Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1078

Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1077 and GFF1078 are separated by 360 nucleotidesGFF1078 and GFF1079 are separated by 1 nucleotidesGFF1079 and GFF1080 are separated by 3 nucleotides GFF1077 - tRNA:Cm32/Um32 methyltransferase, at 260,074 to 260,760 GFF1077 GFF1078 - Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3), at 261,121 to 263,583 GFF1078 GFF1079 - Homoserine kinase (EC 2.7.1.39), at 263,585 to 264,514 GFF1079 GFF1080 - Threonine synthase (EC 4.2.3.1), at 264,518 to 265,804 GFF1080 Position (kb) 261 262 263 264Strain fitness (log2 ratio) -2 -1 0 1at 260.364 kb on - strand, within GFF1077at 260.364 kb on - strand, within GFF1077at 260.366 kb on + strand, within GFF1077at 260.524 kb on + strand, within GFF1077at 260.525 kb on - strand, within GFF1077at 260.525 kb on - strand, within GFF1077at 260.525 kb on - strand, within GFF1077at 260.525 kb on - strand, within GFF1077at 260.913 kb on + strandat 260.933 kb on + strandat 260.933 kb on + strandat 260.933 kb on + strandat 260.933 kb on + strandat 260.962 kb on + strandat 261.045 kb on - strandat 261.113 kb on + strandat 261.310 kb on - strandat 261.441 kb on + strand, within GFF1078at 261.598 kb on + strand, within GFF1078at 261.818 kb on - strand, within GFF1078at 261.818 kb on - strand, within GFF1078at 261.819 kb on + strand, within GFF1078at 261.820 kb on - strand, within GFF1078at 261.820 kb on - strand, within GFF1078at 261.871 kb on + strand, within GFF1078at 261.871 kb on + strand, within GFF1078at 262.050 kb on + strand, within GFF1078at 262.050 kb on + strand, within GFF1078at 262.392 kb on + strand, within GFF1078at 262.393 kb on - strand, within GFF1078at 262.393 kb on - strand, within GFF1078at 262.613 kb on + strand, within GFF1078at 262.783 kb on - strand, within GFF1078at 262.810 kb on + strand, within GFF1078at 262.883 kb on - strand, within GFF1078at 262.886 kb on - strand, within GFF1078at 263.164 kb on - strand, within GFF1078at 263.274 kb on + strand, within GFF1078at 263.340 kb on + strandat 263.340 kb on + strandat 263.345 kb on + strandat 263.346 kb on - strandat 263.471 kb on - strandat 263.473 kb on + strandat 263.474 kb on - strandat 263.597 kb on + strandat 263.597 kb on + strandat 264.124 kb on + strand, within GFF1079at 264.472 kb on + strandat 264.472 kb on + strandat 264.473 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI
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260,364 - GFF1077 0.42 -0.3
260,364 - GFF1077 0.42 +0.1
260,366 + GFF1077 0.43 +1.3
260,524 + GFF1077 0.66 +0.1
260,525 - GFF1077 0.66 +0.1
260,525 - GFF1077 0.66 -0.5
260,525 - GFF1077 0.66 -0.2
260,525 - GFF1077 0.66 -1.0
260,913 + +0.2
260,933 + +0.6
260,933 + +0.6
260,933 + -0.0
260,933 + +0.3
260,962 + +0.9
261,045 - +0.2
261,113 + -0.3
261,310 - +0.4
261,441 + GFF1078 0.13 +0.3
261,598 + GFF1078 0.19 +0.0
261,818 - GFF1078 0.28 -0.6
261,818 - GFF1078 0.28 +0.0
261,819 + GFF1078 0.28 +1.4
261,820 - GFF1078 0.28 +0.4
261,820 - GFF1078 0.28 -2.3
261,871 + GFF1078 0.30 +0.2
261,871 + GFF1078 0.30 -0.2
262,050 + GFF1078 0.38 +0.3
262,050 + GFF1078 0.38 -0.2
262,392 + GFF1078 0.52 +0.0
262,393 - GFF1078 0.52 +0.3
262,393 - GFF1078 0.52 +0.4
262,613 + GFF1078 0.61 -0.2
262,783 - GFF1078 0.67 +0.3
262,810 + GFF1078 0.69 +0.0
262,883 - GFF1078 0.72 -0.3
262,886 - GFF1078 0.72 -0.1
263,164 - GFF1078 0.83 +0.5
263,274 + GFF1078 0.87 -0.9
263,340 + -0.1
263,340 + -0.2
263,345 + -1.0
263,346 - -0.3
263,471 - -0.2
263,473 + +0.1
263,474 - +0.1
263,597 + -0.6
263,597 + +0.3
264,124 + GFF1079 0.58 +0.4
264,472 + +0.7
264,472 + +0.2
264,473 - +0.8

Or see this region's nucleotide sequence