Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2698 and GFF2699 are separated by 252 nucleotides GFF2699 and GFF2700 are separated by 121 nucleotides GFF2700 and GFF2701 are separated by 86 nucleotides
GFF2698 - Exoribonuclease II (EC 3.1.13.1), at 219,964 to 221,898
GFF2698
GFF2699 - FIG00637999: hypothetical protein, at 222,151 to 224,142
GFF2699
GFF2700 - FIG01045186: hypothetical protein, at 224,264 to 224,443
GFF2700
GFF2701 - Transcriptional regulatory protein YciT, at 224,530 to 225,282
GFF2701
Position (kb) 222
223
224
225 Strain fitness (log2 ratio) -2
-1
0
1
2 at 221.207 kb on + strand, within GFF2698 at 221.211 kb on + strand, within GFF2698 at 221.463 kb on + strand, within GFF2698 at 221.541 kb on + strand, within GFF2698 at 221.542 kb on - strand, within GFF2698 at 221.542 kb on - strand, within GFF2698 at 221.542 kb on - strand, within GFF2698 at 221.609 kb on + strand, within GFF2698 at 221.611 kb on + strand, within GFF2698 at 221.611 kb on + strand, within GFF2698 at 221.612 kb on - strand, within GFF2698 at 221.612 kb on - strand, within GFF2698 at 221.612 kb on - strand, within GFF2698 at 221.612 kb on - strand, within GFF2698 at 221.646 kb on + strand, within GFF2698 at 221.688 kb on + strand, within GFF2698 at 221.787 kb on + strand at 221.787 kb on + strand at 222.056 kb on + strand at 222.062 kb on + strand at 222.104 kb on + strand at 222.156 kb on - strand at 222.241 kb on + strand at 222.242 kb on - strand at 222.287 kb on + strand at 222.396 kb on + strand, within GFF2699 at 222.396 kb on + strand, within GFF2699 at 222.500 kb on + strand, within GFF2699 at 222.664 kb on + strand, within GFF2699 at 223.294 kb on + strand, within GFF2699 at 223.295 kb on - strand, within GFF2699 at 223.419 kb on + strand, within GFF2699 at 223.419 kb on + strand, within GFF2699 at 223.458 kb on - strand, within GFF2699 at 223.478 kb on + strand, within GFF2699 at 223.478 kb on + strand, within GFF2699 at 223.532 kb on - strand, within GFF2699 at 223.586 kb on + strand, within GFF2699 at 223.690 kb on - strand, within GFF2699 at 223.923 kb on + strand, within GFF2699 at 224.006 kb on + strand at 224.110 kb on + strand at 224.183 kb on + strand at 224.184 kb on - strand at 224.184 kb on - strand at 224.354 kb on - strand, within GFF2700 at 224.529 kb on + strand at 224.529 kb on + strand at 224.666 kb on - strand, within GFF2701 at 224.666 kb on - strand, within GFF2701 at 224.670 kb on - strand, within GFF2701 at 224.745 kb on - strand, within GFF2701 at 224.884 kb on + strand, within GFF2701 at 224.966 kb on + strand, within GFF2701
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 221,207 + GFF2698 0.64 -0.8 221,211 + GFF2698 0.64 +0.1 221,463 + GFF2698 0.77 +1.0 221,541 + GFF2698 0.81 +1.5 221,542 - GFF2698 0.82 -1.0 221,542 - GFF2698 0.82 +0.1 221,542 - GFF2698 0.82 +2.1 221,609 + GFF2698 0.85 +0.8 221,611 + GFF2698 0.85 -2.5 221,611 + GFF2698 0.85 -0.1 221,612 - GFF2698 0.85 -1.3 221,612 - GFF2698 0.85 +1.0 221,612 - GFF2698 0.85 -0.5 221,612 - GFF2698 0.85 -2.6 221,646 + GFF2698 0.87 -0.5 221,688 + GFF2698 0.89 +0.0 221,787 + -1.4 221,787 + +0.7 222,056 + +0.0 222,062 + -0.0 222,104 + -0.1 222,156 - +0.4 222,241 + +0.6 222,242 - +0.5 222,287 + +0.0 222,396 + GFF2699 0.12 +0.8 222,396 + GFF2699 0.12 +0.9 222,500 + GFF2699 0.18 -0.4 222,664 + GFF2699 0.26 +0.6 223,294 + GFF2699 0.57 +0.4 223,295 - GFF2699 0.57 +1.2 223,419 + GFF2699 0.64 -0.4 223,419 + GFF2699 0.64 -0.1 223,458 - GFF2699 0.66 +0.2 223,478 + GFF2699 0.67 +0.2 223,478 + GFF2699 0.67 +0.5 223,532 - GFF2699 0.69 -0.3 223,586 + GFF2699 0.72 -0.8 223,690 - GFF2699 0.77 +0.1 223,923 + GFF2699 0.89 +0.4 224,006 + -0.0 224,110 + -0.4 224,183 + +0.3 224,184 - +0.0 224,184 - -0.4 224,354 - GFF2700 0.50 +0.2 224,529 + -1.2 224,529 + -0.6 224,666 - GFF2701 0.18 -0.4 224,666 - GFF2701 0.18 -0.3 224,670 - GFF2701 0.19 -0.8 224,745 - GFF2701 0.29 -1.0 224,884 + GFF2701 0.47 -1.0 224,966 + GFF2701 0.58 +0.6
Or see this region's nucleotide sequence