Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1142 and GFF1143 are separated by 54 nucleotides GFF1143 and GFF1144 are separated by 68 nucleotides
GFF1142 - Single-stranded DNA-binding protein, at 685 to 1,203
GFF1142
GFF1143 - FIG074102: hypothetical protein, at 1,258 to 1,500
GFF1143
GFF1144 - FIG00732614: hypothetical protein, at 1,569 to 3,566
GFF1144
Position (kb) 1
2 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 0.301 kb on + strand at 0.409 kb on + strand at 0.409 kb on + strand at 0.410 kb on - strand at 0.410 kb on - strand at 0.711 kb on + strand at 0.746 kb on + strand, within GFF1142 at 0.751 kb on + strand, within GFF1142 at 0.751 kb on + strand, within GFF1142 at 0.751 kb on + strand, within GFF1142 at 0.752 kb on - strand, within GFF1142 at 0.752 kb on - strand, within GFF1142 at 0.920 kb on - strand, within GFF1142 at 0.935 kb on + strand, within GFF1142 at 0.936 kb on - strand, within GFF1142 at 0.936 kb on - strand, within GFF1142 at 0.976 kb on + strand, within GFF1142 at 0.976 kb on + strand, within GFF1142 at 0.977 kb on - strand, within GFF1142 at 0.977 kb on - strand, within GFF1142 at 0.977 kb on - strand, within GFF1142 at 1.069 kb on - strand, within GFF1142 at 1.174 kb on + strand at 1.175 kb on - strand at 1.191 kb on + strand at 1.192 kb on - strand at 1.398 kb on + strand, within GFF1143 at 1.399 kb on - strand, within GFF1143 at 1.498 kb on + strand at 1.543 kb on - strand at 1.548 kb on + strand at 1.562 kb on - strand at 1.690 kb on + strand at 1.691 kb on - strand at 1.730 kb on + strand at 1.730 kb on + strand at 1.776 kb on - strand, within GFF1144 at 1.776 kb on - strand, within GFF1144 at 1.779 kb on - strand, within GFF1144 at 1.833 kb on + strand, within GFF1144 at 2.070 kb on + strand, within GFF1144 at 2.087 kb on - strand, within GFF1144 at 2.246 kb on + strand, within GFF1144 at 2.247 kb on - strand, within GFF1144 at 2.317 kb on - strand, within GFF1144 at 2.317 kb on - strand, within GFF1144 at 2.317 kb on - strand, within GFF1144 at 2.317 kb on - strand, within GFF1144 at 2.318 kb on + strand, within GFF1144 at 2.318 kb on + strand, within GFF1144 at 2.319 kb on - strand, within GFF1144 at 2.319 kb on - strand, within GFF1144 at 2.319 kb on - strand, within GFF1144
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 301 + -0.8 409 + +2.8 409 + +2.2 410 - +0.2 410 - +0.3 711 + +0.5 746 + GFF1142 0.12 +0.5 751 + GFF1142 0.13 +0.4 751 + GFF1142 0.13 -0.3 751 + GFF1142 0.13 +0.9 752 - GFF1142 0.13 +0.6 752 - GFF1142 0.13 -0.6 920 - GFF1142 0.45 -0.2 935 + GFF1142 0.48 +1.8 936 - GFF1142 0.48 -0.6 936 - GFF1142 0.48 +0.0 976 + GFF1142 0.56 +2.2 976 + GFF1142 0.56 +2.4 977 - GFF1142 0.56 -1.3 977 - GFF1142 0.56 +0.5 977 - GFF1142 0.56 -0.5 1,069 - GFF1142 0.74 +0.1 1,174 + +5.4 1,175 - +0.0 1,191 + +2.1 1,192 - -0.2 1,398 + GFF1143 0.58 +2.8 1,399 - GFF1143 0.58 +0.3 1,498 + +1.0 1,543 - -0.5 1,548 + +0.9 1,562 - +0.0 1,690 + -0.9 1,691 - +0.3 1,730 + -0.5 1,730 + +0.9 1,776 - GFF1144 0.10 -0.1 1,776 - GFF1144 0.10 -0.3 1,779 - GFF1144 0.11 -0.7 1,833 + GFF1144 0.13 +0.0 2,070 + GFF1144 0.25 -0.8 2,087 - GFF1144 0.26 +0.3 2,246 + GFF1144 0.34 -0.3 2,247 - GFF1144 0.34 +0.1 2,317 - GFF1144 0.37 -2.0 2,317 - GFF1144 0.37 -0.1 2,317 - GFF1144 0.37 +0.2 2,317 - GFF1144 0.37 -0.4 2,318 + GFF1144 0.37 +1.8 2,318 + GFF1144 0.37 +0.0 2,319 - GFF1144 0.38 +0.1 2,319 - GFF1144 0.38 +0.4 2,319 - GFF1144 0.38 +1.1
Or see this region's nucleotide sequence