Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2525

Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2524 and GFF2525 are separated by 2 nucleotidesGFF2525 and GFF2526 are separated by 238 nucleotidesGFF2526 and GFF2527 are separated by 148 nucleotides GFF2524 - Uptake hydrogenase large subunit (EC 1.12.99.6), at 37,613 to 39,415 GFF2524 GFF2525 - Uptake hydrogenase small subunit precursor (EC 1.12.99.6), at 39,418 to 40,410 GFF2525 GFF2526 - hypothetical protein, at 40,649 to 40,801 GFF2526 GFF2527 - Choloylglycine hydrolase (EC 3.5.1.24), at 40,950 to 42,041 GFF2527 Position (kb) 39 40 41Strain fitness (log2 ratio) -1 0 1at 38.440 kb on + strand, within GFF2524at 38.860 kb on - strand, within GFF2524at 38.860 kb on - strand, within GFF2524at 39.074 kb on + strand, within GFF2524at 39.079 kb on + strand, within GFF2524at 39.091 kb on + strand, within GFF2524at 39.296 kb on - strandat 39.296 kb on - strandat 39.608 kb on + strand, within GFF2525at 39.705 kb on + strand, within GFF2525at 39.738 kb on - strand, within GFF2525at 39.903 kb on - strand, within GFF2525at 39.903 kb on - strand, within GFF2525at 39.999 kb on + strand, within GFF2525at 40.188 kb on - strand, within GFF2525at 40.357 kb on + strandat 40.378 kb on - strandat 40.392 kb on - strandat 40.397 kb on - strandat 40.397 kb on - strandat 40.542 kb on - strandat 40.543 kb on + strandat 40.722 kb on - strand, within GFF2526at 41.322 kb on + strand, within GFF2527

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI
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38,440 + GFF2524 0.46 +0.1
38,860 - GFF2524 0.69 -0.2
38,860 - GFF2524 0.69 +0.0
39,074 + GFF2524 0.81 +0.5
39,079 + GFF2524 0.81 +0.4
39,091 + GFF2524 0.82 +0.3
39,296 - -0.9
39,296 - +0.5
39,608 + GFF2525 0.19 +0.2
39,705 + GFF2525 0.29 +0.1
39,738 - GFF2525 0.32 +0.3
39,903 - GFF2525 0.49 +0.2
39,903 - GFF2525 0.49 +0.0
39,999 + GFF2525 0.59 +0.7
40,188 - GFF2525 0.78 +0.2
40,357 + +0.2
40,378 - -1.3
40,392 - +0.8
40,397 - +0.5
40,397 - -1.5
40,542 - -0.7
40,543 + -0.2
40,722 - GFF2526 0.48 +0.1
41,322 + GFF2527 0.34 +0.4

Or see this region's nucleotide sequence