Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1624 and GFF1625 are separated by 212 nucleotides GFF1625 and GFF1626 are separated by 93 nucleotides GFF1626 and GFF1627 are separated by 67 nucleotides GFF1627 and GFF1628 are separated by 130 nucleotides
GFF1624 - Protease II (EC 3.4.21.83), at 8,226 to 10,277
GFF1624
GFF1625 - FIG004088: inner membrane protein YebE, at 10,490 to 11,149
GFF1625
GFF1626 - FIG01200701: possible membrane protein, at 11,243 to 11,596
GFF1626
GFF1627 - DNA damage-inducible gene in SOS regulon, dependent on cyclic AMP and H-NS, at 11,664 to 11,954
GFF1627
GFF1628 - Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-), at 12,085 to 13,263
GFF1628
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-1
0
1
2 at 9.529 kb on - strand, within GFF1624 at 9.572 kb on - strand, within GFF1624 at 9.572 kb on - strand, within GFF1624 at 9.605 kb on - strand, within GFF1624 at 9.630 kb on + strand, within GFF1624 at 9.630 kb on + strand, within GFF1624 at 9.702 kb on - strand, within GFF1624 at 9.985 kb on + strand, within GFF1624 at 9.986 kb on - strand, within GFF1624 at 9.986 kb on - strand, within GFF1624 at 9.986 kb on - strand, within GFF1624 at 9.986 kb on - strand, within GFF1624 at 9.995 kb on - strand, within GFF1624 at 10.012 kb on + strand, within GFF1624 at 10.012 kb on + strand, within GFF1624 at 10.016 kb on + strand, within GFF1624 at 10.016 kb on + strand, within GFF1624 at 10.017 kb on - strand, within GFF1624 at 10.017 kb on - strand, within GFF1624 at 10.017 kb on - strand, within GFF1624 at 10.017 kb on - strand, within GFF1624 at 10.018 kb on + strand, within GFF1624 at 10.018 kb on + strand, within GFF1624 at 10.018 kb on + strand, within GFF1624 at 10.018 kb on + strand, within GFF1624 at 10.107 kb on + strand at 10.211 kb on - strand at 10.214 kb on + strand at 10.215 kb on - strand at 10.298 kb on - strand at 10.343 kb on + strand at 10.344 kb on - strand at 10.416 kb on + strand at 10.421 kb on + strand at 10.422 kb on - strand at 10.430 kb on + strand at 10.474 kb on - strand at 10.587 kb on + strand, within GFF1625 at 10.591 kb on + strand, within GFF1625 at 10.613 kb on - strand, within GFF1625 at 10.795 kb on + strand, within GFF1625 at 10.795 kb on + strand, within GFF1625 at 10.908 kb on - strand, within GFF1625 at 10.912 kb on + strand, within GFF1625 at 10.920 kb on + strand, within GFF1625 at 10.984 kb on - strand, within GFF1625 at 11.012 kb on - strand, within GFF1625 at 11.163 kb on + strand at 11.163 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.762 kb on + strand, within GFF1627 at 11.763 kb on - strand, within GFF1627 at 11.763 kb on - strand, within GFF1627 at 11.936 kb on - strand at 11.936 kb on - strand at 11.936 kb on - strand at 11.936 kb on - strand at 11.936 kb on - strand at 12.071 kb on - strand at 12.071 kb on - strand at 12.093 kb on - strand at 12.134 kb on + strand at 12.134 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 9,529 - GFF1624 0.63 -0.0 9,572 - GFF1624 0.66 -0.5 9,572 - GFF1624 0.66 +0.4 9,605 - GFF1624 0.67 +0.1 9,630 + GFF1624 0.68 -1.1 9,630 + GFF1624 0.68 -0.3 9,702 - GFF1624 0.72 +0.6 9,985 + GFF1624 0.86 +0.1 9,986 - GFF1624 0.86 -0.4 9,986 - GFF1624 0.86 -0.2 9,986 - GFF1624 0.86 +0.3 9,986 - GFF1624 0.86 +0.3 9,995 - GFF1624 0.86 -0.2 10,012 + GFF1624 0.87 -0.5 10,012 + GFF1624 0.87 +0.4 10,016 + GFF1624 0.87 +0.9 10,016 + GFF1624 0.87 +0.4 10,017 - GFF1624 0.87 +0.0 10,017 - GFF1624 0.87 -0.0 10,017 - GFF1624 0.87 +0.3 10,017 - GFF1624 0.87 -0.0 10,018 + GFF1624 0.87 -0.2 10,018 + GFF1624 0.87 -0.1 10,018 + GFF1624 0.87 -1.0 10,018 + GFF1624 0.87 -0.2 10,107 + +0.5 10,211 - +0.4 10,214 + +0.3 10,215 - +0.2 10,298 - +0.7 10,343 + +0.0 10,344 - +0.2 10,416 + +0.3 10,421 + +0.2 10,422 - +1.5 10,430 + +0.0 10,474 - +1.0 10,587 + GFF1625 0.15 +0.4 10,591 + GFF1625 0.15 -0.0 10,613 - GFF1625 0.19 -0.2 10,795 + GFF1625 0.46 -0.4 10,795 + GFF1625 0.46 +1.8 10,908 - GFF1625 0.63 +0.7 10,912 + GFF1625 0.64 +0.2 10,920 + GFF1625 0.65 -0.1 10,984 - GFF1625 0.75 +0.3 11,012 - GFF1625 0.79 +0.5 11,163 + +0.6 11,163 + -0.7 11,254 + +0.2 11,254 + -0.4 11,762 + GFF1627 0.34 +0.5 11,763 - GFF1627 0.34 -0.5 11,763 - GFF1627 0.34 -0.6 11,936 - +0.8 11,936 - +1.2 11,936 - +0.2 11,936 - -1.1 11,936 - -0.2 12,071 - -1.1 12,071 - -0.4 12,093 - +0.3 12,134 + +1.0 12,134 + +0.0
Or see this region's nucleotide sequence