Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1825 and GFF1826 are separated by 75 nucleotides GFF1826 and GFF1827 are separated by 2 nucleotides
GFF1825 - Type I secretion system ATPase, LssB family LapB, at 7,155 to 8,798
GFF1825
GFF1826 - Alkylated DNA repair protein AlkB, at 8,874 to 9,524
GFF1826
GFF1827 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 9,527 to 10,585
GFF1827
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-1
0
1 at 7.997 kb on - strand, within GFF1825 at 8.138 kb on - strand, within GFF1825 at 8.243 kb on - strand, within GFF1825 at 8.243 kb on - strand, within GFF1825 at 8.249 kb on + strand, within GFF1825 at 8.250 kb on - strand, within GFF1825 at 8.444 kb on + strand, within GFF1825 at 8.445 kb on - strand, within GFF1825 at 8.445 kb on - strand, within GFF1825 at 8.447 kb on + strand, within GFF1825 at 8.448 kb on - strand, within GFF1825 at 8.802 kb on + strand at 8.802 kb on + strand at 8.860 kb on - strand at 9.018 kb on + strand, within GFF1826 at 9.019 kb on - strand, within GFF1826 at 9.228 kb on + strand, within GFF1826 at 9.229 kb on - strand, within GFF1826 at 9.229 kb on - strand, within GFF1826 at 9.266 kb on + strand, within GFF1826 at 9.267 kb on - strand, within GFF1826 at 9.292 kb on + strand, within GFF1826 at 9.292 kb on + strand, within GFF1826 at 9.427 kb on - strand, within GFF1826 at 9.529 kb on - strand at 9.557 kb on + strand at 9.912 kb on - strand, within GFF1827 at 10.095 kb on + strand, within GFF1827 at 10.095 kb on + strand, within GFF1827 at 10.096 kb on - strand, within GFF1827 at 10.099 kb on + strand, within GFF1827 at 10.100 kb on - strand, within GFF1827 at 10.100 kb on - strand, within GFF1827 at 10.100 kb on - strand, within GFF1827 at 10.100 kb on - strand, within GFF1827 at 10.333 kb on + strand, within GFF1827
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 7,997 - GFF1825 0.51 +0.1 8,138 - GFF1825 0.60 +0.2 8,243 - GFF1825 0.66 -0.6 8,243 - GFF1825 0.66 -0.1 8,249 + GFF1825 0.67 +0.3 8,250 - GFF1825 0.67 +0.2 8,444 + GFF1825 0.78 +0.1 8,445 - GFF1825 0.78 -0.0 8,445 - GFF1825 0.78 +0.5 8,447 + GFF1825 0.79 +0.0 8,448 - GFF1825 0.79 -1.5 8,802 + -0.5 8,802 + -0.4 8,860 - -0.5 9,018 + GFF1826 0.22 +1.0 9,019 - GFF1826 0.22 +0.6 9,228 + GFF1826 0.54 +0.0 9,229 - GFF1826 0.55 +0.2 9,229 - GFF1826 0.55 +0.3 9,266 + GFF1826 0.60 +0.8 9,267 - GFF1826 0.60 -0.7 9,292 + GFF1826 0.64 -0.2 9,292 + GFF1826 0.64 +0.3 9,427 - GFF1826 0.85 +0.3 9,529 - -0.3 9,557 + +0.1 9,912 - GFF1827 0.36 -0.1 10,095 + GFF1827 0.54 +0.3 10,095 + GFF1827 0.54 -0.4 10,096 - GFF1827 0.54 -0.1 10,099 + GFF1827 0.54 -0.3 10,100 - GFF1827 0.54 +0.5 10,100 - GFF1827 0.54 +0.4 10,100 - GFF1827 0.54 -0.7 10,100 - GFF1827 0.54 -0.3 10,333 + GFF1827 0.76 -0.3
Or see this region's nucleotide sequence