Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1630 and GFF1631 are separated by 234 nucleotides GFF1631 and GFF1632 are separated by 341 nucleotides
GFF1630 - Phosphogluconate dehydratase (EC 4.2.1.12), at 14,042 to 15,853
GFF1630
GFF1631 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 16,088 to 17,563
GFF1631
GFF1632 - Phosphogluconate repressor HexR, RpiR family, at 17,905 to 18,774
GFF1632
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-1
0
1 at 15.210 kb on + strand, within GFF1630 at 15.267 kb on - strand, within GFF1630 at 15.425 kb on - strand, within GFF1630 at 15.441 kb on - strand, within GFF1630 at 15.584 kb on - strand, within GFF1630 at 15.667 kb on + strand, within GFF1630 at 15.667 kb on + strand, within GFF1630 at 15.787 kb on - strand at 16.379 kb on - strand, within GFF1631 at 16.395 kb on - strand, within GFF1631 at 16.415 kb on - strand, within GFF1631 at 16.564 kb on - strand, within GFF1631 at 16.966 kb on + strand, within GFF1631 at 16.977 kb on - strand, within GFF1631 at 17.046 kb on + strand, within GFF1631 at 17.049 kb on - strand, within GFF1631 at 17.391 kb on + strand, within GFF1631 at 17.391 kb on + strand, within GFF1631 at 17.580 kb on - strand at 17.591 kb on - strand at 17.612 kb on + strand at 17.633 kb on + strand at 17.701 kb on + strand at 17.702 kb on - strand at 17.702 kb on - strand at 17.702 kb on - strand at 17.702 kb on - strand at 17.703 kb on + strand at 17.704 kb on - strand at 17.776 kb on + strand at 17.836 kb on - strand at 17.838 kb on + strand at 17.910 kb on + strand at 18.008 kb on + strand, within GFF1632 at 18.142 kb on + strand, within GFF1632 at 18.143 kb on - strand, within GFF1632 at 18.184 kb on + strand, within GFF1632 at 18.186 kb on + strand, within GFF1632 at 18.187 kb on - strand, within GFF1632 at 18.275 kb on + strand, within GFF1632 at 18.348 kb on + strand, within GFF1632 at 18.415 kb on - strand, within GFF1632
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 15,210 + GFF1630 0.64 +0.3 15,267 - GFF1630 0.68 +0.2 15,425 - GFF1630 0.76 +0.3 15,441 - GFF1630 0.77 +0.5 15,584 - GFF1630 0.85 +0.6 15,667 + GFF1630 0.90 -0.6 15,667 + GFF1630 0.90 -0.6 15,787 - +0.5 16,379 - GFF1631 0.20 +1.1 16,395 - GFF1631 0.21 +0.4 16,415 - GFF1631 0.22 +0.4 16,564 - GFF1631 0.32 +0.8 16,966 + GFF1631 0.59 +0.3 16,977 - GFF1631 0.60 +0.9 17,046 + GFF1631 0.65 +0.5 17,049 - GFF1631 0.65 +0.2 17,391 + GFF1631 0.88 +0.3 17,391 + GFF1631 0.88 +0.9 17,580 - +0.5 17,591 - -0.5 17,612 + +0.6 17,633 + +0.1 17,701 + +0.5 17,702 - -0.0 17,702 - +0.2 17,702 - -0.0 17,702 - +0.4 17,703 + -0.3 17,704 - -0.7 17,776 + +0.2 17,836 - +0.1 17,838 + +0.3 17,910 + +0.3 18,008 + GFF1632 0.12 +0.1 18,142 + GFF1632 0.27 -0.6 18,143 - GFF1632 0.27 -0.7 18,184 + GFF1632 0.32 -0.8 18,186 + GFF1632 0.32 +0.0 18,187 - GFF1632 0.32 -0.6 18,275 + GFF1632 0.43 -1.2 18,348 + GFF1632 0.51 -0.1 18,415 - GFF1632 0.59 -0.6
Or see this region's nucleotide sequence