Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3017 and GFF3018 are separated by 93 nucleotides GFF3018 and GFF3019 are separated by 199 nucleotides GFF3019 and GFF3020 are separated by 226 nucleotides
GFF3017 - Protein of unknown function DUF541, at 31,883 to 32,629
GFF3017
GFF3018 - Arginine exporter protein ArgO, at 32,723 to 33,358
GFF3018
GFF3019 - Protein involved in stability of MscS mechanosensitive channel, at 33,558 to 34,418
GFF3019
GFF3020 - Fructose-bisphosphate aldolase class II (EC 4.1.2.13), at 34,645 to 35,724
GFF3020
Position (kb) 33
34
35 Strain fitness (log2 ratio) -1
0
1
2 at 32.663 kb on + strand at 32.664 kb on - strand at 32.665 kb on + strand at 32.665 kb on + strand at 32.665 kb on + strand at 32.666 kb on - strand at 32.724 kb on + strand at 32.725 kb on - strand at 32.725 kb on - strand at 32.743 kb on + strand at 32.813 kb on - strand, within GFF3018 at 32.975 kb on - strand, within GFF3018 at 32.983 kb on - strand, within GFF3018 at 32.983 kb on - strand, within GFF3018 at 33.050 kb on - strand, within GFF3018 at 33.117 kb on - strand, within GFF3018 at 33.242 kb on + strand, within GFF3018 at 33.500 kb on + strand at 33.620 kb on + strand at 33.624 kb on - strand at 33.648 kb on - strand, within GFF3019 at 33.827 kb on + strand, within GFF3019 at 33.896 kb on + strand, within GFF3019 at 34.016 kb on - strand, within GFF3019 at 34.178 kb on + strand, within GFF3019 at 34.239 kb on - strand, within GFF3019 at 34.263 kb on + strand, within GFF3019 at 34.307 kb on + strand, within GFF3019 at 34.310 kb on + strand, within GFF3019 at 34.339 kb on + strand at 34.339 kb on + strand at 34.339 kb on + strand at 34.340 kb on - strand at 34.641 kb on + strand at 34.647 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 32,663 + -0.4 32,664 - -0.4 32,665 + -0.1 32,665 + +0.2 32,665 + -0.3 32,666 - -0.4 32,724 + +0.5 32,725 - +0.2 32,725 - -1.3 32,743 + +0.2 32,813 - GFF3018 0.14 +0.3 32,975 - GFF3018 0.40 +0.4 32,983 - GFF3018 0.41 -0.6 32,983 - GFF3018 0.41 +0.3 33,050 - GFF3018 0.51 +0.2 33,117 - GFF3018 0.62 +2.0 33,242 + GFF3018 0.82 +0.5 33,500 + -0.2 33,620 + +0.3 33,624 - +0.3 33,648 - GFF3019 0.10 -0.1 33,827 + GFF3019 0.31 +0.1 33,896 + GFF3019 0.39 +0.4 34,016 - GFF3019 0.53 -0.2 34,178 + GFF3019 0.72 +1.3 34,239 - GFF3019 0.79 +0.9 34,263 + GFF3019 0.82 +1.2 34,307 + GFF3019 0.87 -0.3 34,310 + GFF3019 0.87 -0.2 34,339 + +0.4 34,339 + +0.4 34,339 + -0.6 34,340 - -0.4 34,641 + -1.1 34,647 - +0.0
Or see this region's nucleotide sequence