Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1253 and GFF1254 are separated by 56 nucleotides GFF1254 and GFF1255 are separated by 31 nucleotides GFF1255 and GFF1256 are separated by 498 nucleotides
GFF1253 - FIG01047572: hypothetical protein, at 81,688 to 82,023
GFF1253
GFF1254 - FIG01046430: hypothetical protein, at 82,080 to 82,646
GFF1254
GFF1255 - Transposase, at 82,678 to 83,535
GFF1255
GFF1256 - Chromosome (plasmid) partitioning protein ParA, at 84,034 to 85,239
GFF1256
Position (kb)
82
83
84 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 81.697 kb on - strand at 81.759 kb on + strand, within GFF1253 at 81.794 kb on + strand, within GFF1253 at 81.838 kb on - strand, within GFF1253 at 81.843 kb on + strand, within GFF1253 at 81.859 kb on + strand, within GFF1253 at 81.886 kb on - strand, within GFF1253 at 81.941 kb on - strand, within GFF1253 at 81.941 kb on - strand, within GFF1253 at 81.941 kb on - strand, within GFF1253 at 82.012 kb on - strand at 82.012 kb on - strand at 82.035 kb on + strand at 82.049 kb on + strand at 82.049 kb on + strand at 82.050 kb on - strand at 82.317 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.378 kb on - strand, within GFF1254 at 82.378 kb on - strand, within GFF1254 at 82.414 kb on - strand, within GFF1254 at 82.485 kb on + strand, within GFF1254 at 82.517 kb on + strand, within GFF1254 at 82.537 kb on + strand, within GFF1254 at 82.697 kb on - strand at 82.888 kb on - strand, within GFF1255 at 82.888 kb on - strand, within GFF1255 at 82.931 kb on - strand, within GFF1255 at 82.931 kb on - strand, within GFF1255 at 82.970 kb on - strand, within GFF1255 at 82.970 kb on - strand, within GFF1255 at 82.970 kb on - strand, within GFF1255 at 82.970 kb on - strand, within GFF1255 at 82.970 kb on - strand, within GFF1255 at 82.970 kb on - strand, within GFF1255 at 82.970 kb on - strand, within GFF1255 at 82.972 kb on - strand, within GFF1255 at 82.972 kb on - strand, within GFF1255 at 82.972 kb on - strand, within GFF1255 at 82.972 kb on - strand, within GFF1255 at 82.972 kb on - strand, within GFF1255 at 82.973 kb on + strand, within GFF1255 at 82.973 kb on + strand, within GFF1255 at 82.973 kb on + strand, within GFF1255 at 82.973 kb on + strand, within GFF1255 at 82.974 kb on - strand, within GFF1255 at 82.974 kb on - strand, within GFF1255 at 82.987 kb on + strand, within GFF1255 at 82.991 kb on - strand, within GFF1255 at 83.071 kb on - strand, within GFF1255 at 83.169 kb on - strand, within GFF1255 at 83.169 kb on - strand, within GFF1255 at 83.249 kb on + strand, within GFF1255 at 83.250 kb on - strand, within GFF1255 at 83.250 kb on - strand, within GFF1255 at 83.324 kb on + strand, within GFF1255 at 83.346 kb on + strand, within GFF1255 at 83.347 kb on - strand, within GFF1255 at 83.388 kb on + strand, within GFF1255 at 83.389 kb on - strand, within GFF1255 at 83.389 kb on - strand, within GFF1255 at 83.421 kb on - strand, within GFF1255 at 83.438 kb on + strand, within GFF1255 at 83.536 kb on - strand at 83.654 kb on - strand at 83.755 kb on + strand at 83.791 kb on - strand at 83.797 kb on + strand at 83.797 kb on + strand at 83.797 kb on + strand at 83.910 kb on + strand at 83.911 kb on - strand at 83.930 kb on - strand at 84.135 kb on + strand at 84.172 kb on + strand, within GFF1256 at 84.173 kb on - strand, within GFF1256 at 84.173 kb on - strand, within GFF1256 at 84.267 kb on + strand, within GFF1256 at 84.365 kb on + strand, within GFF1256 at 84.371 kb on + strand, within GFF1256 at 84.376 kb on - strand, within GFF1256
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 81,697 - -0.1 81,759 + GFF1253 0.21 -0.1 81,794 + GFF1253 0.32 +1.0 81,838 - GFF1253 0.45 +0.4 81,843 + GFF1253 0.46 -0.8 81,859 + GFF1253 0.51 +0.3 81,886 - GFF1253 0.59 -0.4 81,941 - GFF1253 0.75 -0.2 81,941 - GFF1253 0.75 +0.9 81,941 - GFF1253 0.75 +0.1 82,012 - -0.0 82,012 - -0.2 82,035 + +0.1 82,049 + -0.1 82,049 + -1.0 82,050 - -0.3 82,317 + GFF1254 0.42 +0.2 82,377 + GFF1254 0.52 +0.4 82,377 + GFF1254 0.52 -0.2 82,377 + GFF1254 0.52 +0.3 82,377 + GFF1254 0.52 +0.2 82,377 + GFF1254 0.52 +0.2 82,378 - GFF1254 0.53 -0.2 82,378 - GFF1254 0.53 +0.0 82,414 - GFF1254 0.59 -0.0 82,485 + GFF1254 0.71 -0.3 82,517 + GFF1254 0.77 +0.0 82,537 + GFF1254 0.81 +0.2 82,697 - -0.2 82,888 - GFF1255 0.24 -1.4 82,888 - GFF1255 0.24 -0.3 82,931 - GFF1255 0.29 +0.2 82,931 - GFF1255 0.29 -0.4 82,970 - GFF1255 0.34 -1.1 82,970 - GFF1255 0.34 -0.6 82,970 - GFF1255 0.34 -0.4 82,970 - GFF1255 0.34 +1.1 82,970 - GFF1255 0.34 +0.1 82,970 - GFF1255 0.34 -0.0 82,970 - GFF1255 0.34 +0.2 82,972 - GFF1255 0.34 +0.1 82,972 - GFF1255 0.34 +0.1 82,972 - GFF1255 0.34 +0.2 82,972 - GFF1255 0.34 +0.0 82,972 - GFF1255 0.34 -0.2 82,973 + GFF1255 0.34 -0.1 82,973 + GFF1255 0.34 +0.6 82,973 + GFF1255 0.34 -0.0 82,973 + GFF1255 0.34 +0.5 82,974 - GFF1255 0.34 -0.1 82,974 - GFF1255 0.34 -0.8 82,987 + GFF1255 0.36 +0.5 82,991 - GFF1255 0.36 -0.2 83,071 - GFF1255 0.46 +0.4 83,169 - GFF1255 0.57 -0.4 83,169 - GFF1255 0.57 +0.5 83,249 + GFF1255 0.67 +0.5 83,250 - GFF1255 0.67 +0.3 83,250 - GFF1255 0.67 -0.8 83,324 + GFF1255 0.75 +0.1 83,346 + GFF1255 0.78 +0.2 83,347 - GFF1255 0.78 +0.5 83,388 + GFF1255 0.83 +0.4 83,389 - GFF1255 0.83 +1.0 83,389 - GFF1255 0.83 -0.0 83,421 - GFF1255 0.87 -0.1 83,438 + GFF1255 0.89 +0.7 83,536 - +0.1 83,654 - -0.2 83,755 + -1.3 83,791 - -1.2 83,797 + +0.6 83,797 + +0.2 83,797 + -0.2 83,910 + -0.0 83,911 - -0.5 83,930 - +0.6 84,135 + -2.8 84,172 + GFF1256 0.11 -1.8 84,173 - GFF1256 0.12 +0.2 84,173 - GFF1256 0.12 -1.9 84,267 + GFF1256 0.19 -2.8 84,365 + GFF1256 0.27 -2.8 84,371 + GFF1256 0.28 +0.2 84,376 - GFF1256 0.28 -0.8
Or see this region's nucleotide sequence