Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2214

Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2212 and GFF2213 are separated by 16 nucleotidesGFF2213 and GFF2214 are separated by 12 nucleotidesGFF2214 and GFF2215 are separated by 242 nucleotidesGFF2215 and GFF2216 are separated by 58 nucleotides GFF2212 - YihE protein, a ser/thr kinase implicated in LPS synthesis and Cpx signalling, at 2,120 to 3,106 GFF2212 GFF2213 - Periplasmic thiol:disulfide interchange protein DsbA, at 3,123 to 3,746 GFF2213 GFF2214 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 3,759 to 4,622 GFF2214 GFF2215 - hypothetical protein, at 4,865 to 4,996 GFF2215 GFF2216 - DNA polymerase I (EC 2.7.7.7), at 5,055 to 7,841 GFF2216 Position (kb) 3 4 5Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2.945 kb on + strand, within GFF2212at 2.946 kb on - strand, within GFF2212at 3.152 kb on + strandat 3.167 kb on + strandat 3.206 kb on + strand, within GFF2213at 3.206 kb on + strand, within GFF2213at 3.264 kb on + strand, within GFF2213at 3.428 kb on - strand, within GFF2213at 3.435 kb on - strand, within GFF2213at 3.482 kb on - strand, within GFF2213at 3.482 kb on - strand, within GFF2213at 3.482 kb on - strand, within GFF2213at 3.541 kb on - strand, within GFF2213at 3.660 kb on - strand, within GFF2213at 3.798 kb on + strandat 3.828 kb on - strandat 3.830 kb on + strandat 3.951 kb on - strand, within GFF2214at 3.956 kb on - strand, within GFF2214at 3.993 kb on - strand, within GFF2214at 3.993 kb on - strand, within GFF2214at 4.032 kb on - strand, within GFF2214at 4.229 kb on - strand, within GFF2214at 4.260 kb on - strand, within GFF2214at 4.264 kb on - strand, within GFF2214at 4.326 kb on - strand, within GFF2214at 4.391 kb on + strand, within GFF2214at 4.392 kb on - strand, within GFF2214at 4.392 kb on - strand, within GFF2214at 4.401 kb on + strand, within GFF2214at 4.440 kb on + strand, within GFF2214at 4.441 kb on - strand, within GFF2214at 4.501 kb on + strand, within GFF2214at 4.501 kb on + strand, within GFF2214at 4.502 kb on - strand, within GFF2214at 4.502 kb on - strand, within GFF2214at 4.502 kb on - strand, within GFF2214at 4.542 kb on - strandat 4.670 kb on + strandat 4.744 kb on + strandat 4.793 kb on + strandat 5.009 kb on + strandat 5.010 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI
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2,945 + GFF2212 0.84 +0.1
2,946 - GFF2212 0.84 -0.2
3,152 + -5.4
3,167 + -2.7
3,206 + GFF2213 0.13 -4.8
3,206 + GFF2213 0.13 -3.9
3,264 + GFF2213 0.23 -4.7
3,428 - GFF2213 0.49 -4.8
3,435 - GFF2213 0.50 -4.0
3,482 - GFF2213 0.58 -2.6
3,482 - GFF2213 0.58 -3.0
3,482 - GFF2213 0.58 -2.8
3,541 - GFF2213 0.67 -3.3
3,660 - GFF2213 0.86 -5.0
3,798 + -0.1
3,828 - -0.2
3,830 + +0.2
3,951 - GFF2214 0.22 +0.2
3,956 - GFF2214 0.23 -0.2
3,993 - GFF2214 0.27 -0.1
3,993 - GFF2214 0.27 -0.8
4,032 - GFF2214 0.32 -1.0
4,229 - GFF2214 0.54 -0.5
4,260 - GFF2214 0.58 -0.5
4,264 - GFF2214 0.58 -0.3
4,326 - GFF2214 0.66 +0.3
4,391 + GFF2214 0.73 -0.9
4,392 - GFF2214 0.73 -1.1
4,392 - GFF2214 0.73 +0.1
4,401 + GFF2214 0.74 -1.3
4,440 + GFF2214 0.79 -0.4
4,441 - GFF2214 0.79 -0.2
4,501 + GFF2214 0.86 +0.1
4,501 + GFF2214 0.86 +0.7
4,502 - GFF2214 0.86 -0.8
4,502 - GFF2214 0.86 +0.3
4,502 - GFF2214 0.86 +0.1
4,542 - -0.1
4,670 + -0.7
4,744 + +0.2
4,793 + +0.4
5,009 + +0.1
5,010 - -0.3

Or see this region's nucleotide sequence