Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2714 and GFF2715 are separated by 402 nucleotides GFF2715 and GFF2716 are separated by 41 nucleotides GFF2716 and GFF2717 are separated by 237 nucleotides GFF2717 and GFF2718 overlap by 4 nucleotides
GFF2714 - DNA topoisomerase I (EC 5.99.1.2), at 235,410 to 238,007
GFF2714
GFF2715 - FIG00948954: hypothetical protein YciN, at 238,410 to 238,661
GFF2715
GFF2716 - Possible protease sohB (EC 3.4.21.-), at 238,703 to 239,749
GFF2716
GFF2717 - YciK-like oxidoreductase, at 239,987 to 240,748
GFF2717
GFF2718 - Cob(I)alamin adenosyltransferase (EC 2.5.1.17), at 240,745 to 241,335
GFF2718
Position (kb) 238
239
240 Strain fitness (log2 ratio) -2
-1
0
1 at 238.018 kb on - strand at 238.086 kb on - strand at 238.086 kb on - strand at 238.086 kb on - strand at 238.088 kb on - strand at 238.088 kb on - strand at 238.288 kb on + strand at 238.289 kb on - strand at 238.470 kb on - strand, within GFF2715 at 238.499 kb on + strand, within GFF2715 at 238.604 kb on + strand, within GFF2715 at 238.808 kb on - strand, within GFF2716 at 238.810 kb on - strand, within GFF2716 at 238.871 kb on - strand, within GFF2716 at 239.092 kb on + strand, within GFF2716 at 239.105 kb on - strand, within GFF2716 at 239.164 kb on + strand, within GFF2716 at 239.165 kb on - strand, within GFF2716 at 239.209 kb on + strand, within GFF2716 at 239.209 kb on + strand, within GFF2716 at 239.209 kb on + strand, within GFF2716 at 239.211 kb on + strand, within GFF2716 at 239.436 kb on - strand, within GFF2716 at 239.436 kb on - strand, within GFF2716 at 239.436 kb on - strand, within GFF2716 at 239.516 kb on - strand, within GFF2716 at 239.656 kb on + strand at 239.765 kb on + strand at 239.791 kb on - strand at 239.982 kb on + strand at 240.028 kb on + strand at 240.081 kb on - strand, within GFF2717 at 240.255 kb on - strand, within GFF2717 at 240.433 kb on + strand, within GFF2717 at 240.480 kb on - strand, within GFF2717
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 238,018 - -2.2 238,086 - -0.8 238,086 - -0.3 238,086 - -0.6 238,088 - -0.2 238,088 - +0.0 238,288 + -0.4 238,289 - +0.7 238,470 - GFF2715 0.24 +0.1 238,499 + GFF2715 0.35 +0.0 238,604 + GFF2715 0.77 +0.4 238,808 - GFF2716 0.10 -0.3 238,810 - GFF2716 0.10 -0.3 238,871 - GFF2716 0.16 +1.1 239,092 + GFF2716 0.37 +0.1 239,105 - GFF2716 0.38 +0.4 239,164 + GFF2716 0.44 +0.6 239,165 - GFF2716 0.44 -0.4 239,209 + GFF2716 0.48 +0.1 239,209 + GFF2716 0.48 +1.5 239,209 + GFF2716 0.48 +0.7 239,211 + GFF2716 0.49 +0.0 239,436 - GFF2716 0.70 +0.9 239,436 - GFF2716 0.70 +0.2 239,436 - GFF2716 0.70 -0.7 239,516 - GFF2716 0.78 +0.4 239,656 + -0.3 239,765 + +0.2 239,791 - +0.1 239,982 + -0.6 240,028 + -0.7 240,081 - GFF2717 0.12 +0.5 240,255 - GFF2717 0.35 -0.0 240,433 + GFF2717 0.59 +0.2 240,480 - GFF2717 0.65 -0.1
Or see this region's nucleotide sequence