Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF24 and GFF25 overlap by 37 nucleotides GFF25 and GFF26 are separated by 110 nucleotides GFF26 and GFF27 are separated by 73 nucleotides GFF27 and GFF28 are separated by 52 nucleotides GFF28 and GFF29 are separated by 31 nucleotides
GFF24 - Protein RcsF, at 3,958 to 4,089
GFF24
GFF25 - COG1720: Uncharacterized conserved protein, at 4,053 to 4,793
GFF25
GFF26 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 4,904 to 6,622
GFF26
GFF27 - Copper homeostasis protein CutF precursor / Lipoprotein NlpE involeved in surface adhesion, at 6,696 to 6,818
GFF27
GFF28 - Copper homeostasis protein CutF precursor / Lipoprotein NlpE involeved in surface adhesion, at 6,871 to 7,401
GFF28
GFF29 - Hypothetical protein YaeJ with similarity to translation release factor, at 7,433 to 7,855
GFF29
Position (kb) 4
5
6
7 Strain fitness (log2 ratio) -1
0
1
2 at 3.957 kb on + strand at 3.957 kb on + strand at 3.958 kb on - strand at 4.209 kb on + strand, within GFF25 at 4.405 kb on + strand, within GFF25 at 4.490 kb on + strand, within GFF25 at 4.604 kb on + strand, within GFF25 at 4.643 kb on + strand, within GFF25 at 4.686 kb on - strand, within GFF25 at 6.663 kb on - strand at 6.804 kb on + strand, within GFF27 at 6.937 kb on - strand, within GFF28 at 6.937 kb on - strand, within GFF28 at 6.937 kb on - strand, within GFF28 at 6.937 kb on - strand, within GFF28 at 6.937 kb on - strand, within GFF28 at 6.940 kb on + strand, within GFF28 at 6.940 kb on + strand, within GFF28 at 6.941 kb on - strand, within GFF28 at 6.941 kb on - strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.948 kb on + strand, within GFF28 at 6.948 kb on + strand, within GFF28 at 6.948 kb on + strand, within GFF28 at 6.949 kb on - strand, within GFF28 at 7.005 kb on - strand, within GFF28 at 7.008 kb on + strand, within GFF28 at 7.074 kb on - strand, within GFF28 at 7.140 kb on - strand, within GFF28 at 7.219 kb on + strand, within GFF28 at 7.339 kb on - strand, within GFF28 at 7.363 kb on - strand at 7.384 kb on + strand at 7.385 kb on - strand at 7.473 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 3,957 + +0.0 3,957 + +0.7 3,958 - -0.8 4,209 + GFF25 0.21 +0.0 4,405 + GFF25 0.48 +0.3 4,490 + GFF25 0.59 +0.4 4,604 + GFF25 0.74 +2.7 4,643 + GFF25 0.80 +0.3 4,686 - GFF25 0.85 +0.1 6,663 - +0.2 6,804 + GFF27 0.88 -0.4 6,937 - GFF28 0.12 +1.1 6,937 - GFF28 0.12 +0.2 6,937 - GFF28 0.12 +0.2 6,937 - GFF28 0.12 +0.5 6,937 - GFF28 0.12 -0.1 6,940 + GFF28 0.13 +0.6 6,940 + GFF28 0.13 +0.9 6,941 - GFF28 0.13 +0.4 6,941 - GFF28 0.13 +0.6 6,944 + GFF28 0.14 +0.7 6,944 + GFF28 0.14 +0.3 6,944 + GFF28 0.14 -0.6 6,944 + GFF28 0.14 -0.0 6,944 + GFF28 0.14 -0.4 6,945 - GFF28 0.14 -0.2 6,945 - GFF28 0.14 -0.3 6,945 - GFF28 0.14 -1.0 6,945 - GFF28 0.14 +0.2 6,945 - GFF28 0.14 +0.4 6,945 - GFF28 0.14 +0.5 6,948 + GFF28 0.15 -0.2 6,948 + GFF28 0.15 -0.4 6,948 + GFF28 0.15 +0.3 6,949 - GFF28 0.15 +0.9 7,005 - GFF28 0.25 -0.3 7,008 + GFF28 0.26 -0.4 7,074 - GFF28 0.38 +0.1 7,140 - GFF28 0.51 +0.2 7,219 + GFF28 0.66 -0.6 7,339 - GFF28 0.88 -0.1 7,363 - +0.4 7,384 + -0.5 7,385 - +0.2 7,473 - -0.2
Or see this region's nucleotide sequence