Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1182 and GFF1183 are separated by 21 nucleotides GFF1183 and GFF1184 are separated by 35 nucleotides
GFF1182 - IncF plasmid conjugative transfer protein TraG, at 26,131 to 28,953
GFF1182
GFF1183 - IncF plasmid conjugative transfer surface exclusion protein TraS, at 28,975 to 29,463
GFF1183
GFF1184 - IncF plasmid conjugative transfer surface exclusion protein TraT, at 29,499 to 30,230
GFF1184
Position (kb) 28
29
30 Strain fitness (log2 ratio) -1
0
1 at 28.000 kb on + strand, within GFF1182 at 28.001 kb on - strand, within GFF1182 at 28.001 kb on - strand, within GFF1182 at 28.028 kb on + strand, within GFF1182 at 28.205 kb on - strand, within GFF1182 at 28.205 kb on - strand, within GFF1182 at 28.252 kb on - strand, within GFF1182 at 28.309 kb on + strand, within GFF1182 at 28.309 kb on + strand, within GFF1182 at 28.310 kb on - strand, within GFF1182 at 28.318 kb on + strand, within GFF1182 at 28.348 kb on + strand, within GFF1182 at 28.535 kb on + strand, within GFF1182 at 28.590 kb on + strand, within GFF1182 at 28.591 kb on - strand, within GFF1182 at 28.617 kb on + strand, within GFF1182 at 28.683 kb on + strand at 28.684 kb on - strand at 28.684 kb on - strand at 28.687 kb on - strand at 28.764 kb on + strand at 28.820 kb on - strand at 28.852 kb on + strand at 29.273 kb on + strand, within GFF1183 at 29.279 kb on + strand, within GFF1183 at 29.315 kb on - strand, within GFF1183 at 29.371 kb on + strand, within GFF1183 at 29.372 kb on - strand, within GFF1183 at 29.372 kb on - strand, within GFF1183 at 29.395 kb on - strand, within GFF1183 at 29.493 kb on - strand at 29.658 kb on + strand, within GFF1184 at 29.658 kb on + strand, within GFF1184 at 29.676 kb on - strand, within GFF1184 at 29.739 kb on + strand, within GFF1184 at 29.742 kb on - strand, within GFF1184 at 29.769 kb on + strand, within GFF1184 at 29.769 kb on + strand, within GFF1184 at 29.769 kb on + strand, within GFF1184 at 29.773 kb on - strand, within GFF1184 at 29.895 kb on + strand, within GFF1184 at 29.895 kb on + strand, within GFF1184 at 29.896 kb on - strand, within GFF1184 at 29.982 kb on - strand, within GFF1184 at 29.985 kb on - strand, within GFF1184 at 30.119 kb on + strand, within GFF1184 at 30.120 kb on - strand, within GFF1184 at 30.120 kb on - strand, within GFF1184 at 30.123 kb on + strand, within GFF1184
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 28,000 + GFF1182 0.66 +0.3 28,001 - GFF1182 0.66 -0.5 28,001 - GFF1182 0.66 -0.5 28,028 + GFF1182 0.67 -0.1 28,205 - GFF1182 0.73 +0.4 28,205 - GFF1182 0.73 -0.1 28,252 - GFF1182 0.75 -0.1 28,309 + GFF1182 0.77 +0.5 28,309 + GFF1182 0.77 +0.3 28,310 - GFF1182 0.77 -0.1 28,318 + GFF1182 0.77 -0.2 28,348 + GFF1182 0.79 -0.2 28,535 + GFF1182 0.85 -0.3 28,590 + GFF1182 0.87 +0.2 28,591 - GFF1182 0.87 -0.1 28,617 + GFF1182 0.88 -0.1 28,683 + -0.2 28,684 - +1.4 28,684 - -0.4 28,687 - -0.4 28,764 + -0.7 28,820 - +0.1 28,852 + +0.5 29,273 + GFF1183 0.61 +0.8 29,279 + GFF1183 0.62 -0.4 29,315 - GFF1183 0.70 -0.1 29,371 + GFF1183 0.81 +0.6 29,372 - GFF1183 0.81 -0.3 29,372 - GFF1183 0.81 +0.2 29,395 - GFF1183 0.86 -0.4 29,493 - +0.5 29,658 + GFF1184 0.22 +0.2 29,658 + GFF1184 0.22 +0.8 29,676 - GFF1184 0.24 -0.2 29,739 + GFF1184 0.33 -1.5 29,742 - GFF1184 0.33 +0.4 29,769 + GFF1184 0.37 -0.6 29,769 + GFF1184 0.37 +0.2 29,769 + GFF1184 0.37 -0.2 29,773 - GFF1184 0.37 -0.5 29,895 + GFF1184 0.54 +1.1 29,895 + GFF1184 0.54 +0.3 29,896 - GFF1184 0.54 -0.4 29,982 - GFF1184 0.66 +0.5 29,985 - GFF1184 0.66 -0.2 30,119 + GFF1184 0.85 +0.2 30,120 - GFF1184 0.85 -0.1 30,120 - GFF1184 0.85 +0.2 30,123 + GFF1184 0.85 -0.0
Or see this region's nucleotide sequence