Experiment: 3-methyl-3-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhcH and yhcI overlap by 4 nucleotides yhcI and nanE overlap by 4 nucleotides nanE and nanT are separated by 47 nucleotides
b3221: yhcH - hypothetical protein (NCBI), at 3,367,036 to 3,367,500
yhcH
b3222: yhcI - putative NAGC-like transcriptional regulator (VIMSS), at 3,367,497 to 3,368,372
yhcI
b3223: nanE - predicted N-acetylmannosamine-6-P epimerase (NCBI), at 3,368,369 to 3,369,058
nanE
b3224: nanT - sialic acid transporter (VIMSS), at 3,369,106 to 3,370,596
nanT
Position (kb)
3368
3369
3370 Strain fitness (log2 ratio)
-2
-1
0
1 at 3367.386 kb on - strand, within yhcH at 3367.386 kb on - strand, within yhcH at 3367.444 kb on - strand, within yhcH at 3367.452 kb on - strand, within yhcH at 3367.454 kb on + strand at 3367.480 kb on - strand at 3367.480 kb on - strand at 3367.489 kb on + strand at 3367.523 kb on - strand at 3367.600 kb on + strand, within yhcI at 3367.600 kb on + strand, within yhcI at 3367.666 kb on - strand, within yhcI at 3367.678 kb on + strand, within yhcI at 3367.678 kb on + strand, within yhcI at 3367.691 kb on + strand, within yhcI at 3367.691 kb on + strand, within yhcI at 3367.725 kb on + strand, within yhcI at 3367.728 kb on + strand, within yhcI at 3367.768 kb on - strand, within yhcI at 3367.855 kb on - strand, within yhcI at 3367.855 kb on - strand, within yhcI at 3367.901 kb on + strand, within yhcI at 3368.002 kb on + strand, within yhcI at 3368.179 kb on - strand, within yhcI at 3368.179 kb on - strand, within yhcI at 3368.184 kb on - strand, within yhcI at 3368.285 kb on + strand at 3368.327 kb on - strand at 3368.368 kb on + strand at 3368.585 kb on + strand, within nanE at 3368.593 kb on - strand, within nanE at 3368.696 kb on - strand, within nanE at 3368.699 kb on + strand, within nanE at 3368.699 kb on + strand, within nanE at 3368.703 kb on + strand, within nanE at 3368.768 kb on + strand, within nanE at 3368.780 kb on + strand, within nanE at 3368.791 kb on + strand, within nanE at 3368.791 kb on + strand, within nanE at 3368.887 kb on + strand, within nanE at 3368.989 kb on - strand, within nanE at 3368.989 kb on - strand, within nanE at 3369.084 kb on + strand at 3369.297 kb on + strand, within nanT at 3369.480 kb on + strand, within nanT at 3369.488 kb on - strand, within nanT at 3369.488 kb on - strand, within nanT at 3369.538 kb on - strand, within nanT at 3369.640 kb on + strand, within nanT at 3369.669 kb on + strand, within nanT at 3369.791 kb on - strand, within nanT at 3369.793 kb on + strand, within nanT at 3369.831 kb on - strand, within nanT at 3369.941 kb on + strand, within nanT at 3370.043 kb on - strand, within nanT
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol 0.31 vol% remove 3,367,386 - yhcH b3221 0.75 -0.6 3,367,386 - yhcH b3221 0.75 +0.1 3,367,444 - yhcH b3221 0.88 -0.0 3,367,452 - yhcH b3221 0.89 +0.5 3,367,454 + +0.2 3,367,480 - +0.8 3,367,480 - +0.8 3,367,489 + -0.2 3,367,523 - +0.1 3,367,600 + yhcI b3222 0.12 -0.9 3,367,600 + yhcI b3222 0.12 -0.4 3,367,666 - yhcI b3222 0.19 +0.4 3,367,678 + yhcI b3222 0.21 +0.4 3,367,678 + yhcI b3222 0.21 +0.1 3,367,691 + yhcI b3222 0.22 +0.7 3,367,691 + yhcI b3222 0.22 -0.6 3,367,725 + yhcI b3222 0.26 -1.0 3,367,728 + yhcI b3222 0.26 +0.4 3,367,768 - yhcI b3222 0.31 +0.7 3,367,855 - yhcI b3222 0.41 +0.6 3,367,855 - yhcI b3222 0.41 +0.0 3,367,901 + yhcI b3222 0.46 +0.2 3,368,002 + yhcI b3222 0.58 -0.6 3,368,179 - yhcI b3222 0.78 -0.1 3,368,179 - yhcI b3222 0.78 +0.5 3,368,184 - yhcI b3222 0.78 -0.6 3,368,285 + +0.5 3,368,327 - +0.6 3,368,368 + -0.4 3,368,585 + nanE b3223 0.31 +0.3 3,368,593 - nanE b3223 0.32 -0.7 3,368,696 - nanE b3223 0.47 +0.1 3,368,699 + nanE b3223 0.48 -0.1 3,368,699 + nanE b3223 0.48 -0.0 3,368,703 + nanE b3223 0.48 +0.1 3,368,768 + nanE b3223 0.58 -0.5 3,368,780 + nanE b3223 0.60 -0.9 3,368,791 + nanE b3223 0.61 -0.3 3,368,791 + nanE b3223 0.61 +0.2 3,368,887 + nanE b3223 0.75 -0.1 3,368,989 - nanE b3223 0.90 +0.2 3,368,989 - nanE b3223 0.90 +0.9 3,369,084 + -0.3 3,369,297 + nanT b3224 0.13 +0.0 3,369,480 + nanT b3224 0.25 -1.8 3,369,488 - nanT b3224 0.26 +0.5 3,369,488 - nanT b3224 0.26 +0.3 3,369,538 - nanT b3224 0.29 +0.4 3,369,640 + nanT b3224 0.36 +0.3 3,369,669 + nanT b3224 0.38 +0.2 3,369,791 - nanT b3224 0.46 +0.3 3,369,793 + nanT b3224 0.46 +0.0 3,369,831 - nanT b3224 0.49 -0.2 3,369,941 + nanT b3224 0.56 +0.7 3,370,043 - nanT b3224 0.63 +0.4
Or see this region's nucleotide sequence