Experiment: D-Trehalose 20mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0181 and Echvi_0182 are separated by 49 nucleotides Echvi_0182 and Echvi_0183 overlap by 13 nucleotides Echvi_0183 and Echvi_0184 are separated by 54 nucleotides
Echvi_0181: Echvi_0181 - Phosphoglycerate dehydrogenase and related dehydrogenases, at 198,359 to 199,294
_0181
Echvi_0182: Echvi_0182 - Mg2+ transporter (mgtE), at 199,344 to 200,711
_0182
Echvi_0183: Echvi_0183 - dimethyladenosine transferase, at 200,699 to 201,481
_0183
Echvi_0184: Echvi_0184 - Leucyl aminopeptidase, at 201,536 to 202,972
_0184
Position (kb) 199
200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 199.065 kb on - strand, within Echvi_0181 at 199.256 kb on - strand at 199.288 kb on + strand at 199.289 kb on - strand at 199.332 kb on + strand at 199.332 kb on + strand at 199.333 kb on - strand at 199.333 kb on - strand at 200.756 kb on + strand at 200.756 kb on + strand at 200.757 kb on - strand at 200.821 kb on + strand, within Echvi_0183 at 200.854 kb on + strand, within Echvi_0183 at 200.946 kb on - strand, within Echvi_0183 at 200.991 kb on + strand, within Echvi_0183 at 200.991 kb on + strand, within Echvi_0183 at 200.996 kb on + strand, within Echvi_0183 at 200.997 kb on - strand, within Echvi_0183 at 200.997 kb on - strand, within Echvi_0183 at 201.036 kb on + strand, within Echvi_0183 at 201.036 kb on + strand, within Echvi_0183 at 201.036 kb on + strand, within Echvi_0183 at 201.037 kb on - strand, within Echvi_0183 at 201.157 kb on + strand, within Echvi_0183 at 201.182 kb on - strand, within Echvi_0183 at 201.183 kb on + strand, within Echvi_0183 at 201.184 kb on - strand, within Echvi_0183 at 201.239 kb on - strand, within Echvi_0183 at 201.249 kb on - strand, within Echvi_0183 at 201.371 kb on + strand, within Echvi_0183 at 201.485 kb on + strand at 201.503 kb on - strand at 201.533 kb on + strand at 201.533 kb on + strand at 201.533 kb on + strand at 201.533 kb on + strand at 201.534 kb on - strand at 201.535 kb on + strand at 201.535 kb on + strand at 201.535 kb on + strand at 201.535 kb on + strand at 201.535 kb on + strand at 201.536 kb on - strand at 201.536 kb on - strand at 201.549 kb on + strand at 201.558 kb on + strand at 201.558 kb on + strand at 201.601 kb on + strand at 201.601 kb on + strand at 201.601 kb on + strand at 201.602 kb on - strand at 201.630 kb on + strand at 201.636 kb on + strand at 201.636 kb on + strand at 201.640 kb on + strand at 201.640 kb on + strand at 201.640 kb on + strand at 201.641 kb on - strand at 201.643 kb on + strand at 201.662 kb on - strand at 201.672 kb on + strand at 201.672 kb on + strand at 201.685 kb on + strand, within Echvi_0184
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose 20mM (C) remove 199,065 - Echvi_0181 0.75 -2.8 199,256 - +1.4 199,288 + -0.6 199,289 - -3.1 199,332 + +0.7 199,332 + -0.8 199,333 - -0.1 199,333 - +0.8 200,756 + -0.1 200,756 + +1.5 200,757 - -0.1 200,821 + Echvi_0183 0.16 -0.2 200,854 + Echvi_0183 0.20 -0.5 200,946 - Echvi_0183 0.32 -0.1 200,991 + Echvi_0183 0.37 -1.9 200,991 + Echvi_0183 0.37 -1.0 200,996 + Echvi_0183 0.38 +0.4 200,997 - Echvi_0183 0.38 -0.4 200,997 - Echvi_0183 0.38 -0.9 201,036 + Echvi_0183 0.43 -1.4 201,036 + Echvi_0183 0.43 +0.4 201,036 + Echvi_0183 0.43 -1.0 201,037 - Echvi_0183 0.43 +0.9 201,157 + Echvi_0183 0.58 +3.7 201,182 - Echvi_0183 0.62 -0.6 201,183 + Echvi_0183 0.62 +0.8 201,184 - Echvi_0183 0.62 -0.7 201,239 - Echvi_0183 0.69 +0.0 201,249 - Echvi_0183 0.70 -2.2 201,371 + Echvi_0183 0.86 +0.1 201,485 + +0.3 201,503 - -2.8 201,533 + +0.2 201,533 + -0.2 201,533 + -0.3 201,533 + -0.5 201,534 - -1.2 201,535 + -0.9 201,535 + -2.4 201,535 + -1.5 201,535 + +0.0 201,535 + +0.7 201,536 - +0.9 201,536 - -1.8 201,549 + -0.6 201,558 + -2.2 201,558 + +1.4 201,601 + +4.6 201,601 + -0.3 201,601 + +2.2 201,602 - +3.1 201,630 + -0.7 201,636 + -1.2 201,636 + +0.8 201,640 + -1.6 201,640 + -2.7 201,640 + +1.1 201,641 - +0.3 201,643 + -1.3 201,662 - -0.8 201,672 + +0.5 201,672 + +3.5 201,685 + Echvi_0184 0.10 -1.5
Or see this region's nucleotide sequence