Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1144 and GFF1145 are separated by 42 nucleotides GFF1145 and GFF1146 overlap by 4 nucleotides GFF1146 and GFF1147 are separated by 34 nucleotides GFF1147 and GFF1148 are separated by 73 nucleotides GFF1148 and GFF1149 are separated by 156 nucleotides GFF1149 and GFF1150 are separated by 103 nucleotides
GFF1144 - FIG00732614: hypothetical protein, at 1,569 to 3,566
GFF1144
GFF1145 - PsiB protein, at 3,609 to 4,040
GFF1145
GFF1146 - PsiA protein, at 4,037 to 4,774
GFF1146
GFF1147 - hypothetical protein, at 4,809 to 4,937
GFF1147
GFF1148 - FIG01049080: hypothetical protein, at 5,011 to 5,181
GFF1148
GFF1149 - unnamed protein product, at 5,338 to 5,553
GFF1149
GFF1150 - unnamed protein product, at 5,657 to 5,890
GFF1150
Position (kb) 4
5 Strain fitness (log2 ratio) -1
0
1 at 3.216 kb on - strand, within GFF1144 at 3.502 kb on + strand at 3.502 kb on + strand at 3.503 kb on - strand at 3.503 kb on - strand at 3.568 kb on - strand at 3.621 kb on - strand at 3.641 kb on + strand at 3.641 kb on + strand at 3.641 kb on + strand at 3.641 kb on + strand at 3.855 kb on - strand, within GFF1145 at 3.881 kb on + strand, within GFF1145 at 3.881 kb on + strand, within GFF1145 at 3.945 kb on - strand, within GFF1145 at 3.953 kb on + strand, within GFF1145 at 3.953 kb on + strand, within GFF1145 at 3.991 kb on - strand, within GFF1145 at 4.093 kb on - strand at 4.220 kb on - strand, within GFF1146 at 4.220 kb on - strand, within GFF1146 at 4.313 kb on + strand, within GFF1146 at 4.314 kb on - strand, within GFF1146 at 4.368 kb on + strand, within GFF1146 at 4.369 kb on - strand, within GFF1146 at 4.502 kb on - strand, within GFF1146 at 4.536 kb on - strand, within GFF1146 at 4.538 kb on - strand, within GFF1146 at 4.607 kb on - strand, within GFF1146 at 4.667 kb on + strand, within GFF1146 at 4.703 kb on + strand at 4.703 kb on + strand at 4.703 kb on + strand at 4.703 kb on + strand at 4.703 kb on + strand at 4.703 kb on + strand at 4.704 kb on - strand at 4.704 kb on - strand at 4.704 kb on - strand at 4.704 kb on - strand at 4.889 kb on + strand, within GFF1147 at 4.889 kb on + strand, within GFF1147 at 4.889 kb on + strand, within GFF1147 at 4.889 kb on + strand, within GFF1147 at 4.889 kb on + strand, within GFF1147 at 4.890 kb on - strand, within GFF1147 at 4.890 kb on - strand, within GFF1147 at 4.890 kb on - strand, within GFF1147 at 4.990 kb on + strand at 5.039 kb on + strand, within GFF1148 at 5.111 kb on - strand, within GFF1148 at 5.130 kb on - strand, within GFF1148 at 5.130 kb on - strand, within GFF1148 at 5.130 kb on - strand, within GFF1148 at 5.267 kb on + strand at 5.269 kb on + strand at 5.271 kb on + strand at 5.421 kb on + strand, within GFF1149 at 5.504 kb on + strand, within GFF1149 at 5.529 kb on + strand, within GFF1149 at 5.618 kb on - strand at 5.667 kb on - strand at 5.689 kb on - strand, within GFF1150
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 3,216 - GFF1144 0.82 -0.1 3,502 + -0.8 3,502 + -0.5 3,503 - -0.2 3,503 - +0.3 3,568 - +0.7 3,621 - -0.9 3,641 + +0.2 3,641 + +0.0 3,641 + -0.9 3,641 + +0.5 3,855 - GFF1145 0.57 +0.0 3,881 + GFF1145 0.63 -0.3 3,881 + GFF1145 0.63 +0.2 3,945 - GFF1145 0.78 -0.1 3,953 + GFF1145 0.80 -0.9 3,953 + GFF1145 0.80 -0.2 3,991 - GFF1145 0.88 +0.5 4,093 - -0.7 4,220 - GFF1146 0.25 +0.3 4,220 - GFF1146 0.25 -1.3 4,313 + GFF1146 0.37 -0.1 4,314 - GFF1146 0.38 -0.7 4,368 + GFF1146 0.45 +0.2 4,369 - GFF1146 0.45 -0.6 4,502 - GFF1146 0.63 -0.8 4,536 - GFF1146 0.68 -0.3 4,538 - GFF1146 0.68 +1.0 4,607 - GFF1146 0.77 -0.9 4,667 + GFF1146 0.85 -0.4 4,703 + -0.8 4,703 + -0.4 4,703 + -0.0 4,703 + +0.2 4,703 + -0.8 4,703 + -0.8 4,704 - -0.8 4,704 - -0.8 4,704 - -0.5 4,704 - +0.2 4,889 + GFF1147 0.62 +1.0 4,889 + GFF1147 0.62 +1.0 4,889 + GFF1147 0.62 -0.3 4,889 + GFF1147 0.62 +0.0 4,889 + GFF1147 0.62 -0.1 4,890 - GFF1147 0.63 -0.6 4,890 - GFF1147 0.63 -1.3 4,890 - GFF1147 0.63 +0.2 4,990 + -0.7 5,039 + GFF1148 0.16 +0.5 5,111 - GFF1148 0.58 +0.3 5,130 - GFF1148 0.70 +0.4 5,130 - GFF1148 0.70 +0.1 5,130 - GFF1148 0.70 +0.1 5,267 + +0.3 5,269 + +0.2 5,271 + +0.3 5,421 + GFF1149 0.38 -0.8 5,504 + GFF1149 0.77 -0.7 5,529 + GFF1149 0.88 +0.2 5,618 - +0.4 5,667 - +0.5 5,689 - GFF1150 0.14 +0.0
Or see this region's nucleotide sequence