Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF294 and GFF295 are separated by 225 nucleotides
GFF294 - D-serine permease DsdX, at 8,643 to 9,980
GFF294
GFF295 - D-serine dehydratase transcriptional activator, at 10,206 to 11,129
GFF295
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-1
0
1 at 9.387 kb on + strand, within GFF294 at 9.432 kb on + strand, within GFF294 at 9.433 kb on - strand, within GFF294 at 9.439 kb on + strand, within GFF294 at 9.581 kb on + strand, within GFF294 at 9.924 kb on + strand at 9.925 kb on - strand at 9.981 kb on - strand at 9.990 kb on - strand at 10.237 kb on + strand at 10.266 kb on + strand at 10.268 kb on + strand at 10.353 kb on - strand, within GFF295 at 10.527 kb on + strand, within GFF295 at 10.581 kb on - strand, within GFF295 at 10.629 kb on - strand, within GFF295 at 10.664 kb on + strand, within GFF295 at 10.668 kb on + strand, within GFF295 at 10.668 kb on + strand, within GFF295 at 10.668 kb on + strand, within GFF295 at 10.669 kb on - strand, within GFF295 at 10.807 kb on + strand, within GFF295 at 10.830 kb on - strand, within GFF295 at 11.052 kb on + strand at 11.053 kb on - strand at 11.103 kb on - strand at 11.135 kb on + strand at 11.170 kb on + strand at 11.175 kb on + strand at 11.179 kb on + strand at 11.180 kb on - strand at 11.231 kb on + strand at 11.473 kb on + strand at 11.474 kb on - strand at 11.487 kb on + strand at 11.487 kb on + strand at 11.491 kb on - strand at 11.508 kb on - strand at 11.530 kb on - strand at 11.531 kb on + strand at 11.657 kb on + strand at 11.658 kb on - strand at 11.658 kb on - strand at 11.667 kb on - strand at 11.686 kb on + strand at 11.686 kb on + strand at 11.686 kb on + strand at 11.686 kb on + strand at 11.687 kb on - strand at 11.689 kb on - strand at 11.787 kb on - strand at 11.811 kb on + strand at 11.811 kb on + strand at 11.811 kb on + strand at 11.811 kb on + strand at 11.864 kb on + strand at 11.865 kb on - strand at 11.945 kb on + strand at 12.079 kb on + strand at 12.099 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 9,387 + GFF294 0.56 -0.4 9,432 + GFF294 0.59 +1.0 9,433 - GFF294 0.59 -0.2 9,439 + GFF294 0.59 -0.2 9,581 + GFF294 0.70 +0.9 9,924 + -0.4 9,925 - -0.4 9,981 - +0.6 9,990 - +0.4 10,237 + -0.1 10,266 + +0.1 10,268 + -0.1 10,353 - GFF295 0.16 -0.8 10,527 + GFF295 0.35 -0.1 10,581 - GFF295 0.41 -0.1 10,629 - GFF295 0.46 +0.1 10,664 + GFF295 0.50 -0.9 10,668 + GFF295 0.50 -1.4 10,668 + GFF295 0.50 +0.0 10,668 + GFF295 0.50 -0.1 10,669 - GFF295 0.50 -0.8 10,807 + GFF295 0.65 +0.4 10,830 - GFF295 0.68 -0.4 11,052 + +0.0 11,053 - -0.5 11,103 - -0.2 11,135 + -0.1 11,170 + +0.2 11,175 + -0.5 11,179 + -0.8 11,180 - +0.0 11,231 + -0.0 11,473 + -0.3 11,474 - -0.1 11,487 + -0.4 11,487 + -0.4 11,491 - +0.5 11,508 - -0.1 11,530 - +0.3 11,531 + +0.0 11,657 + +0.0 11,658 - +0.8 11,658 - +0.5 11,667 - +0.2 11,686 + +0.7 11,686 + +0.0 11,686 + +0.4 11,686 + +0.4 11,687 - -0.2 11,689 - +0.1 11,787 - +0.4 11,811 + -0.2 11,811 + -0.5 11,811 + +0.0 11,811 + -0.1 11,864 + -0.1 11,865 - -0.1 11,945 + -0.1 12,079 + -0.7 12,099 + -0.3
Or see this region's nucleotide sequence