Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1536 and GFF1537 are separated by 97 nucleotides GFF1537 and GFF1538 are separated by 509 nucleotides GFF1538 and GFF1539 overlap by 4 nucleotides GFF1539 and GFF1540 overlap by 4 nucleotides
GFF1536 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 41,215 to 42,105
GFF1536
GFF1537 - Glycerate kinase (EC 2.7.1.31), at 42,203 to 43,348
GFF1537
GFF1538 - FIG01045316: hypothetical protein, at 43,858 to 43,977
GFF1538
GFF1539 - FIG01045316: hypothetical protein, at 43,974 to 44,093
GFF1539
GFF1540 - FIG01045316: hypothetical protein, at 44,090 to 44,209
GFF1540
Position (kb) 42
43
44 Strain fitness (log2 ratio) -1
0
1 at 41.243 kb on + strand at 41.244 kb on - strand at 41.244 kb on - strand at 41.416 kb on - strand, within GFF1536 at 41.416 kb on - strand, within GFF1536 at 41.458 kb on - strand, within GFF1536 at 41.496 kb on + strand, within GFF1536 at 41.497 kb on - strand, within GFF1536 at 41.656 kb on + strand, within GFF1536 at 41.657 kb on - strand, within GFF1536 at 41.660 kb on - strand, within GFF1536 at 41.931 kb on + strand, within GFF1536 at 41.932 kb on - strand, within GFF1536 at 41.956 kb on - strand, within GFF1536 at 42.067 kb on + strand at 42.197 kb on - strand at 42.230 kb on + strand at 42.233 kb on - strand at 42.308 kb on + strand at 42.308 kb on + strand at 42.309 kb on - strand at 42.526 kb on + strand, within GFF1537 at 42.788 kb on - strand, within GFF1537 at 42.888 kb on - strand, within GFF1537 at 42.996 kb on + strand, within GFF1537 at 43.090 kb on + strand, within GFF1537 at 43.151 kb on - strand, within GFF1537 at 43.197 kb on + strand, within GFF1537 at 43.197 kb on + strand, within GFF1537 at 43.198 kb on - strand, within GFF1537 at 43.200 kb on + strand, within GFF1537 at 43.200 kb on + strand, within GFF1537 at 43.200 kb on + strand, within GFF1537 at 43.200 kb on + strand, within GFF1537 at 43.201 kb on - strand, within GFF1537 at 43.201 kb on - strand, within GFF1537 at 43.201 kb on - strand, within GFF1537 at 43.201 kb on - strand, within GFF1537 at 43.286 kb on - strand at 43.941 kb on + strand, within GFF1538 at 44.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 41,243 + +0.6 41,244 - +0.4 41,244 - +1.7 41,416 - GFF1536 0.23 +0.2 41,416 - GFF1536 0.23 -0.2 41,458 - GFF1536 0.27 +0.5 41,496 + GFF1536 0.32 +0.4 41,497 - GFF1536 0.32 -0.2 41,656 + GFF1536 0.49 -1.7 41,657 - GFF1536 0.50 -0.2 41,660 - GFF1536 0.50 +0.1 41,931 + GFF1536 0.80 +0.8 41,932 - GFF1536 0.80 -0.3 41,956 - GFF1536 0.83 +0.4 42,067 + +0.0 42,197 - +0.7 42,230 + +0.8 42,233 - -0.2 42,308 + +0.4 42,308 + +0.7 42,309 - +0.1 42,526 + GFF1537 0.28 +0.5 42,788 - GFF1537 0.51 +0.5 42,888 - GFF1537 0.60 -0.4 42,996 + GFF1537 0.69 +0.0 43,090 + GFF1537 0.77 +0.1 43,151 - GFF1537 0.83 -0.4 43,197 + GFF1537 0.87 +0.5 43,197 + GFF1537 0.87 +0.8 43,198 - GFF1537 0.87 -0.3 43,200 + GFF1537 0.87 +0.3 43,200 + GFF1537 0.87 +0.4 43,200 + GFF1537 0.87 +0.5 43,200 + GFF1537 0.87 -0.6 43,201 - GFF1537 0.87 +0.9 43,201 - GFF1537 0.87 +0.1 43,201 - GFF1537 0.87 -0.0 43,201 - GFF1537 0.87 -0.6 43,286 - -0.3 43,941 + GFF1538 0.69 +0.2 44,233 - +0.1
Or see this region's nucleotide sequence