Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1201 and GFF1202 are separated by 50 nucleotides GFF1202 and GFF1203 overlap by 11 nucleotides GFF1203 and GFF1204 are separated by 43 nucleotides GFF1204 and GFF1205 overlap by 35 nucleotides GFF1205 and GFF1206 overlap by 22 nucleotides GFF1206 and GFF1207 overlap by 16 nucleotides GFF1207 and GFF1208 are separated by 361 nucleotides GFF1208 and GFF1209 are separated by 10 nucleotides
GFF1201 - Outer membrane protein assembly factor YaeT precursor, at 45,862 to 46,707
GFF1201
GFF1202 - hypothetical protein, at 46,758 to 46,904
GFF1202
GFF1203 - Thiol:disulfide interchange protein dsbA precursor, at 46,894 to 47,547
GFF1203
GFF1204 - hypothetical protein, at 47,591 to 47,704
GFF1204
GFF1205 - hypothetical protein, at 47,670 to 47,813
GFF1205
GFF1206 - Putative GerE-family regulatory protein, at 47,792 to 48,142
GFF1206
GFF1207 - Probable regulatory protein, at 48,127 to 48,285
GFF1207
GFF1208 - hypothetical protein, at 48,647 to 48,760
GFF1208
GFF1209 - putative outer membrane protein, at 48,771 to 49,658
GFF1209
Position (kb) 47
48 Strain fitness (log2 ratio) -1
0
1 at 46.689 kb on + strand at 46.693 kb on + strand at 46.693 kb on + strand at 46.694 kb on - strand at 46.694 kb on - strand at 46.694 kb on - strand at 46.775 kb on + strand, within GFF1202 at 46.878 kb on + strand, within GFF1202 at 47.001 kb on - strand, within GFF1203 at 47.015 kb on + strand, within GFF1203 at 47.016 kb on - strand, within GFF1203 at 47.016 kb on - strand, within GFF1203 at 47.043 kb on - strand, within GFF1203 at 47.043 kb on - strand, within GFF1203 at 47.065 kb on + strand, within GFF1203 at 47.065 kb on + strand, within GFF1203 at 47.099 kb on - strand, within GFF1203 at 47.099 kb on - strand, within GFF1203 at 47.099 kb on - strand, within GFF1203 at 47.100 kb on + strand, within GFF1203 at 47.101 kb on - strand, within GFF1203 at 47.142 kb on + strand, within GFF1203 at 47.300 kb on + strand, within GFF1203 at 47.301 kb on - strand, within GFF1203 at 47.301 kb on - strand, within GFF1203 at 47.330 kb on - strand, within GFF1203 at 47.574 kb on - strand at 47.574 kb on - strand at 47.726 kb on + strand, within GFF1205 at 47.860 kb on - strand, within GFF1206 at 48.001 kb on + strand, within GFF1206 at 48.065 kb on - strand, within GFF1206 at 48.088 kb on - strand, within GFF1206 at 48.092 kb on + strand, within GFF1206 at 48.142 kb on + strand at 48.210 kb on - strand, within GFF1207 at 48.229 kb on - strand, within GFF1207 at 48.229 kb on - strand, within GFF1207 at 48.229 kb on - strand, within GFF1207 at 48.439 kb on - strand at 48.521 kb on + strand at 48.587 kb on - strand at 48.598 kb on - strand at 48.629 kb on + strand at 48.645 kb on + strand at 48.666 kb on + strand, within GFF1208 at 48.689 kb on + strand, within GFF1208 at 48.731 kb on + strand, within GFF1208 at 48.765 kb on + strand at 48.790 kb on - strand at 48.812 kb on + strand at 48.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 46,689 + +0.3 46,693 + +0.2 46,693 + +0.2 46,694 - +0.1 46,694 - +0.1 46,694 - -0.9 46,775 + GFF1202 0.12 +0.8 46,878 + GFF1202 0.82 +0.0 47,001 - GFF1203 0.16 +0.4 47,015 + GFF1203 0.19 +0.2 47,016 - GFF1203 0.19 -0.9 47,016 - GFF1203 0.19 -0.0 47,043 - GFF1203 0.23 +0.3 47,043 - GFF1203 0.23 +0.1 47,065 + GFF1203 0.26 +0.0 47,065 + GFF1203 0.26 +0.1 47,099 - GFF1203 0.31 +0.4 47,099 - GFF1203 0.31 +0.8 47,099 - GFF1203 0.31 +0.0 47,100 + GFF1203 0.31 +1.0 47,101 - GFF1203 0.32 +0.2 47,142 + GFF1203 0.38 -0.0 47,300 + GFF1203 0.62 -0.0 47,301 - GFF1203 0.62 -0.2 47,301 - GFF1203 0.62 -0.2 47,330 - GFF1203 0.67 -0.7 47,574 - -0.2 47,574 - -0.4 47,726 + GFF1205 0.39 -1.3 47,860 - GFF1206 0.19 +0.2 48,001 + GFF1206 0.60 -1.1 48,065 - GFF1206 0.78 +0.6 48,088 - GFF1206 0.84 +0.1 48,092 + GFF1206 0.85 -1.1 48,142 + -1.2 48,210 - GFF1207 0.52 +0.0 48,229 - GFF1207 0.64 +0.6 48,229 - GFF1207 0.64 +0.3 48,229 - GFF1207 0.64 -0.1 48,439 - +0.4 48,521 + +0.5 48,587 - +0.7 48,598 - +0.2 48,629 + -0.2 48,645 + +0.1 48,666 + GFF1208 0.17 -0.3 48,689 + GFF1208 0.37 -0.4 48,731 + GFF1208 0.74 +0.7 48,765 + +0.5 48,790 - -0.5 48,812 + +0.0 48,813 - +0.6
Or see this region's nucleotide sequence