Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2979 and GFF2980 overlap by 4 nucleotides GFF2980 and GFF2981 are separated by 549 nucleotides GFF2981 and GFF2982 overlap by 45 nucleotides GFF2982 and GFF2983 are separated by 18 nucleotides
GFF2979 - VapC toxin protein, at 290 to 691
GFF2979
GFF2980 - VapB protein (antitoxin to VapC), at 688 to 870
GFF2980
GFF2981 - FIG01047567: hypothetical protein, at 1,420 to 1,533
GFF2981
GFF2982 - hypothetical protein, at 1,489 to 1,605
GFF2982
GFF2983 - probable lipoprotein YPO2292, at 1,624 to 2,424
GFF2983
Position (kb) 0
1 Strain fitness (log2 ratio) -1
0
1 at 0.455 kb on + strand, within GFF2979 at 0.455 kb on + strand, within GFF2979 at 0.455 kb on + strand, within GFF2979 at 0.456 kb on - strand, within GFF2979 at 0.456 kb on - strand, within GFF2979 at 0.500 kb on - strand, within GFF2979 at 0.500 kb on - strand, within GFF2979 at 0.523 kb on + strand, within GFF2979 at 0.555 kb on + strand, within GFF2979 at 0.602 kb on - strand, within GFF2979 at 0.602 kb on - strand, within GFF2979 at 0.603 kb on - strand, within GFF2979 at 0.656 kb on - strand at 0.662 kb on - strand at 0.662 kb on - strand at 0.915 kb on + strand at 0.972 kb on - strand at 0.972 kb on - strand at 1.229 kb on + strand at 1.229 kb on + strand at 1.234 kb on + strand at 1.235 kb on - strand at 1.410 kb on - strand at 1.423 kb on + strand at 1.423 kb on + strand at 1.423 kb on + strand at 1.424 kb on - strand at 1.538 kb on + strand, within GFF2982 at 1.623 kb on - strand at 1.623 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 455 + GFF2979 0.41 +0.2 455 + GFF2979 0.41 +0.0 455 + GFF2979 0.41 +0.1 456 - GFF2979 0.41 +0.1 456 - GFF2979 0.41 +0.5 500 - GFF2979 0.52 +1.3 500 - GFF2979 0.52 +0.1 523 + GFF2979 0.58 -0.2 555 + GFF2979 0.66 +0.3 602 - GFF2979 0.78 +0.0 602 - GFF2979 0.78 -0.0 603 - GFF2979 0.78 +0.4 656 - +0.3 662 - -0.6 662 - -0.3 915 + +0.0 972 - -0.2 972 - +0.5 1,229 + -0.1 1,229 + +0.1 1,234 + -1.0 1,235 - -0.5 1,410 - +0.4 1,423 + +0.6 1,423 + +0.8 1,423 + -0.2 1,424 - +0.3 1,538 + GFF2982 0.42 +0.3 1,623 - +0.0 1,623 - +0.7
Or see this region's nucleotide sequence