Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1385 and GFF1386 overlap by 41 nucleotides GFF1386 and GFF1387 are separated by 51 nucleotides GFF1387 and GFF1388 overlap by 1 nucleotides GFF1388 and GFF1389 overlap by 11 nucleotides GFF1389 and GFF1390 overlap by 4 nucleotides GFF1390 and GFF1391 are separated by 159 nucleotides
GFF1385 - hypothetical protein, at 33,113 to 33,241
GFF1385
GFF1386 - FIG00639134: hypothetical protein, at 33,201 to 33,518
GFF1386
GFF1387 - Putative phage tail core protein, at 33,570 to 34,094
GFF1387
GFF1388 - Phage tail sheath monomer, at 34,094 to 35,521
GFF1388
GFF1389 - FIG074102: hypothetical protein, at 35,511 to 35,708
GFF1389
GFF1390 - Phage protein, at 35,705 to 36,160
GFF1390
GFF1391 - Putative inner membrane protein, at 36,320 to 36,634
GFF1391
Position (kb)
34
35
36 Strain fitness (log2 ratio)
-2
-1
0
1 at 33.173 kb on - strand, within GFF1385 at 33.314 kb on - strand, within GFF1386 at 33.447 kb on + strand, within GFF1386 at 33.448 kb on - strand, within GFF1386 at 33.485 kb on + strand, within GFF1386 at 33.522 kb on - strand at 33.582 kb on - strand at 33.813 kb on - strand, within GFF1387 at 33.872 kb on + strand, within GFF1387 at 33.984 kb on - strand, within GFF1387 at 34.008 kb on - strand, within GFF1387 at 34.353 kb on + strand, within GFF1388 at 34.354 kb on - strand, within GFF1388 at 34.354 kb on - strand, within GFF1388 at 34.421 kb on - strand, within GFF1388 at 34.421 kb on - strand, within GFF1388 at 34.421 kb on - strand, within GFF1388 at 34.426 kb on + strand, within GFF1388 at 34.426 kb on + strand, within GFF1388 at 34.426 kb on + strand, within GFF1388 at 34.427 kb on - strand, within GFF1388 at 34.427 kb on - strand, within GFF1388 at 34.485 kb on + strand, within GFF1388 at 34.485 kb on + strand, within GFF1388 at 34.486 kb on - strand, within GFF1388 at 34.486 kb on - strand, within GFF1388 at 34.762 kb on + strand, within GFF1388 at 34.762 kb on + strand, within GFF1388 at 34.830 kb on + strand, within GFF1388 at 34.831 kb on - strand, within GFF1388 at 34.834 kb on + strand, within GFF1388 at 34.835 kb on - strand, within GFF1388 at 35.034 kb on + strand, within GFF1388 at 35.049 kb on + strand, within GFF1388 at 35.050 kb on - strand, within GFF1388 at 35.119 kb on - strand, within GFF1388 at 35.134 kb on + strand, within GFF1388 at 35.137 kb on - strand, within GFF1388 at 35.275 kb on + strand, within GFF1388 at 35.495 kb on + strand at 35.496 kb on - strand at 35.496 kb on - strand at 35.513 kb on - strand at 35.646 kb on + strand, within GFF1389 at 35.647 kb on - strand, within GFF1389 at 35.833 kb on - strand, within GFF1390 at 35.919 kb on - strand, within GFF1390 at 35.930 kb on + strand, within GFF1390 at 35.931 kb on - strand, within GFF1390 at 36.095 kb on - strand, within GFF1390 at 36.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 33,173 - GFF1385 0.47 +0.1 33,314 - GFF1386 0.36 +0.2 33,447 + GFF1386 0.77 -0.0 33,448 - GFF1386 0.78 +0.1 33,485 + GFF1386 0.89 -0.3 33,522 - -1.6 33,582 - +0.4 33,813 - GFF1387 0.46 -0.8 33,872 + GFF1387 0.58 -0.3 33,984 - GFF1387 0.79 +0.3 34,008 - GFF1387 0.83 +0.9 34,353 + GFF1388 0.18 +0.5 34,354 - GFF1388 0.18 -0.1 34,354 - GFF1388 0.18 -1.8 34,421 - GFF1388 0.23 -2.2 34,421 - GFF1388 0.23 -0.4 34,421 - GFF1388 0.23 +0.1 34,426 + GFF1388 0.23 -0.6 34,426 + GFF1388 0.23 -0.1 34,426 + GFF1388 0.23 +0.2 34,427 - GFF1388 0.23 +0.3 34,427 - GFF1388 0.23 +0.0 34,485 + GFF1388 0.27 +0.8 34,485 + GFF1388 0.27 -0.1 34,486 - GFF1388 0.27 -0.5 34,486 - GFF1388 0.27 -0.9 34,762 + GFF1388 0.47 +0.1 34,762 + GFF1388 0.47 -0.6 34,830 + GFF1388 0.52 +0.3 34,831 - GFF1388 0.52 +0.2 34,834 + GFF1388 0.52 -0.8 34,835 - GFF1388 0.52 +0.4 35,034 + GFF1388 0.66 -0.6 35,049 + GFF1388 0.67 +0.1 35,050 - GFF1388 0.67 -0.0 35,119 - GFF1388 0.72 -0.6 35,134 + GFF1388 0.73 +0.3 35,137 - GFF1388 0.73 +0.1 35,275 + GFF1388 0.83 -0.1 35,495 + -0.3 35,496 - -0.2 35,496 - -0.3 35,513 - +0.4 35,646 + GFF1389 0.68 -0.4 35,647 - GFF1389 0.69 -0.6 35,833 - GFF1390 0.28 -0.0 35,919 - GFF1390 0.47 +0.5 35,930 + GFF1390 0.49 -1.3 35,931 - GFF1390 0.50 +0.0 36,095 - GFF1390 0.86 +0.3 36,120 - -0.7
Or see this region's nucleotide sequence