Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1796 and GFF1797 are separated by 526 nucleotides GFF1797 and GFF1798 are separated by 298 nucleotides
GFF1796 - NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2), at 16,559 to 18,088
GFF1796
GFF1797 - Protein ydgH precursor, at 18,615 to 19,559
GFF1797
GFF1798 - Arginine/ornithine antiporter ArcD, at 19,858 to 21,240
GFF1798
Position (kb) 18
19
20 Strain fitness (log2 ratio) -1
0
1
2 at 17.660 kb on + strand, within GFF1796 at 17.700 kb on - strand, within GFF1796 at 17.765 kb on - strand, within GFF1796 at 17.872 kb on + strand, within GFF1796 at 18.088 kb on + strand at 18.393 kb on - strand at 18.463 kb on + strand at 18.476 kb on + strand at 18.571 kb on - strand at 18.571 kb on - strand at 18.724 kb on - strand, within GFF1797 at 18.724 kb on - strand, within GFF1797 at 18.752 kb on + strand, within GFF1797 at 18.819 kb on + strand, within GFF1797 at 18.819 kb on + strand, within GFF1797 at 18.820 kb on - strand, within GFF1797 at 18.879 kb on + strand, within GFF1797 at 18.881 kb on + strand, within GFF1797 at 18.882 kb on - strand, within GFF1797 at 19.003 kb on - strand, within GFF1797 at 19.062 kb on + strand, within GFF1797 at 19.072 kb on - strand, within GFF1797 at 19.127 kb on + strand, within GFF1797 at 19.128 kb on - strand, within GFF1797 at 19.128 kb on - strand, within GFF1797 at 19.409 kb on + strand, within GFF1797 at 19.409 kb on + strand, within GFF1797 at 19.489 kb on - strand at 19.553 kb on + strand at 19.553 kb on + strand at 19.553 kb on + strand at 19.554 kb on - strand at 19.554 kb on - strand at 19.554 kb on - strand at 19.554 kb on - strand at 19.609 kb on + strand at 19.853 kb on + strand at 20.012 kb on + strand, within GFF1798 at 20.013 kb on - strand, within GFF1798 at 20.067 kb on + strand, within GFF1798 at 20.067 kb on + strand, within GFF1798 at 20.073 kb on + strand, within GFF1798 at 20.074 kb on - strand, within GFF1798 at 20.156 kb on + strand, within GFF1798 at 20.161 kb on + strand, within GFF1798 at 20.232 kb on + strand, within GFF1798 at 20.401 kb on - strand, within GFF1798 at 20.475 kb on + strand, within GFF1798 at 20.546 kb on + strand, within GFF1798
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 17,660 + GFF1796 0.72 +0.6 17,700 - GFF1796 0.75 +0.8 17,765 - GFF1796 0.79 -0.3 17,872 + GFF1796 0.86 -0.4 18,088 + +0.2 18,393 - +0.0 18,463 + +0.0 18,476 + -0.2 18,571 - +0.8 18,571 - +0.5 18,724 - GFF1797 0.12 +0.0 18,724 - GFF1797 0.12 -0.3 18,752 + GFF1797 0.14 -0.4 18,819 + GFF1797 0.22 -0.7 18,819 + GFF1797 0.22 +0.6 18,820 - GFF1797 0.22 +0.5 18,879 + GFF1797 0.28 +0.3 18,881 + GFF1797 0.28 +0.3 18,882 - GFF1797 0.28 +0.2 19,003 - GFF1797 0.41 +0.5 19,062 + GFF1797 0.47 -0.2 19,072 - GFF1797 0.48 -0.1 19,127 + GFF1797 0.54 +0.1 19,128 - GFF1797 0.54 +0.5 19,128 - GFF1797 0.54 -0.0 19,409 + GFF1797 0.84 +0.7 19,409 + GFF1797 0.84 +0.6 19,489 - +0.4 19,553 + +0.8 19,553 + -0.1 19,553 + +0.8 19,554 - -0.2 19,554 - +0.4 19,554 - +0.6 19,554 - +0.5 19,609 + +1.2 19,853 + -1.2 20,012 + GFF1798 0.11 -0.0 20,013 - GFF1798 0.11 +0.2 20,067 + GFF1798 0.15 +1.1 20,067 + GFF1798 0.15 -1.3 20,073 + GFF1798 0.16 +0.2 20,074 - GFF1798 0.16 -0.0 20,156 + GFF1798 0.22 -1.2 20,161 + GFF1798 0.22 +0.2 20,232 + GFF1798 0.27 -0.1 20,401 - GFF1798 0.39 +0.2 20,475 + GFF1798 0.45 +0.3 20,546 + GFF1798 0.50 +1.9
Or see this region's nucleotide sequence