Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1797 and GFF1798 are separated by 298 nucleotides GFF1798 and GFF1799 are separated by 61 nucleotides GFF1799 and GFF1800 are separated by 135 nucleotides
GFF1797 - Protein ydgH precursor, at 18,615 to 19,559
GFF1797
GFF1798 - Arginine/ornithine antiporter ArcD, at 19,858 to 21,240
GFF1798
GFF1799 - Putative inner membrane protein, at 21,302 to 21,628
GFF1799
GFF1800 - Transcriptional regulatory protein RstA, at 21,764 to 22,495
GFF1800
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -2
-1
0
1
2 at 18.879 kb on + strand, within GFF1797 at 18.881 kb on + strand, within GFF1797 at 18.882 kb on - strand, within GFF1797 at 19.003 kb on - strand, within GFF1797 at 19.062 kb on + strand, within GFF1797 at 19.072 kb on - strand, within GFF1797 at 19.127 kb on + strand, within GFF1797 at 19.128 kb on - strand, within GFF1797 at 19.128 kb on - strand, within GFF1797 at 19.409 kb on + strand, within GFF1797 at 19.409 kb on + strand, within GFF1797 at 19.489 kb on - strand at 19.553 kb on + strand at 19.553 kb on + strand at 19.553 kb on + strand at 19.554 kb on - strand at 19.554 kb on - strand at 19.554 kb on - strand at 19.554 kb on - strand at 19.609 kb on + strand at 19.853 kb on + strand at 20.012 kb on + strand, within GFF1798 at 20.013 kb on - strand, within GFF1798 at 20.067 kb on + strand, within GFF1798 at 20.067 kb on + strand, within GFF1798 at 20.073 kb on + strand, within GFF1798 at 20.074 kb on - strand, within GFF1798 at 20.156 kb on + strand, within GFF1798 at 20.161 kb on + strand, within GFF1798 at 20.232 kb on + strand, within GFF1798 at 20.401 kb on - strand, within GFF1798 at 20.475 kb on + strand, within GFF1798 at 20.546 kb on + strand, within GFF1798 at 20.590 kb on + strand, within GFF1798 at 20.590 kb on + strand, within GFF1798 at 20.590 kb on + strand, within GFF1798 at 20.590 kb on + strand, within GFF1798 at 20.590 kb on + strand, within GFF1798 at 20.591 kb on - strand, within GFF1798 at 20.679 kb on - strand, within GFF1798 at 20.761 kb on - strand, within GFF1798 at 20.921 kb on - strand, within GFF1798 at 20.931 kb on - strand, within GFF1798 at 20.966 kb on - strand, within GFF1798 at 21.039 kb on + strand, within GFF1798 at 21.040 kb on - strand, within GFF1798 at 21.050 kb on + strand, within GFF1798 at 21.061 kb on + strand, within GFF1798 at 21.104 kb on - strand at 21.165 kb on - strand at 21.195 kb on - strand at 21.220 kb on - strand at 21.272 kb on - strand at 21.272 kb on - strand at 21.303 kb on + strand at 21.313 kb on + strand at 21.406 kb on - strand, within GFF1799 at 21.454 kb on + strand, within GFF1799 at 21.506 kb on - strand, within GFF1799 at 21.506 kb on - strand, within GFF1799 at 21.506 kb on - strand, within GFF1799 at 21.672 kb on + strand at 21.838 kb on + strand, within GFF1800 at 21.912 kb on + strand, within GFF1800 at 21.951 kb on + strand, within GFF1800
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 18,879 + GFF1797 0.28 +0.3 18,881 + GFF1797 0.28 +0.3 18,882 - GFF1797 0.28 +0.2 19,003 - GFF1797 0.41 +0.5 19,062 + GFF1797 0.47 -0.2 19,072 - GFF1797 0.48 -0.1 19,127 + GFF1797 0.54 +0.1 19,128 - GFF1797 0.54 +0.5 19,128 - GFF1797 0.54 -0.0 19,409 + GFF1797 0.84 +0.7 19,409 + GFF1797 0.84 +0.6 19,489 - +0.4 19,553 + +0.8 19,553 + -0.1 19,553 + +0.8 19,554 - -0.2 19,554 - +0.4 19,554 - +0.6 19,554 - +0.5 19,609 + +1.2 19,853 + -1.2 20,012 + GFF1798 0.11 -0.0 20,013 - GFF1798 0.11 +0.2 20,067 + GFF1798 0.15 +1.1 20,067 + GFF1798 0.15 -1.3 20,073 + GFF1798 0.16 +0.2 20,074 - GFF1798 0.16 -0.0 20,156 + GFF1798 0.22 -1.2 20,161 + GFF1798 0.22 +0.2 20,232 + GFF1798 0.27 -0.1 20,401 - GFF1798 0.39 +0.2 20,475 + GFF1798 0.45 +0.3 20,546 + GFF1798 0.50 +1.9 20,590 + GFF1798 0.53 -2.5 20,590 + GFF1798 0.53 +0.1 20,590 + GFF1798 0.53 +0.9 20,590 + GFF1798 0.53 -0.2 20,590 + GFF1798 0.53 +0.1 20,591 - GFF1798 0.53 -0.7 20,679 - GFF1798 0.59 -0.9 20,761 - GFF1798 0.65 -0.2 20,921 - GFF1798 0.77 -0.8 20,931 - GFF1798 0.78 +0.9 20,966 - GFF1798 0.80 +0.2 21,039 + GFF1798 0.85 +1.2 21,040 - GFF1798 0.85 +0.8 21,050 + GFF1798 0.86 +0.4 21,061 + GFF1798 0.87 +0.6 21,104 - -0.6 21,165 - -0.5 21,195 - -0.1 21,220 - +0.1 21,272 - -0.2 21,272 - -0.0 21,303 + +0.0 21,313 + -0.1 21,406 - GFF1799 0.32 +0.7 21,454 + GFF1799 0.46 -0.4 21,506 - GFF1799 0.62 -1.5 21,506 - GFF1799 0.62 -0.6 21,506 - GFF1799 0.62 -0.2 21,672 + +0.7 21,838 + GFF1800 0.10 +0.6 21,912 + GFF1800 0.20 +0.6 21,951 + GFF1800 0.26 +0.4
Or see this region's nucleotide sequence