Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2556 and GFF2557 are separated by 285 nucleotides GFF2557 and GFF2558 are separated by 90 nucleotides GFF2558 and GFF2559 overlap by 8 nucleotides
GFF2556 - NAD-dependent malic enzyme (EC 1.1.1.38), at 71,428 to 73,125
GFF2556
GFF2557 - Alcohol dehydrogenase (EC 1.1.1.1), at 73,411 to 74,421
GFF2557
GFF2558 - Formate dehydrogenase N gamma subunit (EC 1.2.1.2), at 74,512 to 75,168
GFF2558
GFF2559 - Formate dehydrogenase N beta subunit (EC 1.2.1.2), at 75,161 to 76,045
GFF2559
Position (kb)
73
74
75 Strain fitness (log2 ratio)
-1
0
1 at 72.642 kb on + strand, within GFF2556 at 72.810 kb on + strand, within GFF2556 at 72.821 kb on - strand, within GFF2556 at 73.005 kb on - strand at 73.126 kb on + strand at 73.126 kb on + strand at 73.315 kb on + strand at 73.347 kb on - strand at 73.372 kb on - strand at 73.391 kb on - strand at 73.393 kb on + strand at 73.394 kb on - strand at 73.496 kb on + strand at 73.532 kb on + strand, within GFF2557 at 73.666 kb on + strand, within GFF2557 at 73.666 kb on + strand, within GFF2557 at 73.774 kb on - strand, within GFF2557 at 73.887 kb on + strand, within GFF2557 at 73.887 kb on + strand, within GFF2557 at 73.888 kb on - strand, within GFF2557 at 73.912 kb on + strand, within GFF2557 at 74.043 kb on + strand, within GFF2557 at 74.148 kb on - strand, within GFF2557 at 74.398 kb on - strand at 74.445 kb on + strand at 74.658 kb on - strand, within GFF2558 at 74.658 kb on - strand, within GFF2558 at 74.658 kb on - strand, within GFF2558 at 74.661 kb on + strand, within GFF2558 at 74.662 kb on - strand, within GFF2558 at 74.662 kb on - strand, within GFF2558 at 74.662 kb on - strand, within GFF2558 at 74.718 kb on + strand, within GFF2558 at 74.719 kb on - strand, within GFF2558 at 74.808 kb on + strand, within GFF2558 at 74.809 kb on - strand, within GFF2558 at 74.844 kb on - strand, within GFF2558 at 74.844 kb on - strand, within GFF2558 at 74.865 kb on + strand, within GFF2558 at 74.866 kb on - strand, within GFF2558 at 74.885 kb on - strand, within GFF2558 at 75.050 kb on - strand, within GFF2558 at 75.220 kb on - strand at 75.220 kb on - strand at 75.369 kb on + strand, within GFF2559 at 75.409 kb on + strand, within GFF2559
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 72,642 + GFF2556 0.71 +0.6 72,810 + GFF2556 0.81 +0.0 72,821 - GFF2556 0.82 +0.1 73,005 - +0.4 73,126 + -0.3 73,126 + +0.5 73,315 + -0.1 73,347 - +0.4 73,372 - -0.2 73,391 - -1.1 73,393 + +0.6 73,394 - +0.5 73,496 + -0.1 73,532 + GFF2557 0.12 +0.3 73,666 + GFF2557 0.25 +0.5 73,666 + GFF2557 0.25 -0.0 73,774 - GFF2557 0.36 -1.3 73,887 + GFF2557 0.47 -0.7 73,887 + GFF2557 0.47 -0.7 73,888 - GFF2557 0.47 -0.2 73,912 + GFF2557 0.50 -0.7 74,043 + GFF2557 0.63 -0.6 74,148 - GFF2557 0.73 -0.2 74,398 - +0.6 74,445 + -0.8 74,658 - GFF2558 0.22 +0.3 74,658 - GFF2558 0.22 +0.2 74,658 - GFF2558 0.22 -0.9 74,661 + GFF2558 0.23 -1.6 74,662 - GFF2558 0.23 +0.7 74,662 - GFF2558 0.23 +0.3 74,662 - GFF2558 0.23 +0.5 74,718 + GFF2558 0.31 +0.1 74,719 - GFF2558 0.32 -0.5 74,808 + GFF2558 0.45 -0.5 74,809 - GFF2558 0.45 -0.0 74,844 - GFF2558 0.51 +0.2 74,844 - GFF2558 0.51 -0.1 74,865 + GFF2558 0.54 -0.2 74,866 - GFF2558 0.54 -0.4 74,885 - GFF2558 0.57 -0.7 75,050 - GFF2558 0.82 +0.8 75,220 - +0.4 75,220 - +1.1 75,369 + GFF2559 0.24 -0.2 75,409 + GFF2559 0.28 +1.0
Or see this region's nucleotide sequence