Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2549

Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2548 and GFF2549 are separated by 56 nucleotidesGFF2549 and GFF2550 overlap by 4 nucleotides GFF2548 - Glycogen debranching enzyme (EC 3.2.1.-), at 61,677 to 63,752 GFF2548 GFF2549 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 63,809 to 66,337 GFF2549 GFF2550 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 66,334 to 68,118 GFF2550 Position (kb) 63 64 65 66 67Strain fitness (log2 ratio) -2 -1 0 1at 62.822 kb on - strand, within GFF2548at 62.854 kb on + strand, within GFF2548at 62.923 kb on + strand, within GFF2548at 63.011 kb on - strand, within GFF2548at 63.047 kb on - strand, within GFF2548at 63.128 kb on + strand, within GFF2548at 63.251 kb on + strand, within GFF2548at 63.329 kb on + strand, within GFF2548at 63.330 kb on - strand, within GFF2548at 63.445 kb on + strand, within GFF2548at 63.445 kb on + strand, within GFF2548at 63.445 kb on + strand, within GFF2548at 63.578 kb on - strandat 63.876 kb on - strandat 63.890 kb on - strandat 63.934 kb on + strandat 64.096 kb on + strand, within GFF2549at 64.096 kb on + strand, within GFF2549at 64.097 kb on - strand, within GFF2549at 64.097 kb on - strand, within GFF2549at 64.097 kb on - strand, within GFF2549at 64.099 kb on - strand, within GFF2549at 64.315 kb on + strand, within GFF2549at 64.316 kb on - strand, within GFF2549at 64.316 kb on - strand, within GFF2549at 64.750 kb on + strand, within GFF2549at 64.753 kb on - strand, within GFF2549at 64.904 kb on + strand, within GFF2549at 65.117 kb on + strand, within GFF2549at 65.161 kb on - strand, within GFF2549at 65.186 kb on + strand, within GFF2549at 65.187 kb on - strand, within GFF2549at 65.360 kb on + strand, within GFF2549at 65.391 kb on - strand, within GFF2549at 65.553 kb on + strand, within GFF2549at 65.554 kb on - strand, within GFF2549at 65.554 kb on - strand, within GFF2549at 65.645 kb on - strand, within GFF2549at 65.681 kb on + strand, within GFF2549at 65.783 kb on - strand, within GFF2549at 65.854 kb on - strand, within GFF2549at 65.858 kb on - strand, within GFF2549at 65.858 kb on - strand, within GFF2549at 65.865 kb on + strand, within GFF2549at 66.151 kb on + strandat 66.231 kb on + strandat 66.232 kb on - strandat 66.262 kb on - strandat 66.262 kb on - strandat 66.319 kb on - strandat 66.369 kb on + strandat 66.449 kb on - strandat 66.466 kb on - strandat 67.038 kb on - strand, within GFF2550at 67.276 kb on - strand, within GFF2550at 67.311 kb on + strand, within GFF2550

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI
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62,822 - GFF2548 0.55 +0.2
62,854 + GFF2548 0.57 +0.0
62,923 + GFF2548 0.60 -0.7
63,011 - GFF2548 0.64 -2.0
63,047 - GFF2548 0.66 +0.2
63,128 + GFF2548 0.70 -1.1
63,251 + GFF2548 0.76 +0.2
63,329 + GFF2548 0.80 +0.3
63,330 - GFF2548 0.80 +0.2
63,445 + GFF2548 0.85 -0.8
63,445 + GFF2548 0.85 -0.4
63,445 + GFF2548 0.85 +0.1
63,578 - -1.8
63,876 - +0.5
63,890 - +0.6
63,934 + -0.2
64,096 + GFF2549 0.11 -1.0
64,096 + GFF2549 0.11 +0.7
64,097 - GFF2549 0.11 -0.4
64,097 - GFF2549 0.11 +0.2
64,097 - GFF2549 0.11 -0.2
64,099 - GFF2549 0.11 +1.2
64,315 + GFF2549 0.20 -0.1
64,316 - GFF2549 0.20 +0.5
64,316 - GFF2549 0.20 -0.2
64,750 + GFF2549 0.37 +0.6
64,753 - GFF2549 0.37 -0.1
64,904 + GFF2549 0.43 -0.7
65,117 + GFF2549 0.52 +0.7
65,161 - GFF2549 0.53 -1.4
65,186 + GFF2549 0.54 +0.8
65,187 - GFF2549 0.54 -0.4
65,360 + GFF2549 0.61 +0.4
65,391 - GFF2549 0.63 +0.3
65,553 + GFF2549 0.69 -0.0
65,554 - GFF2549 0.69 +0.1
65,554 - GFF2549 0.69 +0.1
65,645 - GFF2549 0.73 +0.6
65,681 + GFF2549 0.74 -0.3
65,783 - GFF2549 0.78 +0.3
65,854 - GFF2549 0.81 +0.6
65,858 - GFF2549 0.81 +0.3
65,858 - GFF2549 0.81 +0.5
65,865 + GFF2549 0.81 -0.1
66,151 + -0.7
66,231 + +0.2
66,232 - +0.8
66,262 - -0.2
66,262 - -1.0
66,319 - -0.1
66,369 + -0.7
66,449 - -1.0
66,466 - -0.4
67,038 - GFF2550 0.39 -0.3
67,276 - GFF2550 0.53 -0.3
67,311 + GFF2550 0.55 +0.1

Or see this region's nucleotide sequence