Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2680 and GFF2681 overlap by 4 nucleotides GFF2681 and GFF2682 are separated by 148 nucleotides GFF2682 and GFF2683 are separated by 75 nucleotides GFF2683 and GFF2684 are separated by 22 nucleotides
GFF2680 - Membrane protein YcjF, at 203,487 to 204,548
GFF2680
GFF2681 - Conserved protein YcjX with nucleoside triphosphate hydrolase domain, at 204,545 to 205,942
GFF2681
GFF2682 - Phage shock protein E precursor, at 206,091 to 206,405
GFF2682
GFF2683 - Phage shock protein D, at 206,481 to 206,699
GFF2683
GFF2684 - Phage shock protein C, at 206,722 to 207,081
GFF2684
Position (kb) 204
205
206 Strain fitness (log2 ratio) -2
-1
0
1
2 at 203.574 kb on - strand at 203.574 kb on - strand at 203.605 kb on - strand, within GFF2680 at 203.791 kb on + strand, within GFF2680 at 203.791 kb on + strand, within GFF2680 at 203.794 kb on + strand, within GFF2680 at 203.812 kb on + strand, within GFF2680 at 203.812 kb on + strand, within GFF2680 at 203.812 kb on + strand, within GFF2680 at 203.868 kb on - strand, within GFF2680 at 204.025 kb on + strand, within GFF2680 at 204.117 kb on - strand, within GFF2680 at 204.120 kb on + strand, within GFF2680 at 204.289 kb on - strand, within GFF2680 at 204.444 kb on - strand at 204.444 kb on - strand at 204.492 kb on - strand at 204.557 kb on - strand at 204.566 kb on + strand at 204.610 kb on + strand at 204.832 kb on + strand, within GFF2681 at 204.833 kb on - strand, within GFF2681 at 204.833 kb on - strand, within GFF2681 at 204.871 kb on + strand, within GFF2681 at 204.872 kb on - strand, within GFF2681 at 204.895 kb on + strand, within GFF2681 at 204.896 kb on - strand, within GFF2681 at 205.039 kb on + strand, within GFF2681 at 205.155 kb on + strand, within GFF2681 at 205.158 kb on + strand, within GFF2681 at 205.334 kb on - strand, within GFF2681 at 205.464 kb on - strand, within GFF2681 at 205.493 kb on + strand, within GFF2681 at 205.561 kb on - strand, within GFF2681 at 205.561 kb on - strand, within GFF2681 at 205.561 kb on - strand, within GFF2681 at 205.689 kb on - strand, within GFF2681 at 205.702 kb on - strand, within GFF2681 at 205.996 kb on - strand at 206.017 kb on + strand at 206.150 kb on + strand, within GFF2682 at 206.209 kb on + strand, within GFF2682 at 206.283 kb on - strand, within GFF2682 at 206.342 kb on - strand, within GFF2682 at 206.406 kb on - strand at 206.424 kb on - strand at 206.442 kb on + strand at 206.443 kb on - strand at 206.445 kb on - strand at 206.516 kb on + strand, within GFF2683 at 206.581 kb on - strand, within GFF2683 at 206.646 kb on + strand, within GFF2683 at 206.714 kb on + strand at 206.714 kb on + strand at 206.717 kb on - strand at 206.723 kb on + strand at 206.723 kb on + strand at 206.884 kb on - strand, within GFF2684 at 206.893 kb on + strand, within GFF2684 at 206.913 kb on - strand, within GFF2684 at 206.913 kb on - strand, within GFF2684
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 203,574 - +0.2 203,574 - -1.0 203,605 - GFF2680 0.11 +0.2 203,791 + GFF2680 0.29 -0.8 203,791 + GFF2680 0.29 -0.2 203,794 + GFF2680 0.29 -0.5 203,812 + GFF2680 0.31 +0.1 203,812 + GFF2680 0.31 +0.1 203,812 + GFF2680 0.31 +1.2 203,868 - GFF2680 0.36 +1.7 204,025 + GFF2680 0.51 -0.1 204,117 - GFF2680 0.59 -0.0 204,120 + GFF2680 0.60 -0.0 204,289 - GFF2680 0.76 +0.7 204,444 - +2.3 204,444 - +0.8 204,492 - -2.1 204,557 - +0.3 204,566 + +0.1 204,610 + -0.1 204,832 + GFF2681 0.21 +0.3 204,833 - GFF2681 0.21 +0.1 204,833 - GFF2681 0.21 -0.1 204,871 + GFF2681 0.23 +0.1 204,872 - GFF2681 0.23 +0.5 204,895 + GFF2681 0.25 +0.2 204,896 - GFF2681 0.25 +0.8 205,039 + GFF2681 0.35 -0.1 205,155 + GFF2681 0.44 -0.2 205,158 + GFF2681 0.44 -0.7 205,334 - GFF2681 0.56 +0.6 205,464 - GFF2681 0.66 +0.3 205,493 + GFF2681 0.68 +0.1 205,561 - GFF2681 0.73 -0.1 205,561 - GFF2681 0.73 +0.5 205,561 - GFF2681 0.73 +0.4 205,689 - GFF2681 0.82 +0.6 205,702 - GFF2681 0.83 -0.4 205,996 - +0.4 206,017 + +0.6 206,150 + GFF2682 0.19 +0.6 206,209 + GFF2682 0.37 -0.2 206,283 - GFF2682 0.61 +0.3 206,342 - GFF2682 0.80 +0.2 206,406 - +0.1 206,424 - +0.0 206,442 + +1.2 206,443 - -1.6 206,445 - +0.6 206,516 + GFF2683 0.16 +0.7 206,581 - GFF2683 0.46 +0.5 206,646 + GFF2683 0.75 +0.3 206,714 + +0.3 206,714 + -2.3 206,717 - -1.0 206,723 + -0.6 206,723 + +0.3 206,884 - GFF2684 0.45 +0.0 206,893 + GFF2684 0.47 +0.2 206,913 - GFF2684 0.53 +1.2 206,913 - GFF2684 0.53 -0.2
Or see this region's nucleotide sequence