Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF133 and GFF134 are separated by 30 nucleotides GFF134 and GFF135 are separated by 225 nucleotides GFF135 and GFF136 are separated by 41 nucleotides
GFF133 - Type IV fimbrial assembly protein PilC, at 122,761 to 123,963
GFF133
GFF134 - GMP reductase (EC 1.7.1.7), at 123,994 to 125,037
GFF134
GFF135 - Dephospho-CoA kinase (EC 2.7.1.24), at 125,263 to 125,883
GFF135
GFF136 - FIG002842: hypothetical protein, at 125,925 to 126,626
GFF136
Position (kb)
123
124
125
126 Strain fitness (log2 ratio)
-1
0
1 at 123.462 kb on - strand, within GFF133 at 123.627 kb on + strand, within GFF133 at 123.730 kb on - strand, within GFF133 at 123.783 kb on + strand, within GFF133 at 123.795 kb on + strand, within GFF133 at 123.887 kb on + strand at 123.888 kb on - strand at 123.891 kb on + strand at 123.917 kb on - strand at 123.917 kb on - strand at 123.917 kb on - strand at 123.917 kb on - strand at 123.992 kb on + strand at 123.995 kb on + strand at 123.995 kb on + strand at 123.996 kb on - strand at 124.038 kb on + strand at 124.085 kb on + strand at 124.085 kb on + strand at 124.085 kb on + strand at 124.086 kb on - strand at 124.086 kb on - strand at 124.086 kb on - strand at 124.086 kb on - strand at 124.168 kb on - strand, within GFF134 at 124.271 kb on - strand, within GFF134 at 124.533 kb on + strand, within GFF134 at 124.656 kb on + strand, within GFF134 at 124.802 kb on + strand, within GFF134 at 124.813 kb on + strand, within GFF134 at 124.813 kb on + strand, within GFF134 at 125.040 kb on - strand at 125.040 kb on - strand at 125.093 kb on + strand at 125.098 kb on + strand at 125.099 kb on - strand at 125.178 kb on + strand at 125.198 kb on - strand at 125.262 kb on + strand at 125.930 kb on + strand at 125.931 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 123,462 - GFF133 0.58 -0.0 123,627 + GFF133 0.72 +0.0 123,730 - GFF133 0.81 -0.3 123,783 + GFF133 0.85 -1.5 123,795 + GFF133 0.86 +0.7 123,887 + +0.1 123,888 - +0.4 123,891 + +0.5 123,917 - +0.2 123,917 - -0.2 123,917 - -0.0 123,917 - +0.1 123,992 + +0.8 123,995 + -0.3 123,995 + -0.0 123,996 - +0.4 124,038 + +0.2 124,085 + +0.3 124,085 + -0.2 124,085 + +0.5 124,086 - -1.6 124,086 - -0.4 124,086 - +0.4 124,086 - -0.4 124,168 - GFF134 0.17 -0.3 124,271 - GFF134 0.27 -0.7 124,533 + GFF134 0.52 -0.1 124,656 + GFF134 0.63 -0.3 124,802 + GFF134 0.77 -0.5 124,813 + GFF134 0.78 -0.5 124,813 + GFF134 0.78 -0.2 125,040 - +0.2 125,040 - +0.2 125,093 + -0.4 125,098 + +0.1 125,099 - -0.9 125,178 + -0.5 125,198 - +0.2 125,262 + -0.6 125,930 + +0.4 125,931 - +0.0
Or see this region's nucleotide sequence