Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2020 and GFF2021 are separated by 155 nucleotides GFF2021 and GFF2022 overlap by 39 nucleotides GFF2022 and GFF2023 are separated by 380 nucleotides GFF2023 and GFF2024 are separated by 42 nucleotides GFF2024 and GFF2025 are separated by 247 nucleotides GFF2025 and GFF2026 overlap by 4 nucleotides
GFF2020 - hypothetical protein, at 30,962 to 31,156
GFF2020
GFF2021 - FIG01049650: hypothetical protein, at 31,312 to 31,467
GFF2021
GFF2022 - hypothetical protein, at 31,429 to 31,575
GFF2022
GFF2023 - hypothetical protein, at 31,956 to 32,090
GFF2023
GFF2024 - Nonspecific acid phosphatase precursor (EC 3.1.3.2), at 32,133 to 32,885
GFF2024
GFF2025 - FIG001353: Acetyltransferase, at 33,133 to 33,624
GFF2025
GFF2026 - FIG032766: hypothetical protein, at 33,621 to 33,914
GFF2026
Position (kb) 32
33 Strain fitness (log2 ratio) -1
0
1 at 31.266 kb on + strand at 31.267 kb on - strand at 31.344 kb on + strand, within GFF2021 at 31.472 kb on + strand, within GFF2022 at 31.472 kb on + strand, within GFF2022 at 31.472 kb on + strand, within GFF2022 at 31.482 kb on + strand, within GFF2022 at 31.531 kb on - strand, within GFF2022 at 31.542 kb on + strand, within GFF2022 at 31.596 kb on + strand at 31.597 kb on - strand at 32.452 kb on + strand, within GFF2024 at 32.570 kb on + strand, within GFF2024 at 32.571 kb on - strand, within GFF2024 at 32.613 kb on + strand, within GFF2024 at 32.618 kb on + strand, within GFF2024 at 32.764 kb on + strand, within GFF2024 at 32.848 kb on + strand at 33.084 kb on - strand at 33.114 kb on - strand at 33.197 kb on - strand, within GFF2025 at 33.270 kb on + strand, within GFF2025 at 33.396 kb on + strand, within GFF2025 at 33.456 kb on - strand, within GFF2025 at 33.467 kb on - strand, within GFF2025 at 33.550 kb on - strand, within GFF2025 at 33.553 kb on + strand, within GFF2025 at 33.554 kb on - strand, within GFF2025 at 33.582 kb on + strand at 33.583 kb on - strand at 33.583 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 31,266 + -0.0 31,267 - +0.7 31,344 + GFF2021 0.21 +0.2 31,472 + GFF2022 0.29 -1.2 31,472 + GFF2022 0.29 -1.0 31,472 + GFF2022 0.29 +0.2 31,482 + GFF2022 0.36 -1.4 31,531 - GFF2022 0.69 -0.7 31,542 + GFF2022 0.77 -0.5 31,596 + -0.7 31,597 - -0.7 32,452 + GFF2024 0.42 -0.7 32,570 + GFF2024 0.58 +0.2 32,571 - GFF2024 0.58 -0.0 32,613 + GFF2024 0.64 -0.9 32,618 + GFF2024 0.64 +0.1 32,764 + GFF2024 0.84 -0.1 32,848 + +0.5 33,084 - +0.1 33,114 - -0.9 33,197 - GFF2025 0.13 +0.1 33,270 + GFF2025 0.28 -0.2 33,396 + GFF2025 0.53 -0.7 33,456 - GFF2025 0.66 +0.6 33,467 - GFF2025 0.68 +0.2 33,550 - GFF2025 0.85 +0.5 33,553 + GFF2025 0.85 -0.1 33,554 - GFF2025 0.86 -0.0 33,582 + +0.4 33,583 - -0.0 33,583 - +0.1
Or see this region's nucleotide sequence