Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1777 and GFF1778 are separated by 42 nucleotides GFF1778 and GFF1779 are separated by 304 nucleotides
GFF1777 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.99.-), at 25 to 852
GFF1777
GFF1778 - Putative oxidoreductase component of anaerobic dehydrogenases; Chaperone protein TorD, at 895 to 1,509
GFF1778
GFF1779 - Putative ABC transporter membrane protein, at 1,814 to 2,524
GFF1779
Position (kb) 0
1
2 Strain fitness (log2 ratio) -1
0
1 at 0.415 kb on - strand, within GFF1777 at 0.431 kb on - strand, within GFF1777 at 0.714 kb on - strand, within GFF1777 at 0.822 kb on + strand at 0.823 kb on - strand at 0.823 kb on - strand at 0.830 kb on + strand at 0.830 kb on + strand at 1.007 kb on + strand, within GFF1778 at 1.007 kb on + strand, within GFF1778 at 1.007 kb on + strand, within GFF1778 at 1.007 kb on + strand, within GFF1778 at 1.074 kb on + strand, within GFF1778 at 1.122 kb on + strand, within GFF1778 at 1.184 kb on + strand, within GFF1778 at 1.185 kb on - strand, within GFF1778 at 1.200 kb on + strand, within GFF1778 at 1.200 kb on + strand, within GFF1778 at 1.201 kb on - strand, within GFF1778 at 1.201 kb on - strand, within GFF1778 at 1.634 kb on + strand at 1.658 kb on - strand at 1.675 kb on + strand at 1.724 kb on + strand at 1.724 kb on + strand at 1.725 kb on - strand at 1.726 kb on + strand at 1.727 kb on - strand at 1.839 kb on + strand at 1.882 kb on + strand at 2.189 kb on - strand, within GFF1779 at 2.192 kb on + strand, within GFF1779 at 2.277 kb on + strand, within GFF1779 at 2.283 kb on + strand, within GFF1779 at 2.284 kb on - strand, within GFF1779 at 2.284 kb on - strand, within GFF1779
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 415 - GFF1777 0.47 -0.1 431 - GFF1777 0.49 +0.2 714 - GFF1777 0.83 -1.2 822 + +0.6 823 - +0.4 823 - +0.9 830 + +0.5 830 + +0.1 1,007 + GFF1778 0.18 +0.5 1,007 + GFF1778 0.18 +0.2 1,007 + GFF1778 0.18 -0.2 1,007 + GFF1778 0.18 +0.4 1,074 + GFF1778 0.29 +0.2 1,122 + GFF1778 0.37 +0.3 1,184 + GFF1778 0.47 +0.3 1,185 - GFF1778 0.47 +0.6 1,200 + GFF1778 0.50 -1.1 1,200 + GFF1778 0.50 +0.3 1,201 - GFF1778 0.50 +0.2 1,201 - GFF1778 0.50 +0.4 1,634 + -0.7 1,658 - +0.0 1,675 + +0.2 1,724 + +0.8 1,724 + +0.5 1,725 - -0.0 1,726 + +0.3 1,727 - +0.2 1,839 + +0.5 1,882 + +0.4 2,189 - GFF1779 0.53 +0.6 2,192 + GFF1779 0.53 +0.0 2,277 + GFF1779 0.65 +0.3 2,283 + GFF1779 0.66 -0.6 2,284 - GFF1779 0.66 +1.1 2,284 - GFF1779 0.66 +0.2
Or see this region's nucleotide sequence