Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1181 and GFF1182 overlap by 4 nucleotides GFF1182 and GFF1183 are separated by 21 nucleotides GFF1183 and GFF1184 are separated by 35 nucleotides
GFF1181 - IncF plasmid conjugative transfer pilus assembly protein TraH, at 24,752 to 26,134
GFF1181
GFF1182 - IncF plasmid conjugative transfer protein TraG, at 26,131 to 28,953
GFF1182
GFF1183 - IncF plasmid conjugative transfer surface exclusion protein TraS, at 28,975 to 29,463
GFF1183
GFF1184 - IncF plasmid conjugative transfer surface exclusion protein TraT, at 29,499 to 30,230
GFF1184
Position (kb)
26
27
28
29 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 25.189 kb on + strand, within GFF1181 at 25.195 kb on + strand, within GFF1181 at 25.195 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.340 kb on - strand, within GFF1181 at 25.565 kb on - strand, within GFF1181 at 25.600 kb on - strand, within GFF1181 at 25.600 kb on - strand, within GFF1181 at 25.601 kb on + strand, within GFF1181 at 25.601 kb on + strand, within GFF1181 at 25.602 kb on - strand, within GFF1181 at 25.602 kb on - strand, within GFF1181 at 25.621 kb on + strand, within GFF1181 at 25.747 kb on + strand, within GFF1181 at 25.762 kb on + strand, within GFF1181 at 25.763 kb on - strand, within GFF1181 at 25.839 kb on - strand, within GFF1181 at 25.853 kb on + strand, within GFF1181 at 25.853 kb on + strand, within GFF1181 at 25.854 kb on - strand, within GFF1181 at 25.880 kb on + strand, within GFF1181 at 25.881 kb on - strand, within GFF1181 at 25.881 kb on - strand, within GFF1181 at 25.931 kb on + strand, within GFF1181 at 25.931 kb on + strand, within GFF1181 at 25.932 kb on - strand, within GFF1181 at 25.932 kb on - strand, within GFF1181 at 25.954 kb on + strand, within GFF1181 at 25.954 kb on + strand, within GFF1181 at 25.955 kb on - strand, within GFF1181 at 26.058 kb on - strand at 26.058 kb on - strand at 26.143 kb on + strand at 26.144 kb on - strand at 26.144 kb on - strand at 26.173 kb on - strand at 26.173 kb on - strand at 26.268 kb on + strand at 26.269 kb on - strand at 26.286 kb on + strand at 26.287 kb on - strand at 26.287 kb on - strand at 26.331 kb on - strand at 26.360 kb on + strand at 26.360 kb on + strand at 26.361 kb on - strand at 26.367 kb on - strand at 26.367 kb on - strand at 26.406 kb on + strand at 26.476 kb on + strand, within GFF1182 at 26.477 kb on - strand, within GFF1182 at 26.477 kb on - strand, within GFF1182 at 26.477 kb on - strand, within GFF1182 at 26.512 kb on + strand, within GFF1182 at 26.513 kb on - strand, within GFF1182 at 26.526 kb on + strand, within GFF1182 at 26.527 kb on - strand, within GFF1182 at 26.609 kb on - strand, within GFF1182 at 26.634 kb on + strand, within GFF1182 at 26.635 kb on - strand, within GFF1182 at 26.635 kb on - strand, within GFF1182 at 26.635 kb on - strand, within GFF1182 at 26.654 kb on - strand, within GFF1182 at 26.692 kb on - strand, within GFF1182 at 26.731 kb on + strand, within GFF1182 at 26.731 kb on + strand, within GFF1182 at 26.803 kb on - strand, within GFF1182 at 26.830 kb on + strand, within GFF1182 at 26.831 kb on - strand, within GFF1182 at 26.909 kb on - strand, within GFF1182 at 26.950 kb on - strand, within GFF1182 at 26.950 kb on - strand, within GFF1182 at 26.991 kb on + strand, within GFF1182 at 27.120 kb on + strand, within GFF1182 at 27.120 kb on + strand, within GFF1182 at 27.208 kb on + strand, within GFF1182 at 27.209 kb on - strand, within GFF1182 at 27.212 kb on + strand, within GFF1182 at 27.212 kb on + strand, within GFF1182 at 27.212 kb on + strand, within GFF1182 at 27.213 kb on - strand, within GFF1182 at 27.213 kb on - strand, within GFF1182 at 27.213 kb on - strand, within GFF1182 at 27.213 kb on - strand, within GFF1182 at 27.250 kb on + strand, within GFF1182 at 27.251 kb on - strand, within GFF1182 at 27.329 kb on + strand, within GFF1182 at 27.407 kb on + strand, within GFF1182 at 27.407 kb on + strand, within GFF1182 at 27.509 kb on - strand, within GFF1182 at 27.558 kb on + strand, within GFF1182 at 27.567 kb on + strand, within GFF1182 at 27.568 kb on - strand, within GFF1182 at 27.568 kb on - strand, within GFF1182 at 27.639 kb on + strand, within GFF1182 at 27.639 kb on + strand, within GFF1182 at 27.639 kb on + strand, within GFF1182 at 27.640 kb on - strand, within GFF1182 at 27.640 kb on - strand, within GFF1182 at 27.780 kb on + strand, within GFF1182 at 27.798 kb on + strand, within GFF1182 at 27.933 kb on + strand, within GFF1182 at 27.933 kb on + strand, within GFF1182 at 27.933 kb on + strand, within GFF1182 at 27.934 kb on - strand, within GFF1182 at 27.934 kb on - strand, within GFF1182 at 27.937 kb on - strand, within GFF1182 at 27.937 kb on - strand, within GFF1182 at 27.937 kb on - strand, within GFF1182 at 28.000 kb on + strand, within GFF1182 at 28.001 kb on - strand, within GFF1182 at 28.001 kb on - strand, within GFF1182 at 28.028 kb on + strand, within GFF1182 at 28.205 kb on - strand, within GFF1182 at 28.205 kb on - strand, within GFF1182 at 28.252 kb on - strand, within GFF1182 at 28.309 kb on + strand, within GFF1182 at 28.309 kb on + strand, within GFF1182 at 28.310 kb on - strand, within GFF1182 at 28.318 kb on + strand, within GFF1182 at 28.348 kb on + strand, within GFF1182 at 28.535 kb on + strand, within GFF1182 at 28.590 kb on + strand, within GFF1182 at 28.591 kb on - strand, within GFF1182 at 28.617 kb on + strand, within GFF1182 at 28.683 kb on + strand at 28.684 kb on - strand at 28.684 kb on - strand at 28.687 kb on - strand at 28.764 kb on + strand at 28.820 kb on - strand at 28.852 kb on + strand at 29.273 kb on + strand, within GFF1183 at 29.279 kb on + strand, within GFF1183 at 29.315 kb on - strand, within GFF1183 at 29.371 kb on + strand, within GFF1183 at 29.372 kb on - strand, within GFF1183 at 29.372 kb on - strand, within GFF1183 at 29.395 kb on - strand, within GFF1183 at 29.493 kb on - strand at 29.658 kb on + strand, within GFF1184 at 29.658 kb on + strand, within GFF1184 at 29.676 kb on - strand, within GFF1184 at 29.739 kb on + strand, within GFF1184 at 29.742 kb on - strand, within GFF1184 at 29.769 kb on + strand, within GFF1184 at 29.769 kb on + strand, within GFF1184 at 29.769 kb on + strand, within GFF1184 at 29.773 kb on - strand, within GFF1184 at 29.895 kb on + strand, within GFF1184 at 29.895 kb on + strand, within GFF1184 at 29.896 kb on - strand, within GFF1184
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 25,189 + GFF1181 0.32 +0.4 25,195 + GFF1181 0.32 +0.1 25,195 + GFF1181 0.32 -1.2 25,339 + GFF1181 0.42 -0.0 25,339 + GFF1181 0.42 -1.6 25,339 + GFF1181 0.42 -0.9 25,339 + GFF1181 0.42 -0.2 25,340 - GFF1181 0.43 +0.2 25,565 - GFF1181 0.59 +0.2 25,600 - GFF1181 0.61 +0.1 25,600 - GFF1181 0.61 -0.2 25,601 + GFF1181 0.61 -0.2 25,601 + GFF1181 0.61 +0.0 25,602 - GFF1181 0.61 +0.2 25,602 - GFF1181 0.61 -0.8 25,621 + GFF1181 0.63 +0.0 25,747 + GFF1181 0.72 -1.6 25,762 + GFF1181 0.73 +0.3 25,763 - GFF1181 0.73 +2.0 25,839 - GFF1181 0.79 +0.0 25,853 + GFF1181 0.80 +0.0 25,853 + GFF1181 0.80 +0.5 25,854 - GFF1181 0.80 -0.6 25,880 + GFF1181 0.82 -0.1 25,881 - GFF1181 0.82 +0.8 25,881 - GFF1181 0.82 +0.7 25,931 + GFF1181 0.85 +0.0 25,931 + GFF1181 0.85 -0.3 25,932 - GFF1181 0.85 +0.4 25,932 - GFF1181 0.85 -0.2 25,954 + GFF1181 0.87 -0.1 25,954 + GFF1181 0.87 +0.5 25,955 - GFF1181 0.87 -0.0 26,058 - +0.5 26,058 - -0.7 26,143 + +0.1 26,144 - +0.8 26,144 - +0.4 26,173 - -0.5 26,173 - -0.7 26,268 + +0.2 26,269 - -0.7 26,286 + +0.2 26,287 - +0.2 26,287 - -0.1 26,331 - -1.4 26,360 + +0.2 26,360 + +0.2 26,361 - +0.4 26,367 - -0.5 26,367 - +0.1 26,406 + -0.2 26,476 + GFF1182 0.12 +0.1 26,477 - GFF1182 0.12 +1.0 26,477 - GFF1182 0.12 -0.3 26,477 - GFF1182 0.12 +0.7 26,512 + GFF1182 0.13 +0.0 26,513 - GFF1182 0.14 -0.3 26,526 + GFF1182 0.14 -1.0 26,527 - GFF1182 0.14 +0.2 26,609 - GFF1182 0.17 +0.2 26,634 + GFF1182 0.18 -0.3 26,635 - GFF1182 0.18 -0.3 26,635 - GFF1182 0.18 -0.4 26,635 - GFF1182 0.18 -0.9 26,654 - GFF1182 0.19 +0.4 26,692 - GFF1182 0.20 -0.1 26,731 + GFF1182 0.21 -0.8 26,731 + GFF1182 0.21 -0.1 26,803 - GFF1182 0.24 -2.1 26,830 + GFF1182 0.25 +0.1 26,831 - GFF1182 0.25 -0.1 26,909 - GFF1182 0.28 -0.6 26,950 - GFF1182 0.29 +0.2 26,950 - GFF1182 0.29 +0.2 26,991 + GFF1182 0.30 +0.1 27,120 + GFF1182 0.35 +0.2 27,120 + GFF1182 0.35 -0.2 27,208 + GFF1182 0.38 +0.4 27,209 - GFF1182 0.38 -0.0 27,212 + GFF1182 0.38 +0.3 27,212 + GFF1182 0.38 -0.3 27,212 + GFF1182 0.38 +0.4 27,213 - GFF1182 0.38 -0.7 27,213 - GFF1182 0.38 -0.1 27,213 - GFF1182 0.38 +0.4 27,213 - GFF1182 0.38 -0.5 27,250 + GFF1182 0.40 -0.0 27,251 - GFF1182 0.40 +1.2 27,329 + GFF1182 0.42 -0.1 27,407 + GFF1182 0.45 -0.5 27,407 + GFF1182 0.45 -1.5 27,509 - GFF1182 0.49 -0.1 27,558 + GFF1182 0.51 -0.1 27,567 + GFF1182 0.51 -0.2 27,568 - GFF1182 0.51 -0.7 27,568 - GFF1182 0.51 -1.1 27,639 + GFF1182 0.53 -1.3 27,639 + GFF1182 0.53 +0.5 27,639 + GFF1182 0.53 -0.1 27,640 - GFF1182 0.53 +0.4 27,640 - GFF1182 0.53 +0.3 27,780 + GFF1182 0.58 -0.4 27,798 + GFF1182 0.59 +0.2 27,933 + GFF1182 0.64 -0.0 27,933 + GFF1182 0.64 +2.1 27,933 + GFF1182 0.64 -0.3 27,934 - GFF1182 0.64 +0.0 27,934 - GFF1182 0.64 -0.4 27,937 - GFF1182 0.64 -0.2 27,937 - GFF1182 0.64 -0.6 27,937 - GFF1182 0.64 -0.1 28,000 + GFF1182 0.66 +0.3 28,001 - GFF1182 0.66 -0.5 28,001 - GFF1182 0.66 -0.5 28,028 + GFF1182 0.67 -0.1 28,205 - GFF1182 0.73 +0.4 28,205 - GFF1182 0.73 -0.1 28,252 - GFF1182 0.75 -0.1 28,309 + GFF1182 0.77 +0.5 28,309 + GFF1182 0.77 +0.3 28,310 - GFF1182 0.77 -0.1 28,318 + GFF1182 0.77 -0.2 28,348 + GFF1182 0.79 -0.2 28,535 + GFF1182 0.85 -0.3 28,590 + GFF1182 0.87 +0.2 28,591 - GFF1182 0.87 -0.1 28,617 + GFF1182 0.88 -0.1 28,683 + -0.2 28,684 - +1.4 28,684 - -0.4 28,687 - -0.4 28,764 + -0.7 28,820 - +0.1 28,852 + +0.5 29,273 + GFF1183 0.61 +0.8 29,279 + GFF1183 0.62 -0.4 29,315 - GFF1183 0.70 -0.1 29,371 + GFF1183 0.81 +0.6 29,372 - GFF1183 0.81 -0.3 29,372 - GFF1183 0.81 +0.2 29,395 - GFF1183 0.86 -0.4 29,493 - +0.5 29,658 + GFF1184 0.22 +0.2 29,658 + GFF1184 0.22 +0.8 29,676 - GFF1184 0.24 -0.2 29,739 + GFF1184 0.33 -1.5 29,742 - GFF1184 0.33 +0.4 29,769 + GFF1184 0.37 -0.6 29,769 + GFF1184 0.37 +0.2 29,769 + GFF1184 0.37 -0.2 29,773 - GFF1184 0.37 -0.5 29,895 + GFF1184 0.54 +1.1 29,895 + GFF1184 0.54 +0.3 29,896 - GFF1184 0.54 -0.4
Or see this region's nucleotide sequence