Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1366 and GFF1367 are separated by 359 nucleotides GFF1367 and GFF1368 are separated by 75 nucleotides GFF1368 and GFF1369 overlap by 26 nucleotides GFF1369 and GFF1370 overlap by 4 nucleotides GFF1370 and GFF1371 are separated by 4 nucleotides
GFF1366 - putative cytoplasmic protein, at 18,046 to 18,774
GFF1366
GFF1367 - putative inner membrane protein, at 19,134 to 19,322
GFF1367
GFF1368 - hypothetical protein, at 19,398 to 19,535
GFF1368
GFF1369 - putative cytoplasmic protein, at 19,510 to 19,965
GFF1369
GFF1370 - putative cytoplasmic protein, at 19,962 to 20,567
GFF1370
GFF1371 - putative phage tail fiber protein H, at 20,572 to 22,317
GFF1371
Position (kb) 19
20 Strain fitness (log2 ratio) -1
0
1 at 18.549 kb on + strand, within GFF1366 at 18.708 kb on + strand at 18.835 kb on - strand at 19.580 kb on - strand, within GFF1369 at 19.621 kb on - strand, within GFF1369 at 19.640 kb on + strand, within GFF1369 at 19.673 kb on + strand, within GFF1369 at 19.749 kb on + strand, within GFF1369 at 19.766 kb on - strand, within GFF1369 at 19.766 kb on - strand, within GFF1369 at 19.768 kb on + strand, within GFF1369 at 19.768 kb on + strand, within GFF1369 at 19.769 kb on - strand, within GFF1369 at 19.769 kb on - strand, within GFF1369 at 19.817 kb on + strand, within GFF1369 at 19.860 kb on - strand, within GFF1369 at 19.932 kb on + strand at 19.934 kb on + strand at 19.935 kb on - strand at 19.991 kb on - strand at 20.067 kb on - strand, within GFF1370 at 20.079 kb on - strand, within GFF1370 at 20.217 kb on - strand, within GFF1370 at 20.253 kb on - strand, within GFF1370 at 20.311 kb on - strand, within GFF1370 at 20.549 kb on - strand at 20.567 kb on + strand at 20.633 kb on - strand at 20.633 kb on - strand at 20.634 kb on + strand at 20.635 kb on - strand at 20.635 kb on - strand at 20.739 kb on + strand at 20.763 kb on + strand, within GFF1371 at 20.932 kb on - strand, within GFF1371
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 18,549 + GFF1366 0.69 -1.1 18,708 + -0.5 18,835 - -0.1 19,580 - GFF1369 0.15 +0.3 19,621 - GFF1369 0.24 -0.0 19,640 + GFF1369 0.29 +1.2 19,673 + GFF1369 0.36 +0.0 19,749 + GFF1369 0.52 +0.4 19,766 - GFF1369 0.56 -0.3 19,766 - GFF1369 0.56 +0.0 19,768 + GFF1369 0.57 +0.1 19,768 + GFF1369 0.57 +0.2 19,769 - GFF1369 0.57 +0.4 19,769 - GFF1369 0.57 -0.0 19,817 + GFF1369 0.67 +0.7 19,860 - GFF1369 0.77 -0.2 19,932 + -0.0 19,934 + -0.6 19,935 - -0.1 19,991 - -0.6 20,067 - GFF1370 0.17 +1.4 20,079 - GFF1370 0.19 -0.0 20,217 - GFF1370 0.42 +0.3 20,253 - GFF1370 0.48 +0.2 20,311 - GFF1370 0.58 -0.3 20,549 - +0.0 20,567 + -0.0 20,633 - +0.1 20,633 - -0.4 20,634 + +1.3 20,635 - +0.2 20,635 - +0.2 20,739 + +0.2 20,763 + GFF1371 0.11 -0.0 20,932 - GFF1371 0.21 +0.2
Or see this region's nucleotide sequence