Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1318 and GFF1319 overlap by 8 nucleotides GFF1319 and GFF1320 are separated by 468 nucleotides GFF1320 and GFF1321 overlap by 2 nucleotides
GFF1318 - Lysophospholipid transporter LplT, at 25,485 to 26,687
GFF1318
GFF1319 - 2-acylglycerophosphoethanolamine acyltransferase (EC 2.3.1.40) / Acyl-[acyl-carrier-protein] synthetase (EC 6.2.1.20), at 26,680 to 28,839
GFF1319
GFF1320 - hypothetical protein, at 29,308 to 29,433
GFF1320
GFF1321 - Galactose operon repressor, GalR-LacI family of transcriptional regulators, at 29,432 to 30,460
GFF1321
Position (kb) 26
27
28
29 Strain fitness (log2 ratio) -1
0
1 at 25.763 kb on - strand, within GFF1318 at 26.078 kb on + strand, within GFF1318 at 26.217 kb on + strand, within GFF1318 at 26.326 kb on + strand, within GFF1318 at 26.369 kb on + strand, within GFF1318 at 26.403 kb on - strand, within GFF1318 at 26.503 kb on + strand, within GFF1318 at 26.670 kb on - strand at 26.673 kb on + strand at 26.822 kb on + strand at 26.823 kb on - strand at 26.830 kb on + strand at 26.862 kb on + strand at 26.862 kb on + strand at 27.044 kb on - strand, within GFF1319 at 27.044 kb on - strand, within GFF1319 at 27.062 kb on + strand, within GFF1319 at 27.153 kb on + strand, within GFF1319 at 27.255 kb on + strand, within GFF1319 at 27.309 kb on - strand, within GFF1319 at 27.472 kb on - strand, within GFF1319 at 27.472 kb on - strand, within GFF1319 at 27.477 kb on + strand, within GFF1319 at 27.486 kb on - strand, within GFF1319 at 27.486 kb on - strand, within GFF1319 at 27.536 kb on - strand, within GFF1319 at 27.678 kb on + strand, within GFF1319 at 27.679 kb on - strand, within GFF1319 at 27.866 kb on - strand, within GFF1319 at 27.891 kb on + strand, within GFF1319 at 27.892 kb on - strand, within GFF1319 at 28.134 kb on + strand, within GFF1319 at 28.222 kb on + strand, within GFF1319 at 28.293 kb on + strand, within GFF1319 at 28.293 kb on + strand, within GFF1319 at 28.350 kb on + strand, within GFF1319 at 28.350 kb on + strand, within GFF1319 at 28.350 kb on + strand, within GFF1319 at 28.350 kb on + strand, within GFF1319 at 28.579 kb on - strand, within GFF1319 at 28.641 kb on + strand at 28.643 kb on + strand at 28.665 kb on + strand at 28.668 kb on - strand at 28.668 kb on - strand at 28.668 kb on - strand at 28.668 kb on - strand at 28.743 kb on + strand at 28.744 kb on - strand at 28.747 kb on + strand at 28.890 kb on - strand at 29.095 kb on + strand at 29.096 kb on - strand at 29.167 kb on - strand at 29.201 kb on + strand at 29.202 kb on - strand at 29.228 kb on - strand at 29.228 kb on - strand at 29.228 kb on - strand at 29.228 kb on - strand at 29.422 kb on - strand at 29.425 kb on + strand at 29.425 kb on + strand at 29.425 kb on + strand at 29.425 kb on + strand at 29.425 kb on + strand at 29.426 kb on - strand at 29.426 kb on - strand at 29.429 kb on - strand at 29.443 kb on - strand at 29.452 kb on - strand at 29.498 kb on - strand at 29.723 kb on + strand, within GFF1321
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 25,763 - GFF1318 0.23 -0.2 26,078 + GFF1318 0.49 -1.0 26,217 + GFF1318 0.61 -1.3 26,326 + GFF1318 0.70 -0.6 26,369 + GFF1318 0.73 +0.2 26,403 - GFF1318 0.76 +0.2 26,503 + GFF1318 0.85 -0.4 26,670 - -0.3 26,673 + -0.6 26,822 + -1.0 26,823 - -0.3 26,830 + -0.5 26,862 + +1.3 26,862 + +1.1 27,044 - GFF1319 0.17 -0.1 27,044 - GFF1319 0.17 +0.5 27,062 + GFF1319 0.18 -0.7 27,153 + GFF1319 0.22 +0.3 27,255 + GFF1319 0.27 -0.1 27,309 - GFF1319 0.29 +0.4 27,472 - GFF1319 0.37 +0.8 27,472 - GFF1319 0.37 +0.3 27,477 + GFF1319 0.37 -1.7 27,486 - GFF1319 0.37 +0.2 27,486 - GFF1319 0.37 +1.0 27,536 - GFF1319 0.40 +0.2 27,678 + GFF1319 0.46 +0.2 27,679 - GFF1319 0.46 -0.5 27,866 - GFF1319 0.55 +0.1 27,891 + GFF1319 0.56 +0.3 27,892 - GFF1319 0.56 +0.3 28,134 + GFF1319 0.67 +0.1 28,222 + GFF1319 0.71 -0.2 28,293 + GFF1319 0.75 -0.8 28,293 + GFF1319 0.75 -0.5 28,350 + GFF1319 0.77 +0.4 28,350 + GFF1319 0.77 +0.3 28,350 + GFF1319 0.77 +0.5 28,350 + GFF1319 0.77 +0.5 28,579 - GFF1319 0.88 +0.1 28,641 + -1.0 28,643 + -0.2 28,665 + -0.0 28,668 - +0.3 28,668 - +1.1 28,668 - +0.4 28,668 - -0.4 28,743 + -1.0 28,744 - -0.6 28,747 + +0.6 28,890 - -0.0 29,095 + +0.2 29,096 - -0.2 29,167 - +0.1 29,201 + -0.2 29,202 - +0.2 29,228 - +0.9 29,228 - +0.0 29,228 - +0.8 29,228 - -0.5 29,422 - -0.3 29,425 + -0.6 29,425 + +0.1 29,425 + +0.4 29,425 + -0.3 29,425 + -0.7 29,426 - -0.5 29,426 - +0.3 29,429 - -0.4 29,443 - -0.1 29,452 - -0.6 29,498 - -0.6 29,723 + GFF1321 0.28 +0.7
Or see this region's nucleotide sequence