Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1077 and GFF1078 are separated by 360 nucleotides GFF1078 and GFF1079 are separated by 1 nucleotides GFF1079 and GFF1080 are separated by 3 nucleotides
GFF1077 - tRNA:Cm32/Um32 methyltransferase, at 260,074 to 260,760
GFF1077
GFF1078 - Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3), at 261,121 to 263,583
GFF1078
GFF1079 - Homoserine kinase (EC 2.7.1.39), at 263,585 to 264,514
GFF1079
GFF1080 - Threonine synthase (EC 4.2.3.1), at 264,518 to 265,804
GFF1080
Position (kb)
261
262
263
264 Strain fitness (log2 ratio)
-2
-1
0
1 at 260.364 kb on - strand, within GFF1077 at 260.364 kb on - strand, within GFF1077 at 260.366 kb on + strand, within GFF1077 at 260.524 kb on + strand, within GFF1077 at 260.525 kb on - strand, within GFF1077 at 260.525 kb on - strand, within GFF1077 at 260.525 kb on - strand, within GFF1077 at 260.525 kb on - strand, within GFF1077 at 260.913 kb on + strand at 260.933 kb on + strand at 260.933 kb on + strand at 260.933 kb on + strand at 260.933 kb on + strand at 260.962 kb on + strand at 261.045 kb on - strand at 261.113 kb on + strand at 261.310 kb on - strand at 261.441 kb on + strand, within GFF1078 at 261.598 kb on + strand, within GFF1078 at 261.818 kb on - strand, within GFF1078 at 261.818 kb on - strand, within GFF1078 at 261.819 kb on + strand, within GFF1078 at 261.820 kb on - strand, within GFF1078 at 261.820 kb on - strand, within GFF1078 at 261.871 kb on + strand, within GFF1078 at 261.871 kb on + strand, within GFF1078 at 262.050 kb on + strand, within GFF1078 at 262.050 kb on + strand, within GFF1078 at 262.392 kb on + strand, within GFF1078 at 262.393 kb on - strand, within GFF1078 at 262.393 kb on - strand, within GFF1078 at 262.613 kb on + strand, within GFF1078 at 262.783 kb on - strand, within GFF1078 at 262.810 kb on + strand, within GFF1078 at 262.883 kb on - strand, within GFF1078 at 262.886 kb on - strand, within GFF1078 at 263.164 kb on - strand, within GFF1078 at 263.274 kb on + strand, within GFF1078 at 263.340 kb on + strand at 263.340 kb on + strand at 263.345 kb on + strand at 263.346 kb on - strand at 263.471 kb on - strand at 263.473 kb on + strand at 263.474 kb on - strand at 263.597 kb on + strand at 263.597 kb on + strand at 264.124 kb on + strand, within GFF1079 at 264.472 kb on + strand at 264.472 kb on + strand at 264.473 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 260,364 - GFF1077 0.42 -0.3 260,364 - GFF1077 0.42 +0.1 260,366 + GFF1077 0.43 +1.3 260,524 + GFF1077 0.66 +0.1 260,525 - GFF1077 0.66 +0.1 260,525 - GFF1077 0.66 -0.5 260,525 - GFF1077 0.66 -0.2 260,525 - GFF1077 0.66 -1.0 260,913 + +0.2 260,933 + +0.6 260,933 + +0.6 260,933 + -0.0 260,933 + +0.3 260,962 + +0.9 261,045 - +0.2 261,113 + -0.3 261,310 - +0.4 261,441 + GFF1078 0.13 +0.3 261,598 + GFF1078 0.19 +0.0 261,818 - GFF1078 0.28 -0.6 261,818 - GFF1078 0.28 +0.0 261,819 + GFF1078 0.28 +1.4 261,820 - GFF1078 0.28 +0.4 261,820 - GFF1078 0.28 -2.3 261,871 + GFF1078 0.30 +0.2 261,871 + GFF1078 0.30 -0.2 262,050 + GFF1078 0.38 +0.3 262,050 + GFF1078 0.38 -0.2 262,392 + GFF1078 0.52 +0.0 262,393 - GFF1078 0.52 +0.3 262,393 - GFF1078 0.52 +0.4 262,613 + GFF1078 0.61 -0.2 262,783 - GFF1078 0.67 +0.3 262,810 + GFF1078 0.69 +0.0 262,883 - GFF1078 0.72 -0.3 262,886 - GFF1078 0.72 -0.1 263,164 - GFF1078 0.83 +0.5 263,274 + GFF1078 0.87 -0.9 263,340 + -0.1 263,340 + -0.2 263,345 + -1.0 263,346 - -0.3 263,471 - -0.2 263,473 + +0.1 263,474 - +0.1 263,597 + -0.6 263,597 + +0.3 264,124 + GFF1079 0.58 +0.4 264,472 + +0.7 264,472 + +0.2 264,473 - +0.8
Or see this region's nucleotide sequence