Experiment: KB with Nitrofurantoin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1408 and Psyr_1409 are separated by 85 nucleotides Psyr_1409 and Psyr_1410 are separated by 0 nucleotides Psyr_1410 and Psyr_1411 are separated by 134 nucleotides Psyr_1411 and Psyr_1412 overlap by 11 nucleotides
Psyr_1408: Psyr_1408 - Holliday junction endonuclease RuvC, at 1,594,950 to 1,595,474
_1408
Psyr_1409: Psyr_1409 - Holliday junction DNA helicase subunit RuvA, at 1,595,560 to 1,596,168
_1409
Psyr_1410: Psyr_1410 - Holliday junction DNA helicase RuvB, at 1,596,169 to 1,597,230
_1410
Psyr_1411: Psyr_1411 - 4-hydroxybenzoyl-CoA thioesterase, at 1,597,365 to 1,597,832
_1411
Psyr_1412: Psyr_1412 - Cell division and transport-associated protein TolQ, at 1,597,822 to 1,598,517
_1412
Position (kb)
1596
1597
1598 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1595.254 kb on - strand, within Psyr_1408 at 1595.405 kb on + strand, within Psyr_1408 at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.494 kb on - strand at 1595.500 kb on + strand at 1595.500 kb on + strand at 1595.501 kb on - strand at 1595.573 kb on + strand at 1595.855 kb on + strand, within Psyr_1409 at 1595.855 kb on + strand, within Psyr_1409 at 1595.864 kb on + strand, within Psyr_1409 at 1595.865 kb on - strand, within Psyr_1409 at 1596.167 kb on - strand at 1596.192 kb on - strand at 1596.426 kb on + strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.427 kb on - strand, within Psyr_1410 at 1596.551 kb on - strand, within Psyr_1410 at 1596.721 kb on + strand, within Psyr_1410 at 1597.082 kb on - strand, within Psyr_1410 at 1597.082 kb on - strand, within Psyr_1410 at 1597.099 kb on + strand, within Psyr_1410 at 1597.099 kb on + strand, within Psyr_1410 at 1597.100 kb on - strand, within Psyr_1410 at 1597.100 kb on - strand, within Psyr_1410 at 1597.101 kb on + strand, within Psyr_1410 at 1597.102 kb on - strand, within Psyr_1410 at 1597.165 kb on - strand at 1597.226 kb on + strand at 1597.226 kb on + strand at 1597.226 kb on + strand at 1597.226 kb on + strand at 1597.228 kb on + strand at 1597.228 kb on + strand at 1597.298 kb on + strand at 1597.298 kb on + strand at 1597.298 kb on + strand at 1597.298 kb on + strand at 1597.298 kb on + strand at 1597.298 kb on + strand at 1597.298 kb on + strand at 1597.298 kb on + strand at 1597.298 kb on + strand at 1597.298 kb on + strand at 1597.352 kb on + strand at 1597.352 kb on + strand at 1597.359 kb on + strand at 1597.364 kb on + strand at 1597.364 kb on + strand at 1597.446 kb on + strand, within Psyr_1411 at 1597.449 kb on + strand, within Psyr_1411 at 1597.449 kb on + strand, within Psyr_1411 at 1597.449 kb on + strand, within Psyr_1411 at 1597.449 kb on + strand, within Psyr_1411 at 1597.449 kb on + strand, within Psyr_1411 at 1597.458 kb on + strand, within Psyr_1411 at 1597.543 kb on + strand, within Psyr_1411 at 1597.652 kb on + strand, within Psyr_1411 at 1597.730 kb on + strand, within Psyr_1411 at 1597.736 kb on + strand, within Psyr_1411 at 1597.798 kb on + strand at 1597.798 kb on + strand at 1597.798 kb on + strand at 1597.798 kb on + strand at 1597.798 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 0.0625 mg/ml remove 1,595,254 - Psyr_1408 0.58 -2.5 1,595,405 + Psyr_1408 0.87 -0.3 1,595,481 + -0.9 1,595,481 + +1.1 1,595,481 + +0.1 1,595,481 + +0.2 1,595,481 + -0.1 1,595,481 + +0.9 1,595,481 + +1.3 1,595,482 - -0.9 1,595,482 - -3.8 1,595,482 - -2.9 1,595,482 - +0.7 1,595,482 - -1.9 1,595,482 - -1.9 1,595,482 - -1.3 1,595,483 + -0.1 1,595,483 + -0.4 1,595,483 + +0.3 1,595,483 + -1.1 1,595,483 + -1.3 1,595,483 + +0.9 1,595,483 + -0.4 1,595,483 + +0.3 1,595,483 + -0.1 1,595,483 + -0.8 1,595,483 + +1.0 1,595,483 + +0.2 1,595,483 + +0.4 1,595,483 + +1.9 1,595,483 + +0.5 1,595,484 - -1.7 1,595,484 - -0.2 1,595,484 - -1.3 1,595,484 - -3.7 1,595,484 - -1.7 1,595,484 - -0.3 1,595,484 - -1.5 1,595,484 - -0.6 1,595,494 - -1.2 1,595,500 + -0.4 1,595,500 + -1.7 1,595,501 - -0.0 1,595,573 + -2.9 1,595,855 + Psyr_1409 0.48 -1.3 1,595,855 + Psyr_1409 0.48 -1.7 1,595,864 + Psyr_1409 0.50 -0.3 1,595,865 - Psyr_1409 0.50 -0.9 1,596,167 - -1.5 1,596,192 - -1.9 1,596,426 + Psyr_1410 0.24 +0.7 1,596,427 - Psyr_1410 0.24 -2.1 1,596,427 - Psyr_1410 0.24 -3.0 1,596,427 - Psyr_1410 0.24 -1.6 1,596,427 - Psyr_1410 0.24 +0.1 1,596,427 - Psyr_1410 0.24 -3.0 1,596,551 - Psyr_1410 0.36 -1.6 1,596,721 + Psyr_1410 0.52 -1.9 1,597,082 - Psyr_1410 0.86 -1.3 1,597,082 - Psyr_1410 0.86 +0.7 1,597,099 + Psyr_1410 0.88 -0.6 1,597,099 + Psyr_1410 0.88 +0.4 1,597,100 - Psyr_1410 0.88 -0.3 1,597,100 - Psyr_1410 0.88 -0.5 1,597,101 + Psyr_1410 0.88 -2.9 1,597,102 - Psyr_1410 0.88 -2.6 1,597,165 - +0.8 1,597,226 + -0.4 1,597,226 + +2.3 1,597,226 + -0.2 1,597,226 + -0.9 1,597,228 + +0.3 1,597,228 + -0.9 1,597,298 + -0.6 1,597,298 + -0.0 1,597,298 + +0.7 1,597,298 + -0.7 1,597,298 + -1.3 1,597,298 + +0.9 1,597,298 + -0.5 1,597,298 + -1.3 1,597,298 + +0.2 1,597,298 + -1.3 1,597,352 + +0.2 1,597,352 + +0.0 1,597,359 + -0.1 1,597,364 + +0.0 1,597,364 + +0.4 1,597,446 + Psyr_1411 0.17 -0.4 1,597,449 + Psyr_1411 0.18 +0.1 1,597,449 + Psyr_1411 0.18 +0.4 1,597,449 + Psyr_1411 0.18 +1.9 1,597,449 + Psyr_1411 0.18 +0.3 1,597,449 + Psyr_1411 0.18 -0.5 1,597,458 + Psyr_1411 0.20 +1.4 1,597,543 + Psyr_1411 0.38 +0.2 1,597,652 + Psyr_1411 0.61 +1.0 1,597,730 + Psyr_1411 0.78 -0.2 1,597,736 + Psyr_1411 0.79 -0.7 1,597,798 + -0.4 1,597,798 + -0.3 1,597,798 + +0.1 1,597,798 + -0.3 1,597,798 + +1.1
Or see this region's nucleotide sequence