Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1274 and GFF1275 are separated by 235 nucleotides GFF1275 and GFF1276 are separated by 286 nucleotides GFF1276 and GFF1277 overlap by 29 nucleotides
GFF1274 - Phage outer membrane lytic protein Rz; Endopeptidase (EC 3.4.-.-), at 1,782 to 2,255
GFF1274
GFF1275 - FIG01045439: hypothetical protein, at 2,491 to 2,892
GFF1275
GFF1276 - Terminase small subunit, at 3,179 to 3,724
GFF1276
GFF1277 - Phage terminase, large subunit, at 3,696 to 5,627
GFF1277
Position (kb)
3
4 Strain fitness (log2 ratio)
-1
0
1
2 at 2.221 kb on - strand at 2.284 kb on + strand at 2.339 kb on + strand at 2.369 kb on + strand at 2.547 kb on + strand, within GFF1275 at 2.548 kb on - strand, within GFF1275 at 2.561 kb on - strand, within GFF1275 at 2.574 kb on - strand, within GFF1275 at 2.574 kb on - strand, within GFF1275 at 2.673 kb on - strand, within GFF1275 at 2.673 kb on - strand, within GFF1275 at 2.736 kb on + strand, within GFF1275 at 2.737 kb on - strand, within GFF1275 at 2.738 kb on + strand, within GFF1275 at 2.739 kb on - strand, within GFF1275 at 2.739 kb on - strand, within GFF1275 at 2.743 kb on - strand, within GFF1275 at 2.896 kb on + strand at 3.476 kb on - strand, within GFF1276 at 3.524 kb on + strand, within GFF1276 at 3.525 kb on - strand, within GFF1276 at 3.553 kb on - strand, within GFF1276 at 3.585 kb on + strand, within GFF1276 at 3.586 kb on - strand, within GFF1276 at 3.597 kb on - strand, within GFF1276 at 3.744 kb on - strand at 3.807 kb on + strand at 3.807 kb on + strand at 3.807 kb on + strand at 3.808 kb on - strand at 3.811 kb on - strand at 3.811 kb on - strand at 3.910 kb on + strand, within GFF1277 at 3.921 kb on - strand, within GFF1277 at 3.921 kb on - strand, within GFF1277 at 3.970 kb on - strand, within GFF1277 at 4.078 kb on + strand, within GFF1277 at 4.295 kb on + strand, within GFF1277 at 4.295 kb on + strand, within GFF1277 at 4.438 kb on - strand, within GFF1277 at 4.460 kb on + strand, within GFF1277 at 4.506 kb on + strand, within GFF1277 at 4.507 kb on - strand, within GFF1277
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 2,221 - +0.6 2,284 + -0.4 2,339 + -0.2 2,369 + +0.2 2,547 + GFF1275 0.14 -1.4 2,548 - GFF1275 0.14 -0.3 2,561 - GFF1275 0.17 +0.1 2,574 - GFF1275 0.21 +0.6 2,574 - GFF1275 0.21 +0.5 2,673 - GFF1275 0.45 +0.1 2,673 - GFF1275 0.45 -0.1 2,736 + GFF1275 0.61 +0.4 2,737 - GFF1275 0.61 +0.1 2,738 + GFF1275 0.61 -0.8 2,739 - GFF1275 0.62 +0.5 2,739 - GFF1275 0.62 -0.0 2,743 - GFF1275 0.63 -0.0 2,896 + -0.1 3,476 - GFF1276 0.54 +0.2 3,524 + GFF1276 0.63 +0.6 3,525 - GFF1276 0.63 +0.0 3,553 - GFF1276 0.68 +0.1 3,585 + GFF1276 0.74 +0.3 3,586 - GFF1276 0.75 +0.1 3,597 - GFF1276 0.77 +0.9 3,744 - -0.3 3,807 + -0.6 3,807 + +0.9 3,807 + -0.0 3,808 - -0.3 3,811 - +0.5 3,811 - +0.9 3,910 + GFF1277 0.11 -0.1 3,921 - GFF1277 0.12 -0.3 3,921 - GFF1277 0.12 +0.4 3,970 - GFF1277 0.14 +0.5 4,078 + GFF1277 0.20 +2.2 4,295 + GFF1277 0.31 +0.3 4,295 + GFF1277 0.31 +0.7 4,438 - GFF1277 0.38 -0.3 4,460 + GFF1277 0.40 +0.8 4,506 + GFF1277 0.42 +0.1 4,507 - GFF1277 0.42 -0.4
Or see this region's nucleotide sequence