Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2757 and GFF2758 are separated by 35 nucleotides GFF2758 and GFF2759 are separated by 41 nucleotides GFF2759 and GFF2760 overlap by 1 nucleotides GFF2760 and GFF2761 overlap by 4 nucleotides
GFF2757 - Putative secreted protein, at 278,995 to 280,524
GFF2757
GFF2758 - FIG01045187: hypothetical protein, at 280,560 to 280,724
GFF2758
GFF2759 - Respiratory nitrate reductase gamma chain (EC 1.7.99.4), at 280,766 to 281,443
GFF2759
GFF2760 - Respiratory nitrate reductase delta chain (EC 1.7.99.4), at 281,443 to 282,153
GFF2760
GFF2761 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 282,150 to 283,685
GFF2761
Position (kb) 281
282
283 Strain fitness (log2 ratio) -1
0
1
2 at 280.697 kb on + strand, within GFF2758 at 280.807 kb on - strand at 280.828 kb on + strand at 280.898 kb on - strand, within GFF2759 at 280.904 kb on + strand, within GFF2759 at 281.124 kb on - strand, within GFF2759 at 281.214 kb on + strand, within GFF2759 at 281.410 kb on - strand at 281.414 kb on - strand at 281.444 kb on + strand at 281.444 kb on + strand at 281.914 kb on + strand, within GFF2760 at 281.915 kb on - strand, within GFF2760 at 281.915 kb on - strand, within GFF2760 at 281.915 kb on - strand, within GFF2760 at 281.981 kb on - strand, within GFF2760 at 282.334 kb on + strand, within GFF2761 at 282.338 kb on + strand, within GFF2761 at 282.445 kb on + strand, within GFF2761 at 282.523 kb on + strand, within GFF2761 at 282.625 kb on + strand, within GFF2761 at 282.874 kb on - strand, within GFF2761 at 283.084 kb on + strand, within GFF2761
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 280,697 + GFF2758 0.83 +0.4 280,807 - +0.2 280,828 + +2.0 280,898 - GFF2759 0.19 +0.1 280,904 + GFF2759 0.20 +0.1 281,124 - GFF2759 0.53 +0.8 281,214 + GFF2759 0.66 +0.6 281,410 - +0.1 281,414 - +0.1 281,444 + +0.5 281,444 + +0.3 281,914 + GFF2760 0.66 +0.7 281,915 - GFF2760 0.66 -0.0 281,915 - GFF2760 0.66 +0.8 281,915 - GFF2760 0.66 +0.1 281,981 - GFF2760 0.76 +1.3 282,334 + GFF2761 0.12 +0.2 282,338 + GFF2761 0.12 +0.1 282,445 + GFF2761 0.19 -0.1 282,523 + GFF2761 0.24 +0.3 282,625 + GFF2761 0.31 +0.1 282,874 - GFF2761 0.47 +0.6 283,084 + GFF2761 0.61 -0.5
Or see this region's nucleotide sequence