Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1159 and GFF1160 overlap by 11 nucleotides GFF1160 and GFF1161 overlap by 14 nucleotides GFF1161 and GFF1162 overlap by 4 nucleotides GFF1162 and GFF1163 are separated by 134 nucleotides GFF1163 and GFF1164 overlap by 8 nucleotides GFF1164 and GFF1165 overlap by 4 nucleotides GFF1165 and GFF1166 are separated by 0 nucleotides
GFF1159 - IncF plasmid conjugative transfer pilus assembly protein TraB, at 10,923 to 12,344
GFF1159
GFF1160 - IncF plasmid conjugative transfer protein TraP, at 12,334 to 12,930
GFF1160
GFF1161 - IncF plasmid conjugative transfer protein TrbD, at 12,917 to 13,153
GFF1161
GFF1162 - IncF plasmid conjugative transfer pilus assembly protein TraV, at 13,150 to 13,665
GFF1162
GFF1163 - IncF plasmid conjugative transfer protein TraR, at 13,800 to 14,021
GFF1163
GFF1164 - Conjugative transfer protein PSLT087, at 14,014 to 14,481
GFF1164
GFF1165 - hypothetical protein, at 14,478 to 14,597
GFF1165
GFF1166 - FIG01045198: hypothetical protein, at 14,598 to 14,861
GFF1166
Position (kb) 13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 12.220 kb on + strand at 12.220 kb on + strand at 12.242 kb on + strand at 12.243 kb on - strand at 12.305 kb on + strand at 12.332 kb on - strand at 12.342 kb on + strand at 12.384 kb on + strand at 12.385 kb on - strand at 12.385 kb on - strand at 12.424 kb on + strand, within GFF1160 at 12.659 kb on - strand, within GFF1160 at 12.727 kb on + strand, within GFF1160 at 12.727 kb on + strand, within GFF1160 at 12.744 kb on - strand, within GFF1160 at 12.785 kb on - strand, within GFF1160 at 12.924 kb on + strand at 12.928 kb on + strand at 12.934 kb on + strand at 13.234 kb on - strand, within GFF1162 at 13.257 kb on + strand, within GFF1162 at 13.257 kb on + strand, within GFF1162 at 13.257 kb on + strand, within GFF1162 at 13.379 kb on + strand, within GFF1162 at 13.379 kb on + strand, within GFF1162 at 13.380 kb on - strand, within GFF1162 at 13.576 kb on + strand, within GFF1162 at 13.576 kb on + strand, within GFF1162 at 13.594 kb on - strand, within GFF1162 at 13.597 kb on + strand, within GFF1162 at 13.597 kb on + strand, within GFF1162 at 13.598 kb on - strand, within GFF1162 at 13.684 kb on - strand at 13.825 kb on - strand, within GFF1163 at 13.858 kb on + strand, within GFF1163 at 14.019 kb on + strand at 14.055 kb on + strand at 14.151 kb on - strand, within GFF1164 at 14.224 kb on - strand, within GFF1164 at 14.245 kb on + strand, within GFF1164 at 14.246 kb on - strand, within GFF1164 at 14.247 kb on + strand, within GFF1164 at 14.298 kb on + strand, within GFF1164 at 14.401 kb on - strand, within GFF1164 at 14.554 kb on - strand, within GFF1165 at 14.564 kb on + strand, within GFF1165 at 14.564 kb on + strand, within GFF1165 at 14.564 kb on + strand, within GFF1165 at 14.565 kb on - strand, within GFF1165 at 14.605 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 12,220 + -0.4 12,220 + -2.7 12,242 + -0.3 12,243 - +0.7 12,305 + -0.6 12,332 - +0.2 12,342 + +0.4 12,384 + +0.9 12,385 - +0.0 12,385 - -0.3 12,424 + GFF1160 0.15 -0.2 12,659 - GFF1160 0.54 -0.6 12,727 + GFF1160 0.66 +0.1 12,727 + GFF1160 0.66 -0.4 12,744 - GFF1160 0.69 -0.1 12,785 - GFF1160 0.76 -1.3 12,924 + +0.4 12,928 + -0.1 12,934 + +0.5 13,234 - GFF1162 0.16 -0.9 13,257 + GFF1162 0.21 -0.2 13,257 + GFF1162 0.21 -0.9 13,257 + GFF1162 0.21 -0.8 13,379 + GFF1162 0.44 +0.1 13,379 + GFF1162 0.44 -0.3 13,380 - GFF1162 0.45 -0.4 13,576 + GFF1162 0.83 -0.0 13,576 + GFF1162 0.83 -0.4 13,594 - GFF1162 0.86 +0.1 13,597 + GFF1162 0.87 -0.1 13,597 + GFF1162 0.87 -0.2 13,598 - GFF1162 0.87 +0.3 13,684 - +0.2 13,825 - GFF1163 0.11 +0.3 13,858 + GFF1163 0.26 +0.2 14,019 + +0.2 14,055 + +0.2 14,151 - GFF1164 0.29 +0.4 14,224 - GFF1164 0.45 +0.7 14,245 + GFF1164 0.49 +0.3 14,246 - GFF1164 0.50 -0.2 14,247 + GFF1164 0.50 -0.2 14,298 + GFF1164 0.61 -0.5 14,401 - GFF1164 0.83 -0.2 14,554 - GFF1165 0.63 -0.1 14,564 + GFF1165 0.72 -0.1 14,564 + GFF1165 0.72 +0.3 14,564 + GFF1165 0.72 -0.3 14,565 - GFF1165 0.72 -0.2 14,605 + +0.7
Or see this region's nucleotide sequence