Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1731 and GFF1732 are separated by 61 nucleotides GFF1732 and GFF1733 are separated by 48 nucleotides GFF1733 and GFF1734 are separated by 166 nucleotides
GFF1731 - SgrR, sugar-phosphate stress, transcriptional activator of SgrS small RNA, at 30,955 to 32,745
GFF1731
GFF1732 - Shikimate 5-dehydrogenase I gamma (EC 1.1.1.25), at 32,807 to 33,625
GFF1732
GFF1733 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 33,674 to 35,047
GFF1733
GFF1734 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 35,214 to 36,254
GFF1734
Position (kb) 33
34
35
36 Strain fitness (log2 ratio) -2
-1
0
1 at 32.674 kb on + strand at 32.680 kb on + strand at 32.680 kb on + strand at 32.681 kb on - strand at 32.891 kb on + strand, within GFF1732 at 32.891 kb on + strand, within GFF1732 at 32.919 kb on - strand, within GFF1732 at 32.919 kb on - strand, within GFF1732 at 32.925 kb on - strand, within GFF1732 at 33.077 kb on - strand, within GFF1732 at 33.077 kb on - strand, within GFF1732 at 33.077 kb on - strand, within GFF1732 at 33.091 kb on + strand, within GFF1732 at 33.125 kb on + strand, within GFF1732 at 33.156 kb on - strand, within GFF1732 at 33.203 kb on - strand, within GFF1732 at 33.257 kb on - strand, within GFF1732 at 33.408 kb on - strand, within GFF1732 at 33.439 kb on + strand, within GFF1732 at 33.536 kb on - strand, within GFF1732 at 33.536 kb on - strand, within GFF1732 at 33.563 kb on + strand at 33.673 kb on + strand at 33.731 kb on - strand at 33.912 kb on - strand, within GFF1733 at 33.987 kb on - strand, within GFF1733 at 34.045 kb on + strand, within GFF1733 at 34.046 kb on - strand, within GFF1733 at 34.054 kb on - strand, within GFF1733 at 34.072 kb on + strand, within GFF1733 at 34.171 kb on - strand, within GFF1733 at 34.186 kb on + strand, within GFF1733 at 34.187 kb on - strand, within GFF1733 at 34.187 kb on - strand, within GFF1733 at 34.229 kb on - strand, within GFF1733 at 34.402 kb on + strand, within GFF1733 at 34.449 kb on + strand, within GFF1733 at 34.449 kb on + strand, within GFF1733 at 34.537 kb on + strand, within GFF1733 at 34.537 kb on + strand, within GFF1733 at 34.598 kb on - strand, within GFF1733 at 34.610 kb on - strand, within GFF1733 at 34.626 kb on + strand, within GFF1733 at 34.643 kb on + strand, within GFF1733 at 34.643 kb on + strand, within GFF1733 at 34.644 kb on - strand, within GFF1733 at 34.880 kb on + strand, within GFF1733 at 34.881 kb on - strand, within GFF1733 at 35.006 kb on + strand at 35.134 kb on + strand at 35.134 kb on + strand at 35.134 kb on + strand at 35.134 kb on + strand at 35.136 kb on + strand at 35.136 kb on + strand at 35.136 kb on + strand at 35.136 kb on + strand at 35.137 kb on - strand at 35.137 kb on - strand at 35.137 kb on - strand at 35.170 kb on + strand at 35.170 kb on + strand at 35.213 kb on + strand at 35.213 kb on + strand at 35.730 kb on + strand, within GFF1734 at 35.780 kb on + strand, within GFF1734 at 35.839 kb on + strand, within GFF1734
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 32,674 + -0.3 32,680 + +0.6 32,680 + -0.0 32,681 - -0.0 32,891 + GFF1732 0.10 +0.2 32,891 + GFF1732 0.10 -0.0 32,919 - GFF1732 0.14 +0.2 32,919 - GFF1732 0.14 +0.0 32,925 - GFF1732 0.14 -0.1 33,077 - GFF1732 0.33 +0.0 33,077 - GFF1732 0.33 +0.3 33,077 - GFF1732 0.33 -0.1 33,091 + GFF1732 0.35 -0.4 33,125 + GFF1732 0.39 +0.9 33,156 - GFF1732 0.43 +0.3 33,203 - GFF1732 0.48 -0.2 33,257 - GFF1732 0.55 -0.4 33,408 - GFF1732 0.73 +0.1 33,439 + GFF1732 0.77 +0.6 33,536 - GFF1732 0.89 -0.3 33,536 - GFF1732 0.89 -0.1 33,563 + +0.3 33,673 + -0.1 33,731 - -0.2 33,912 - GFF1733 0.17 -0.0 33,987 - GFF1733 0.23 -0.1 34,045 + GFF1733 0.27 +0.5 34,046 - GFF1733 0.27 +0.1 34,054 - GFF1733 0.28 +0.5 34,072 + GFF1733 0.29 +0.1 34,171 - GFF1733 0.36 -0.7 34,186 + GFF1733 0.37 +0.3 34,187 - GFF1733 0.37 +0.5 34,187 - GFF1733 0.37 +0.2 34,229 - GFF1733 0.40 +0.4 34,402 + GFF1733 0.53 +0.1 34,449 + GFF1733 0.56 +0.8 34,449 + GFF1733 0.56 +0.0 34,537 + GFF1733 0.63 -0.6 34,537 + GFF1733 0.63 +0.4 34,598 - GFF1733 0.67 +0.1 34,610 - GFF1733 0.68 -0.1 34,626 + GFF1733 0.69 -0.4 34,643 + GFF1733 0.71 -0.3 34,643 + GFF1733 0.71 +0.3 34,644 - GFF1733 0.71 +0.2 34,880 + GFF1733 0.88 -0.4 34,881 - GFF1733 0.88 +0.0 35,006 + +0.3 35,134 + +0.5 35,134 + -1.8 35,134 + +0.2 35,134 + +0.6 35,136 + +0.3 35,136 + -0.3 35,136 + -0.1 35,136 + +0.4 35,137 - -1.1 35,137 - +0.2 35,137 - -0.1 35,170 + +0.5 35,170 + -0.0 35,213 + -1.2 35,213 + +0.3 35,730 + GFF1734 0.50 -0.9 35,780 + GFF1734 0.54 +0.6 35,839 + GFF1734 0.60 -1.2
Or see this region's nucleotide sequence