Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1556 and GFF1557 are separated by 132 nucleotides GFF1557 and GFF1558 overlap by 14 nucleotides GFF1558 and GFF1559 are separated by 38 nucleotides GFF1559 and GFF1560 overlap by 4 nucleotides
GFF1556 - HoxN/HupN/NixA family nickel/cobalt transporter, at 19,395 to 20,408
GFF1556
GFF1557 - Tricarboxylate transport sensor protein TctE, at 20,541 to 21,956
GFF1557
GFF1558 - Tricarboxylate transport transcriptional regulator TctD, at 21,943 to 22,617
GFF1558
GFF1559 - hypothetical protein, at 22,656 to 22,775
GFF1559
GFF1560 - Tricarboxylate transport protein TctC, at 22,772 to 23,749
GFF1560
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1 at 19.636 kb on + strand, within GFF1556 at 19.636 kb on + strand, within GFF1556 at 19.639 kb on + strand, within GFF1556 at 19.639 kb on + strand, within GFF1556 at 19.640 kb on - strand, within GFF1556 at 19.646 kb on + strand, within GFF1556 at 19.651 kb on - strand, within GFF1556 at 19.767 kb on + strand, within GFF1556 at 19.778 kb on + strand, within GFF1556 at 19.779 kb on - strand, within GFF1556 at 19.858 kb on + strand, within GFF1556 at 19.859 kb on - strand, within GFF1556 at 20.087 kb on + strand, within GFF1556 at 20.087 kb on + strand, within GFF1556 at 20.087 kb on + strand, within GFF1556 at 20.274 kb on - strand, within GFF1556 at 20.275 kb on + strand, within GFF1556 at 20.351 kb on + strand at 20.394 kb on - strand at 20.394 kb on - strand at 20.402 kb on + strand at 20.403 kb on - strand at 20.559 kb on + strand at 20.560 kb on - strand at 20.578 kb on + strand at 20.579 kb on - strand at 20.579 kb on - strand at 20.827 kb on + strand, within GFF1557 at 20.859 kb on + strand, within GFF1557 at 20.860 kb on - strand, within GFF1557 at 21.026 kb on + strand, within GFF1557 at 21.026 kb on + strand, within GFF1557 at 21.026 kb on + strand, within GFF1557 at 21.270 kb on + strand, within GFF1557 at 21.337 kb on - strand, within GFF1557 at 21.337 kb on - strand, within GFF1557 at 21.363 kb on - strand, within GFF1557 at 21.467 kb on - strand, within GFF1557 at 21.496 kb on + strand, within GFF1557 at 21.541 kb on - strand, within GFF1557 at 21.582 kb on - strand, within GFF1557 at 21.587 kb on - strand, within GFF1557 at 21.587 kb on - strand, within GFF1557 at 21.716 kb on + strand, within GFF1557 at 21.728 kb on + strand, within GFF1557 at 21.818 kb on + strand at 21.860 kb on - strand at 21.928 kb on + strand at 22.042 kb on - strand, within GFF1558 at 22.203 kb on - strand, within GFF1558 at 22.325 kb on + strand, within GFF1558 at 22.458 kb on + strand, within GFF1558 at 22.712 kb on + strand, within GFF1559 at 22.823 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 19,636 + GFF1556 0.24 -0.8 19,636 + GFF1556 0.24 -0.8 19,639 + GFF1556 0.24 +0.5 19,639 + GFF1556 0.24 -0.4 19,640 - GFF1556 0.24 +0.3 19,646 + GFF1556 0.25 +0.0 19,651 - GFF1556 0.25 -0.7 19,767 + GFF1556 0.37 -0.5 19,778 + GFF1556 0.38 +0.0 19,779 - GFF1556 0.38 -0.4 19,858 + GFF1556 0.46 -1.1 19,859 - GFF1556 0.46 -0.2 20,087 + GFF1556 0.68 +0.7 20,087 + GFF1556 0.68 +0.1 20,087 + GFF1556 0.68 +0.0 20,274 - GFF1556 0.87 +0.3 20,275 + GFF1556 0.87 +0.3 20,351 + -0.0 20,394 - +0.1 20,394 - +0.4 20,402 + +0.5 20,403 - -0.7 20,559 + -0.8 20,560 - +0.2 20,578 + +0.6 20,579 - -0.8 20,579 - +0.5 20,827 + GFF1557 0.20 +0.2 20,859 + GFF1557 0.22 -0.6 20,860 - GFF1557 0.23 +0.0 21,026 + GFF1557 0.34 -1.8 21,026 + GFF1557 0.34 -0.3 21,026 + GFF1557 0.34 +0.3 21,270 + GFF1557 0.51 +0.2 21,337 - GFF1557 0.56 -0.8 21,337 - GFF1557 0.56 +0.3 21,363 - GFF1557 0.58 +0.8 21,467 - GFF1557 0.65 +0.4 21,496 + GFF1557 0.67 -0.1 21,541 - GFF1557 0.71 -0.1 21,582 - GFF1557 0.74 -0.3 21,587 - GFF1557 0.74 +0.1 21,587 - GFF1557 0.74 -0.6 21,716 + GFF1557 0.83 -0.4 21,728 + GFF1557 0.84 +0.5 21,818 + +0.5 21,860 - +0.7 21,928 + +0.1 22,042 - GFF1558 0.15 -0.2 22,203 - GFF1558 0.39 +1.3 22,325 + GFF1558 0.57 +0.3 22,458 + GFF1558 0.76 +0.0 22,712 + GFF1559 0.47 -0.7 22,823 - -0.8
Or see this region's nucleotide sequence