Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1006 and GFF1007 are separated by 10 nucleotides GFF1007 and GFF1008 are separated by 94 nucleotides
GFF1006 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 189,652 to 190,608
GFF1006
GFF1007 - COG2879, Hypothetical small protein yjiX, at 190,619 to 190,822
GFF1007
GFF1008 - Carbon starvation protein A paralog, at 190,917 to 192,968
GFF1008
Position (kb) 190
191 Strain fitness (log2 ratio) -2
-1
0
1 at 189.715 kb on + strand at 189.961 kb on - strand, within GFF1006 at 190.051 kb on + strand, within GFF1006 at 190.177 kb on + strand, within GFF1006 at 190.248 kb on - strand, within GFF1006 at 190.259 kb on - strand, within GFF1006 at 190.375 kb on + strand, within GFF1006 at 190.376 kb on - strand, within GFF1006 at 190.379 kb on - strand, within GFF1006 at 190.547 kb on - strand at 190.581 kb on + strand at 190.586 kb on - strand at 190.609 kb on - strand at 190.609 kb on - strand at 190.656 kb on + strand, within GFF1007 at 190.692 kb on + strand, within GFF1007 at 190.693 kb on - strand, within GFF1007 at 190.693 kb on - strand, within GFF1007 at 190.693 kb on - strand, within GFF1007 at 190.701 kb on + strand, within GFF1007 at 190.701 kb on + strand, within GFF1007 at 190.701 kb on + strand, within GFF1007 at 190.702 kb on - strand, within GFF1007 at 190.702 kb on - strand, within GFF1007 at 190.702 kb on - strand, within GFF1007 at 190.740 kb on + strand, within GFF1007 at 190.741 kb on - strand, within GFF1007 at 190.741 kb on - strand, within GFF1007 at 190.741 kb on - strand, within GFF1007 at 190.741 kb on - strand, within GFF1007 at 190.813 kb on + strand at 190.877 kb on + strand at 191.033 kb on + strand at 191.034 kb on - strand at 191.034 kb on - strand at 191.037 kb on - strand at 191.041 kb on + strand at 191.063 kb on + strand at 191.083 kb on + strand at 191.083 kb on + strand at 191.159 kb on + strand, within GFF1008 at 191.177 kb on - strand, within GFF1008 at 191.282 kb on - strand, within GFF1008 at 191.352 kb on - strand, within GFF1008 at 191.422 kb on + strand, within GFF1008 at 191.422 kb on + strand, within GFF1008 at 191.651 kb on + strand, within GFF1008 at 191.691 kb on + strand, within GFF1008
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 189,715 + -0.3 189,961 - GFF1006 0.32 +0.3 190,051 + GFF1006 0.42 -0.1 190,177 + GFF1006 0.55 -0.2 190,248 - GFF1006 0.62 -0.2 190,259 - GFF1006 0.63 -0.1 190,375 + GFF1006 0.76 +0.2 190,376 - GFF1006 0.76 -0.2 190,379 - GFF1006 0.76 -1.2 190,547 - -0.2 190,581 + +0.4 190,586 - -0.2 190,609 - -0.3 190,609 - -0.3 190,656 + GFF1007 0.18 -0.1 190,692 + GFF1007 0.36 +0.1 190,693 - GFF1007 0.36 +0.1 190,693 - GFF1007 0.36 -1.8 190,693 - GFF1007 0.36 +0.0 190,701 + GFF1007 0.40 +0.3 190,701 + GFF1007 0.40 -0.7 190,701 + GFF1007 0.40 -0.3 190,702 - GFF1007 0.41 -1.0 190,702 - GFF1007 0.41 -0.7 190,702 - GFF1007 0.41 -0.5 190,740 + GFF1007 0.59 -1.2 190,741 - GFF1007 0.60 -0.4 190,741 - GFF1007 0.60 -0.1 190,741 - GFF1007 0.60 -0.7 190,741 - GFF1007 0.60 +0.3 190,813 + -0.2 190,877 + +0.4 191,033 + +0.7 191,034 - -0.7 191,034 - +0.2 191,037 - -0.0 191,041 + -0.3 191,063 + +0.0 191,083 + +0.5 191,083 + +0.6 191,159 + GFF1008 0.12 +0.1 191,177 - GFF1008 0.13 -1.7 191,282 - GFF1008 0.18 -0.2 191,352 - GFF1008 0.21 +0.1 191,422 + GFF1008 0.25 +1.0 191,422 + GFF1008 0.25 -0.9 191,651 + GFF1008 0.36 +0.5 191,691 + GFF1008 0.38 -0.2
Or see this region's nucleotide sequence