF3028 0.82 -0.1 43,206 - GFF3028 0.84 -0.7 43,241 - GFF3028 0.86 +0.1 43,241 - GFF3028 0.86 +0.0 43,241 - GFF3028 0.86 -0.0 43,305 - GFF3028 0.89 -0.7 43,305 - GFF3028 0.89 +1.6 43,488 + +1.0 43,506 + +3.1 43,609 + -0.2 43,609 + -0.0 43,678 + +0.5 43,679 - +0.3 43,679 - -0.2 43,721 + +0.2 43,722 - -2.0 43,811 - +0.1 43,865 + -0.6 43,865 + +0.6 43,866 - +0.1 43,907 + GFF3029 0.15 -0.1 43,908 - GFF3029 0.15 -0.1 43,999 - GFF3029 0.27 -0.3 44,064 + GFF3029 0.36 +0.1 44,064 + GFF3029 0.36 -0.7 44,065 - GFF3029 0.36 -0.6 44,065 - GFF3029 0.36 +0.4 44,067 - GFF3029 0.36 -0.5 44,118 - GFF3029 0.43 -0.1 44,212 + GFF3029 0.55 +0.4 44,377 - GFF3029 0.77 -0.4 44,616 + -0.1 44,679 + +0.0 44,681 + -0.2 44,681 + +1.5 44,692 + +1.1 44,738 + -0.4 44,739 - +0.8 44,897 + GFF3030 0.26 -0.5 44,941 - GFF3030 0.31 -0.0 45,071 + GFF3030 0.45 +0.1 45,110 + GFF3030 0.50 -0.5 45,219 + GFF3030 0.61 -0.3 45,220 - GFF3030 0.62 -0.5 45,297 - GFF3030 0.70 +1.0 45,297 - GFF3030 0.70 +0.0
Or see this region's nucleotide sequence