Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1755 and GFF1756 are separated by 147 nucleotides GFF1756 and GFF1757 are separated by 28 nucleotides
GFF1755 - DNA polymerase III beta subunit (EC 2.7.7.7), at 56,220 to 57,320
GFF1755
GFF1756 - DNA recombination and repair protein RecF, at 57,468 to 58,541
GFF1756
GFF1757 - DNA gyrase subunit B (EC 5.99.1.3), at 58,570 to 60,984
GFF1757
Position (kb) 57
58
59 Strain fitness (log2 ratio) -2
-1
0
1 at 57.319 kb on - strand at 57.341 kb on + strand at 57.383 kb on + strand at 57.384 kb on - strand at 57.393 kb on - strand at 57.463 kb on - strand at 57.463 kb on - strand at 57.465 kb on + strand at 57.465 kb on + strand at 57.465 kb on + strand at 57.465 kb on + strand at 57.465 kb on + strand at 57.466 kb on - strand at 57.466 kb on - strand at 57.467 kb on + strand at 57.467 kb on + strand at 57.467 kb on + strand at 57.467 kb on + strand at 57.467 kb on + strand at 57.467 kb on + strand at 57.467 kb on + strand at 57.467 kb on + strand at 57.468 kb on - strand at 57.468 kb on - strand at 57.593 kb on + strand, within GFF1756 at 57.593 kb on + strand, within GFF1756 at 57.593 kb on + strand, within GFF1756 at 57.597 kb on + strand, within GFF1756 at 57.597 kb on + strand, within GFF1756 at 57.598 kb on - strand, within GFF1756 at 57.598 kb on - strand, within GFF1756 at 57.688 kb on + strand, within GFF1756 at 57.731 kb on + strand, within GFF1756 at 57.731 kb on + strand, within GFF1756 at 57.732 kb on - strand, within GFF1756 at 57.833 kb on + strand, within GFF1756 at 57.833 kb on + strand, within GFF1756 at 58.155 kb on + strand, within GFF1756 at 58.156 kb on - strand, within GFF1756 at 58.193 kb on + strand, within GFF1756 at 58.204 kb on - strand, within GFF1756 at 58.363 kb on - strand, within GFF1756 at 58.426 kb on + strand, within GFF1756 at 58.463 kb on + strand at 58.478 kb on + strand at 58.480 kb on + strand at 58.481 kb on - strand at 58.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 57,319 - +0.6 57,341 + +0.1 57,383 + +0.2 57,384 - -0.1 57,393 - -0.7 57,463 - -2.2 57,463 - -0.8 57,465 + -0.1 57,465 + +0.0 57,465 + -0.1 57,465 + +0.1 57,465 + +0.1 57,466 - -0.7 57,466 - -0.1 57,467 + +0.2 57,467 + +0.1 57,467 + +0.7 57,467 + -0.3 57,467 + -0.6 57,467 + +0.5 57,467 + +0.1 57,467 + +0.6 57,468 - -1.2 57,468 - -0.8 57,593 + GFF1756 0.12 -1.5 57,593 + GFF1756 0.12 -1.0 57,593 + GFF1756 0.12 -0.4 57,597 + GFF1756 0.12 -1.0 57,597 + GFF1756 0.12 -0.5 57,598 - GFF1756 0.12 +0.2 57,598 - GFF1756 0.12 -0.1 57,688 + GFF1756 0.20 -1.8 57,731 + GFF1756 0.24 -1.5 57,731 + GFF1756 0.24 +0.1 57,732 - GFF1756 0.25 -0.7 57,833 + GFF1756 0.34 -1.6 57,833 + GFF1756 0.34 -1.0 58,155 + GFF1756 0.64 -0.5 58,156 - GFF1756 0.64 -0.6 58,193 + GFF1756 0.68 -1.0 58,204 - GFF1756 0.69 -1.5 58,363 - GFF1756 0.83 -1.4 58,426 + GFF1756 0.89 -0.7 58,463 + +0.7 58,478 + -1.7 58,480 + +0.3 58,481 - -0.1 58,481 - -1.3
Or see this region's nucleotide sequence