Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2253 and GFF2254 are separated by 320 nucleotides GFF2254 and GFF2255 are separated by 267 nucleotides
GFF2253 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 43,682 to 46,732
GFF2253
GFF2254 - Formate dehydrogenase chain D (EC 1.2.1.2), at 47,053 to 47,763
GFF2254
GFF2255 - Putative lipoprotein, at 48,031 to 49,062
GFF2255
Position (kb)
47
48 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 46.223 kb on - strand, within GFF2253 at 46.331 kb on - strand, within GFF2253 at 46.376 kb on - strand, within GFF2253 at 46.429 kb on + strand at 46.511 kb on - strand at 46.620 kb on + strand at 46.877 kb on - strand at 46.885 kb on - strand at 46.911 kb on - strand at 46.911 kb on - strand at 46.911 kb on - strand at 46.911 kb on - strand at 46.912 kb on + strand at 46.913 kb on - strand at 46.935 kb on + strand at 46.935 kb on + strand at 46.936 kb on - strand at 46.937 kb on + strand at 46.956 kb on - strand at 47.065 kb on + strand at 47.066 kb on - strand at 47.066 kb on - strand at 47.066 kb on - strand at 47.294 kb on + strand, within GFF2254 at 47.294 kb on + strand, within GFF2254 at 47.295 kb on - strand, within GFF2254 at 47.325 kb on + strand, within GFF2254 at 47.433 kb on + strand, within GFF2254 at 47.514 kb on + strand, within GFF2254 at 47.591 kb on - strand, within GFF2254 at 47.709 kb on + strand at 47.731 kb on + strand at 47.809 kb on + strand at 47.837 kb on + strand at 47.837 kb on + strand at 47.837 kb on + strand at 47.837 kb on + strand at 47.837 kb on + strand at 48.003 kb on + strand at 48.003 kb on + strand at 48.003 kb on + strand at 48.003 kb on + strand at 48.004 kb on - strand at 48.033 kb on - strand at 48.060 kb on + strand at 48.060 kb on + strand at 48.061 kb on - strand at 48.061 kb on - strand at 48.062 kb on + strand at 48.063 kb on - strand at 48.083 kb on + strand at 48.105 kb on - strand at 48.105 kb on - strand at 48.115 kb on + strand at 48.116 kb on - strand at 48.116 kb on - strand at 48.116 kb on - strand at 48.116 kb on - strand at 48.164 kb on + strand, within GFF2255 at 48.164 kb on + strand, within GFF2255 at 48.263 kb on - strand, within GFF2255 at 48.263 kb on - strand, within GFF2255 at 48.298 kb on - strand, within GFF2255 at 48.358 kb on + strand, within GFF2255 at 48.405 kb on - strand, within GFF2255 at 48.407 kb on + strand, within GFF2255 at 48.495 kb on - strand, within GFF2255 at 48.586 kb on - strand, within GFF2255 at 48.586 kb on - strand, within GFF2255 at 48.613 kb on - strand, within GFF2255 at 48.648 kb on + strand, within GFF2255 at 48.691 kb on + strand, within GFF2255
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 46,223 - GFF2253 0.83 +0.7 46,331 - GFF2253 0.87 -0.1 46,376 - GFF2253 0.88 +0.8 46,429 + +0.1 46,511 - +0.6 46,620 + +1.2 46,877 - -0.3 46,885 - +0.6 46,911 - +0.3 46,911 - -0.3 46,911 - +0.4 46,911 - +0.4 46,912 + +0.2 46,913 - +0.0 46,935 + +0.6 46,935 + +0.2 46,936 - +0.4 46,937 + +0.1 46,956 - -0.1 47,065 + +0.5 47,066 - +0.4 47,066 - +0.5 47,066 - +0.2 47,294 + GFF2254 0.34 +0.3 47,294 + GFF2254 0.34 +1.1 47,295 - GFF2254 0.34 +0.2 47,325 + GFF2254 0.38 +0.5 47,433 + GFF2254 0.53 +0.4 47,514 + GFF2254 0.65 +2.0 47,591 - GFF2254 0.76 +0.2 47,709 + -0.6 47,731 + -0.0 47,809 + +0.1 47,837 + -0.3 47,837 + +0.3 47,837 + -0.0 47,837 + +0.3 47,837 + +0.8 48,003 + -0.2 48,003 + +0.1 48,003 + -0.2 48,003 + -1.0 48,004 - -1.0 48,033 - -0.1 48,060 + -0.6 48,060 + +0.4 48,061 - -1.1 48,061 - +0.1 48,062 + +0.5 48,063 - -0.0 48,083 + -0.0 48,105 - +1.8 48,105 - -0.7 48,115 + +0.2 48,116 - -0.6 48,116 - -0.0 48,116 - -0.0 48,116 - -1.0 48,164 + GFF2255 0.13 +0.2 48,164 + GFF2255 0.13 -0.4 48,263 - GFF2255 0.22 -0.3 48,263 - GFF2255 0.22 -1.9 48,298 - GFF2255 0.26 +0.3 48,358 + GFF2255 0.32 -0.1 48,405 - GFF2255 0.36 +0.3 48,407 + GFF2255 0.36 -0.8 48,495 - GFF2255 0.45 -0.4 48,586 - GFF2255 0.54 +0.2 48,586 - GFF2255 0.54 -0.2 48,613 - GFF2255 0.56 +0.2 48,648 + GFF2255 0.60 +1.1 48,691 + GFF2255 0.64 -0.9
Or see this region's nucleotide sequence