Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF298 and GFF299 are separated by 3 nucleotides GFF299 and GFF300 are separated by 1 nucleotides GFF300 and GFF301 are separated by 324 nucleotides
GFF298 - Acetolactate synthase large subunit (EC 2.2.1.6), at 15,473 to 17,161
GFF298
GFF299 - Acetolactate synthase small subunit (EC 2.2.1.6), at 17,165 to 17,455
GFF299
GFF300 - Deoxyribose operon repressor, DeoR family, at 17,457 to 18,242
GFF300
GFF301 - Ribokinase (EC 2.7.1.15), at 18,567 to 19,487
GFF301
Position (kb) 17
18
19 Strain fitness (log2 ratio) -1
0
1 at 16.462 kb on - strand, within GFF298 at 16.644 kb on + strand, within GFF298 at 16.823 kb on - strand, within GFF298 at 16.903 kb on - strand, within GFF298 at 16.925 kb on + strand, within GFF298 at 16.928 kb on - strand, within GFF298 at 17.050 kb on + strand at 17.458 kb on + strand at 17.458 kb on + strand at 17.519 kb on - strand at 17.543 kb on + strand, within GFF300 at 17.724 kb on - strand, within GFF300 at 17.776 kb on + strand, within GFF300 at 17.832 kb on - strand, within GFF300 at 18.035 kb on + strand, within GFF300 at 18.056 kb on + strand, within GFF300 at 18.071 kb on + strand, within GFF300 at 18.268 kb on + strand at 18.323 kb on + strand at 18.324 kb on - strand at 18.325 kb on + strand at 18.325 kb on + strand at 18.326 kb on - strand at 18.605 kb on + strand at 18.612 kb on + strand at 18.613 kb on - strand at 18.755 kb on + strand, within GFF301 at 18.810 kb on + strand, within GFF301 at 18.810 kb on + strand, within GFF301 at 18.811 kb on - strand, within GFF301 at 18.811 kb on - strand, within GFF301 at 18.811 kb on - strand, within GFF301 at 18.823 kb on + strand, within GFF301 at 19.079 kb on + strand, within GFF301 at 19.086 kb on + strand, within GFF301 at 19.087 kb on - strand, within GFF301 at 19.087 kb on - strand, within GFF301 at 19.157 kb on + strand, within GFF301 at 19.162 kb on - strand, within GFF301 at 19.217 kb on + strand, within GFF301 at 19.217 kb on + strand, within GFF301
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 16,462 - GFF298 0.59 -0.0 16,644 + GFF298 0.69 +1.3 16,823 - GFF298 0.80 -0.1 16,903 - GFF298 0.85 -0.0 16,925 + GFF298 0.86 -0.8 16,928 - GFF298 0.86 -0.8 17,050 + -1.0 17,458 + -1.0 17,458 + +0.0 17,519 - +0.8 17,543 + GFF300 0.11 +0.2 17,724 - GFF300 0.34 +0.3 17,776 + GFF300 0.41 -1.2 17,832 - GFF300 0.48 +0.7 18,035 + GFF300 0.74 -0.0 18,056 + GFF300 0.76 -1.0 18,071 + GFF300 0.78 -1.1 18,268 + -0.1 18,323 + +0.2 18,324 - +0.1 18,325 + -0.2 18,325 + -0.0 18,326 - -1.2 18,605 + +0.2 18,612 + -0.1 18,613 - -0.4 18,755 + GFF301 0.20 +0.1 18,810 + GFF301 0.26 +0.1 18,810 + GFF301 0.26 -0.9 18,811 - GFF301 0.26 -0.2 18,811 - GFF301 0.26 +0.4 18,811 - GFF301 0.26 -0.1 18,823 + GFF301 0.28 -1.1 19,079 + GFF301 0.56 +0.3 19,086 + GFF301 0.56 -1.0 19,087 - GFF301 0.56 -0.5 19,087 - GFF301 0.56 -0.5 19,157 + GFF301 0.64 -0.9 19,162 - GFF301 0.65 +0.8 19,217 + GFF301 0.71 +1.4 19,217 + GFF301 0.71 -0.2
Or see this region's nucleotide sequence