Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1099 and GFF1100 are separated by 100 nucleotides GFF1100 and GFF1101 are separated by 4 nucleotides
GFF1099 - type 1 fimbriae major subunit FimA, at 285,253 to 285,795
GFF1099
GFF1100 - chaperone FimC, at 285,896 to 286,582
GFF1100
GFF1101 - type 1 fimbriae anchoring protein FimD, at 286,587 to 289,208
GFF1101
Position (kb)
285
286
287 Strain fitness (log2 ratio)
-1
0
1 at 285.047 kb on - strand at 285.266 kb on + strand at 285.354 kb on - strand, within GFF1099 at 285.623 kb on + strand, within GFF1099 at 285.624 kb on - strand, within GFF1099 at 285.887 kb on - strand at 285.890 kb on - strand at 286.166 kb on - strand, within GFF1100 at 286.177 kb on + strand, within GFF1100 at 286.306 kb on + strand, within GFF1100 at 286.312 kb on - strand, within GFF1100 at 286.419 kb on - strand, within GFF1100 at 286.439 kb on + strand, within GFF1100 at 286.439 kb on + strand, within GFF1100 at 286.469 kb on - strand, within GFF1100 at 286.587 kb on - strand at 286.699 kb on - strand at 286.786 kb on - strand at 286.800 kb on + strand at 286.812 kb on - strand at 286.925 kb on + strand, within GFF1101 at 287.052 kb on - strand, within GFF1101 at 287.091 kb on + strand, within GFF1101 at 287.092 kb on - strand, within GFF1101 at 287.092 kb on - strand, within GFF1101 at 287.219 kb on + strand, within GFF1101 at 287.319 kb on - strand, within GFF1101 at 287.322 kb on + strand, within GFF1101 at 287.373 kb on - strand, within GFF1101 at 287.373 kb on - strand, within GFF1101 at 287.376 kb on + strand, within GFF1101 at 287.376 kb on + strand, within GFF1101 at 287.376 kb on + strand, within GFF1101 at 287.376 kb on + strand, within GFF1101 at 287.377 kb on - strand, within GFF1101 at 287.379 kb on - strand, within GFF1101 at 287.420 kb on - strand, within GFF1101 at 287.441 kb on + strand, within GFF1101 at 287.441 kb on + strand, within GFF1101 at 287.442 kb on - strand, within GFF1101 at 287.442 kb on - strand, within GFF1101 at 287.530 kb on - strand, within GFF1101 at 287.570 kb on + strand, within GFF1101 at 287.571 kb on - strand, within GFF1101 at 287.573 kb on + strand, within GFF1101 at 287.574 kb on - strand, within GFF1101
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 285,047 - -0.0 285,266 + -0.1 285,354 - GFF1099 0.19 -0.6 285,623 + GFF1099 0.68 -0.1 285,624 - GFF1099 0.68 +0.4 285,887 - -0.3 285,890 - +0.3 286,166 - GFF1100 0.39 +0.6 286,177 + GFF1100 0.41 +0.6 286,306 + GFF1100 0.60 +0.7 286,312 - GFF1100 0.61 +0.1 286,419 - GFF1100 0.76 -0.2 286,439 + GFF1100 0.79 -0.2 286,439 + GFF1100 0.79 +0.3 286,469 - GFF1100 0.83 +0.4 286,587 - +0.6 286,699 - -0.6 286,786 - -1.2 286,800 + +0.5 286,812 - +0.4 286,925 + GFF1101 0.13 +0.2 287,052 - GFF1101 0.18 +1.1 287,091 + GFF1101 0.19 -0.1 287,092 - GFF1101 0.19 +0.3 287,092 - GFF1101 0.19 -0.4 287,219 + GFF1101 0.24 -0.0 287,319 - GFF1101 0.28 +1.4 287,322 + GFF1101 0.28 +0.9 287,373 - GFF1101 0.30 +0.1 287,373 - GFF1101 0.30 +0.2 287,376 + GFF1101 0.30 -0.5 287,376 + GFF1101 0.30 -0.6 287,376 + GFF1101 0.30 -0.0 287,376 + GFF1101 0.30 +0.2 287,377 - GFF1101 0.30 +0.5 287,379 - GFF1101 0.30 -0.6 287,420 - GFF1101 0.32 +0.5 287,441 + GFF1101 0.33 -0.3 287,441 + GFF1101 0.33 +0.5 287,442 - GFF1101 0.33 -0.2 287,442 - GFF1101 0.33 -0.2 287,530 - GFF1101 0.36 -0.2 287,570 + GFF1101 0.37 +0.7 287,571 - GFF1101 0.38 +0.2 287,573 + GFF1101 0.38 +0.8 287,574 - GFF1101 0.38 -0.1
Or see this region's nucleotide sequence