Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1002 and GFF1003 are separated by 197 nucleotides GFF1003 and GFF1004 are separated by 268 nucleotides GFF1004 and GFF1005 overlap by 14 nucleotides GFF1005 and GFF1006 are separated by 74 nucleotides
GFF1002 - Type I restriction-modification system, restriction subunit R (EC 3.1.21.3), at 184,111 to 187,620
GFF1002
GFF1003 - Mrr restriction system protein, at 187,818 to 188,732
GFF1003
GFF1004 - FIG01045296: hypothetical protein, at 189,001 to 189,288
GFF1004
GFF1005 - FIG00638610: hypothetical protein, at 189,275 to 189,577
GFF1005
GFF1006 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 189,652 to 190,608
GFF1006
Position (kb) 187
188
189 Strain fitness (log2 ratio) -1
0
1 at 186.830 kb on - strand, within GFF1002 at 186.950 kb on + strand, within GFF1002 at 186.951 kb on - strand, within GFF1002 at 187.527 kb on + strand at 187.528 kb on - strand at 187.571 kb on + strand at 187.789 kb on + strand at 187.790 kb on - strand at 187.795 kb on - strand at 187.834 kb on - strand at 188.186 kb on - strand, within GFF1003 at 188.344 kb on + strand, within GFF1003 at 188.344 kb on + strand, within GFF1003 at 188.345 kb on - strand, within GFF1003 at 188.432 kb on + strand, within GFF1003 at 188.432 kb on + strand, within GFF1003 at 188.524 kb on - strand, within GFF1003 at 188.524 kb on - strand, within GFF1003 at 188.602 kb on - strand, within GFF1003 at 188.602 kb on - strand, within GFF1003 at 188.607 kb on - strand, within GFF1003 at 188.666 kb on + strand at 188.672 kb on + strand at 188.680 kb on + strand at 188.681 kb on - strand at 189.037 kb on - strand, within GFF1004 at 189.195 kb on + strand, within GFF1004 at 189.231 kb on + strand, within GFF1004 at 189.516 kb on - strand, within GFF1005 at 189.715 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 186,830 - GFF1002 0.77 +0.5 186,950 + GFF1002 0.81 -0.1 186,951 - GFF1002 0.81 -0.0 187,527 + -0.6 187,528 - +0.1 187,571 + -0.6 187,789 + +0.4 187,790 - -0.0 187,795 - -0.5 187,834 - +0.1 188,186 - GFF1003 0.40 -0.9 188,344 + GFF1003 0.57 +0.4 188,344 + GFF1003 0.57 +0.1 188,345 - GFF1003 0.58 +0.6 188,432 + GFF1003 0.67 -0.6 188,432 + GFF1003 0.67 +0.1 188,524 - GFF1003 0.77 -1.0 188,524 - GFF1003 0.77 -0.4 188,602 - GFF1003 0.86 -0.5 188,602 - GFF1003 0.86 -1.5 188,607 - GFF1003 0.86 -0.5 188,666 + -0.6 188,672 + +0.4 188,680 + -0.3 188,681 - +0.3 189,037 - GFF1004 0.12 -1.1 189,195 + GFF1004 0.67 -0.2 189,231 + GFF1004 0.80 -0.5 189,516 - GFF1005 0.80 -0.3 189,715 + -0.3
Or see this region's nucleotide sequence