Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1903 and GFF1904 overlap by 8 nucleotides GFF1904 and GFF1905 overlap by 4 nucleotides GFF1905 and GFF1906 are separated by 20 nucleotides GFF1906 and GFF1907 are separated by 80 nucleotides GFF1907 and GFF1908 overlap by 2 nucleotides
GFF1903 - Transketolase, C-terminal section (EC 2.2.1.1), at 99,539 to 100,492
GFF1903
GFF1904 - Transketolase, N-terminal section (EC 2.2.1.1), at 100,485 to 101,315
GFF1904
GFF1905 - FIG00732228: membrane protein, at 101,312 to 102,703
GFF1905
GFF1906 - Putative sugar phosphotransferase component II B, at 102,724 to 102,996
GFF1906
GFF1907 - Ascorbate-specific PTS system, EIIA component (EC 2.7.1.-), at 103,077 to 103,520
GFF1907
GFF1908 - FIG01046667: hypothetical protein, at 103,519 to 103,692
GFF1908
Position (kb) 101
102
103 Strain fitness (log2 ratio) -2
-1
0
1 at 100.374 kb on - strand, within GFF1903 at 100.393 kb on + strand, within GFF1903 at 100.466 kb on - strand at 100.487 kb on - strand at 100.672 kb on - strand, within GFF1904 at 100.922 kb on + strand, within GFF1904 at 100.949 kb on + strand, within GFF1904 at 101.048 kb on + strand, within GFF1904 at 101.095 kb on + strand, within GFF1904 at 101.127 kb on + strand, within GFF1904 at 101.558 kb on + strand, within GFF1905 at 101.561 kb on + strand, within GFF1905 at 101.561 kb on + strand, within GFF1905 at 101.569 kb on + strand, within GFF1905 at 101.769 kb on + strand, within GFF1905 at 101.769 kb on + strand, within GFF1905 at 101.886 kb on - strand, within GFF1905 at 101.931 kb on + strand, within GFF1905 at 102.001 kb on + strand, within GFF1905 at 102.029 kb on - strand, within GFF1905 at 102.176 kb on - strand, within GFF1905 at 102.187 kb on + strand, within GFF1905 at 102.187 kb on + strand, within GFF1905 at 102.190 kb on - strand, within GFF1905 at 102.343 kb on - strand, within GFF1905 at 103.355 kb on - strand, within GFF1907 at 103.482 kb on + strand at 103.482 kb on + strand at 103.524 kb on - strand at 103.580 kb on + strand, within GFF1908 at 103.580 kb on + strand, within GFF1908 at 103.700 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 100,374 - GFF1903 0.88 -0.0 100,393 + GFF1903 0.90 -1.1 100,466 - -0.0 100,487 - +0.0 100,672 - GFF1904 0.23 +0.5 100,922 + GFF1904 0.53 -0.4 100,949 + GFF1904 0.56 -0.3 101,048 + GFF1904 0.68 -0.7 101,095 + GFF1904 0.73 -0.2 101,127 + GFF1904 0.77 -0.2 101,558 + GFF1905 0.18 -0.4 101,561 + GFF1905 0.18 -1.3 101,561 + GFF1905 0.18 -1.1 101,569 + GFF1905 0.18 -0.8 101,769 + GFF1905 0.33 -2.0 101,769 + GFF1905 0.33 +0.6 101,886 - GFF1905 0.41 -0.0 101,931 + GFF1905 0.44 -0.2 102,001 + GFF1905 0.49 +0.2 102,029 - GFF1905 0.52 -0.4 102,176 - GFF1905 0.62 -0.2 102,187 + GFF1905 0.63 +0.3 102,187 + GFF1905 0.63 -2.6 102,190 - GFF1905 0.63 +0.6 102,343 - GFF1905 0.74 -0.7 103,355 - GFF1907 0.63 +0.4 103,482 + -0.2 103,482 + +0.3 103,524 - +0.1 103,580 + GFF1908 0.35 -0.8 103,580 + GFF1908 0.35 -0.8 103,700 - +0.1
Or see this region's nucleotide sequence