Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2403 and GFF2404 are separated by 137 nucleotides GFF2404 and GFF2405 overlap by 46 nucleotides GFF2405 and GFF2406 overlap by 20 nucleotides
GFF2403 - RatA homolog, at 10,359 to 15,938
GFF2403
GFF2404 - SinI homolog, at 16,076 to 17,035
GFF2404
GFF2405 - hypothetical protein, at 16,990 to 17,112
GFF2405
GFF2406 - 'adherence and invasion outermembrane protein (Inv,enhances Peyer's patches colonization)' transl_table=11, at 17,093 to 19,285
GFF2406
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-1
0
1 at 15.390 kb on + strand at 15.432 kb on - strand at 15.432 kb on - strand at 15.761 kb on + strand at 16.077 kb on + strand at 16.089 kb on + strand at 16.243 kb on + strand, within GFF2404 at 16.282 kb on - strand, within GFF2404 at 16.317 kb on + strand, within GFF2404 at 16.317 kb on + strand, within GFF2404 at 16.317 kb on + strand, within GFF2404 at 16.317 kb on + strand, within GFF2404 at 16.321 kb on + strand, within GFF2404 at 16.353 kb on - strand, within GFF2404 at 16.353 kb on - strand, within GFF2404 at 16.359 kb on + strand, within GFF2404 at 16.360 kb on - strand, within GFF2404 at 16.555 kb on + strand, within GFF2404 at 16.585 kb on - strand, within GFF2404 at 16.757 kb on - strand, within GFF2404 at 16.816 kb on + strand, within GFF2404 at 16.817 kb on - strand, within GFF2404 at 16.826 kb on + strand, within GFF2404 at 16.826 kb on + strand, within GFF2404 at 16.994 kb on + strand at 16.994 kb on + strand at 17.094 kb on + strand at 17.104 kb on + strand at 17.106 kb on + strand at 17.106 kb on + strand at 17.106 kb on + strand at 17.106 kb on + strand at 17.106 kb on + strand at 17.106 kb on + strand at 17.107 kb on - strand at 17.107 kb on - strand at 17.107 kb on - strand at 17.110 kb on + strand at 17.118 kb on - strand at 17.383 kb on + strand, within GFF2406 at 17.383 kb on + strand, within GFF2406 at 17.399 kb on - strand, within GFF2406 at 17.484 kb on + strand, within GFF2406 at 17.485 kb on - strand, within GFF2406 at 17.485 kb on - strand, within GFF2406 at 17.489 kb on - strand, within GFF2406 at 18.019 kb on + strand, within GFF2406 at 18.019 kb on + strand, within GFF2406 at 18.027 kb on - strand, within GFF2406
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 15,390 + +0.7 15,432 - +0.4 15,432 - -0.0 15,761 + -0.1 16,077 + -0.6 16,089 + +0.4 16,243 + GFF2404 0.17 -0.3 16,282 - GFF2404 0.21 -1.2 16,317 + GFF2404 0.25 -1.2 16,317 + GFF2404 0.25 -0.4 16,317 + GFF2404 0.25 -0.9 16,317 + GFF2404 0.25 +0.2 16,321 + GFF2404 0.26 +0.1 16,353 - GFF2404 0.29 -0.1 16,353 - GFF2404 0.29 +0.1 16,359 + GFF2404 0.29 +0.3 16,360 - GFF2404 0.30 -1.4 16,555 + GFF2404 0.50 -0.4 16,585 - GFF2404 0.53 -0.4 16,757 - GFF2404 0.71 +0.9 16,816 + GFF2404 0.77 -1.3 16,817 - GFF2404 0.77 +1.0 16,826 + GFF2404 0.78 -0.4 16,826 + GFF2404 0.78 +0.1 16,994 + -0.6 16,994 + -1.1 17,094 + +0.7 17,104 + -0.7 17,106 + +0.8 17,106 + +0.9 17,106 + -0.1 17,106 + +0.3 17,106 + -1.1 17,106 + -0.3 17,107 - -0.5 17,107 - -0.2 17,107 - +0.2 17,110 + -0.4 17,118 - +0.1 17,383 + GFF2406 0.13 -0.7 17,383 + GFF2406 0.13 +0.1 17,399 - GFF2406 0.14 +0.4 17,484 + GFF2406 0.18 -0.2 17,485 - GFF2406 0.18 -0.4 17,485 - GFF2406 0.18 +0.5 17,489 - GFF2406 0.18 +0.8 18,019 + GFF2406 0.42 +0.3 18,019 + GFF2406 0.42 +0.8 18,027 - GFF2406 0.43 -0.0
Or see this region's nucleotide sequence