Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2956 and GFF2957 are separated by 9 nucleotides GFF2957 and GFF2958 are separated by 206 nucleotides
GFF2956 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 35,999 to 40,459
GFF2956
GFF2957 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 40,469 to 41,887
GFF2957
GFF2958 - Putative uncharacterized protein YhcG, at 42,094 to 43,197
GFF2958
Position (kb) 40
41
42 Strain fitness (log2 ratio) -1
0
1 at 39.610 kb on + strand, within GFF2956 at 39.750 kb on - strand, within GFF2956 at 39.861 kb on + strand, within GFF2956 at 39.861 kb on + strand, within GFF2956 at 39.861 kb on + strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.863 kb on + strand, within GFF2956 at 39.864 kb on - strand, within GFF2956 at 39.887 kb on + strand, within GFF2956 at 39.888 kb on - strand, within GFF2956 at 39.990 kb on - strand, within GFF2956 at 40.103 kb on + strand at 40.103 kb on + strand at 40.104 kb on - strand at 40.181 kb on + strand at 40.233 kb on + strand at 40.234 kb on - strand at 40.240 kb on - strand at 40.240 kb on - strand at 40.309 kb on + strand at 40.420 kb on + strand at 40.654 kb on + strand, within GFF2957 at 40.654 kb on + strand, within GFF2957 at 40.654 kb on + strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.658 kb on + strand, within GFF2957 at 40.658 kb on + strand, within GFF2957 at 40.658 kb on + strand, within GFF2957 at 40.659 kb on - strand, within GFF2957 at 40.659 kb on - strand, within GFF2957 at 40.659 kb on - strand, within GFF2957 at 41.113 kb on + strand, within GFF2957 at 41.136 kb on + strand, within GFF2957 at 41.173 kb on - strand, within GFF2957 at 41.311 kb on + strand, within GFF2957 at 41.312 kb on + strand, within GFF2957 at 41.312 kb on + strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.410 kb on - strand, within GFF2957 at 41.410 kb on - strand, within GFF2957 at 41.500 kb on + strand, within GFF2957 at 41.501 kb on - strand, within GFF2957 at 41.756 kb on - strand at 41.873 kb on + strand at 41.951 kb on + strand at 41.995 kb on + strand at 41.995 kb on + strand at 41.995 kb on + strand at 41.996 kb on - strand at 41.996 kb on - strand at 41.996 kb on - strand at 42.131 kb on - strand at 42.475 kb on + strand, within GFF2958 at 42.827 kb on + strand, within GFF2958 at 42.827 kb on + strand, within GFF2958 at 42.827 kb on + strand, within GFF2958
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 39,610 + GFF2956 0.81 -0.4 39,750 - GFF2956 0.84 +0.6 39,861 + GFF2956 0.87 +0.2 39,861 + GFF2956 0.87 +0.2 39,861 + GFF2956 0.87 +0.1 39,862 - GFF2956 0.87 -0.2 39,862 - GFF2956 0.87 +0.8 39,862 - GFF2956 0.87 -1.0 39,862 - GFF2956 0.87 +0.1 39,863 + GFF2956 0.87 +0.3 39,864 - GFF2956 0.87 +0.3 39,887 + GFF2956 0.87 +0.4 39,888 - GFF2956 0.87 +0.0 39,990 - GFF2956 0.89 +0.0 40,103 + -0.0 40,103 + -0.2 40,104 - +0.1 40,181 + +0.3 40,233 + -0.1 40,234 - +0.1 40,240 - +0.1 40,240 - -0.7 40,309 + -0.7 40,420 + +0.2 40,654 + GFF2957 0.13 +0.5 40,654 + GFF2957 0.13 +0.3 40,654 + GFF2957 0.13 +0.1 40,655 - GFF2957 0.13 +0.1 40,655 - GFF2957 0.13 +0.4 40,655 - GFF2957 0.13 +0.6 40,655 - GFF2957 0.13 -0.2 40,658 + GFF2957 0.13 +0.8 40,658 + GFF2957 0.13 +0.7 40,658 + GFF2957 0.13 -0.3 40,659 - GFF2957 0.13 -0.1 40,659 - GFF2957 0.13 +0.4 40,659 - GFF2957 0.13 +0.2 41,113 + GFF2957 0.45 -0.5 41,136 + GFF2957 0.47 -0.1 41,173 - GFF2957 0.50 +0.4 41,311 + GFF2957 0.59 -0.4 41,312 + GFF2957 0.59 +0.3 41,312 + GFF2957 0.59 +0.3 41,313 - GFF2957 0.59 -0.1 41,313 - GFF2957 0.59 +0.4 41,313 - GFF2957 0.59 +0.0 41,410 - GFF2957 0.66 +0.4 41,410 - GFF2957 0.66 +0.1 41,500 + GFF2957 0.73 +0.9 41,501 - GFF2957 0.73 -0.3 41,756 - +0.7 41,873 + +0.1 41,951 + +0.0 41,995 + +0.1 41,995 + +0.0 41,995 + -0.4 41,996 - -0.2 41,996 - +0.8 41,996 - +0.5 42,131 - -0.5 42,475 + GFF2958 0.35 +0.1 42,827 + GFF2958 0.66 +1.4 42,827 + GFF2958 0.66 -1.0 42,827 + GFF2958 0.66 +0.3
Or see this region's nucleotide sequence