Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1842 and GFF1843 are separated by 115 nucleotides GFF1843 and GFF1844 are separated by 4 nucleotides
GFF1842 - LysR family transcriptional regulator STM2281, at 32,929 to 33,807
GFF1842
GFF1843 - Glycerophosphoryl diester phosphodiesterase, periplasmic (EC 3.1.4.46), at 33,923 to 34,993
GFF1843
GFF1844 - Glycerol-3-phosphate transporter, at 34,998 to 36,356
GFF1844
Position (kb) 33
34
35 Strain fitness (log2 ratio) -1
0
1 at 33.004 kb on + strand at 33.005 kb on - strand at 33.068 kb on + strand, within GFF1842 at 33.319 kb on - strand, within GFF1842 at 33.352 kb on - strand, within GFF1842 at 33.387 kb on + strand, within GFF1842 at 33.804 kb on - strand at 33.805 kb on + strand at 33.997 kb on + strand at 33.998 kb on - strand at 33.999 kb on + strand at 33.999 kb on + strand at 34.012 kb on - strand at 34.077 kb on - strand, within GFF1843 at 34.085 kb on - strand, within GFF1843 at 34.157 kb on - strand, within GFF1843 at 34.157 kb on - strand, within GFF1843 at 34.231 kb on - strand, within GFF1843 at 34.236 kb on + strand, within GFF1843 at 34.237 kb on - strand, within GFF1843 at 34.291 kb on - strand, within GFF1843 at 34.291 kb on - strand, within GFF1843 at 34.564 kb on + strand, within GFF1843 at 34.696 kb on - strand, within GFF1843 at 34.865 kb on - strand, within GFF1843 at 34.999 kb on + strand at 35.138 kb on + strand, within GFF1844 at 35.139 kb on - strand, within GFF1844 at 35.139 kb on - strand, within GFF1844 at 35.272 kb on + strand, within GFF1844 at 35.298 kb on - strand, within GFF1844 at 35.298 kb on - strand, within GFF1844 at 35.298 kb on - strand, within GFF1844 at 35.458 kb on + strand, within GFF1844 at 35.587 kb on + strand, within GFF1844 at 35.588 kb on - strand, within GFF1844 at 35.615 kb on - strand, within GFF1844 at 35.784 kb on - strand, within GFF1844 at 35.786 kb on - strand, within GFF1844
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 33,004 + -0.3 33,005 - +0.3 33,068 + GFF1842 0.16 -0.5 33,319 - GFF1842 0.44 +0.9 33,352 - GFF1842 0.48 -0.7 33,387 + GFF1842 0.52 -0.1 33,804 - -0.2 33,805 + +0.4 33,997 + -1.4 33,998 - +0.6 33,999 + -0.6 33,999 + -0.6 34,012 - -0.4 34,077 - GFF1843 0.14 -0.4 34,085 - GFF1843 0.15 +0.2 34,157 - GFF1843 0.22 -0.0 34,157 - GFF1843 0.22 +0.8 34,231 - GFF1843 0.29 +0.9 34,236 + GFF1843 0.29 +0.6 34,237 - GFF1843 0.29 +0.2 34,291 - GFF1843 0.34 +0.0 34,291 - GFF1843 0.34 -0.7 34,564 + GFF1843 0.60 -1.1 34,696 - GFF1843 0.72 +0.4 34,865 - GFF1843 0.88 +1.4 34,999 + +0.4 35,138 + GFF1844 0.10 +0.5 35,139 - GFF1844 0.10 +0.2 35,139 - GFF1844 0.10 -0.4 35,272 + GFF1844 0.20 +1.0 35,298 - GFF1844 0.22 -0.0 35,298 - GFF1844 0.22 +1.1 35,298 - GFF1844 0.22 +0.4 35,458 + GFF1844 0.34 +0.3 35,587 + GFF1844 0.43 +0.6 35,588 - GFF1844 0.43 +0.2 35,615 - GFF1844 0.45 +1.1 35,784 - GFF1844 0.58 -0.1 35,786 - GFF1844 0.58 -0.2
Or see this region's nucleotide sequence