Experiment: 3-methyl-3-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hycE and hycD are separated by 17 nucleotides hycD and hycC are separated by 2 nucleotides hycC and hycB overlap by 4 nucleotides hycB and hycA are separated by 124 nucleotides
b2721: hycE - hydrogenase 3, large subunit (NCBI), at 2,842,784 to 2,844,493
hycE
b2722: hycD - hydrogenase 3, membrane subunit (NCBI), at 2,844,511 to 2,845,434
hycD
b2723: hycC - NADH dehydrogenase subunit N (NCBI), at 2,845,437 to 2,847,263
hycC
b2724: hycB - hydrogenase 3, Fe-S subunit (NCBI), at 2,847,260 to 2,847,871
hycB
b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457
hycA
Position (kb)
2845
2846
2847
2848 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2844.443 kb on - strand at 2844.462 kb on + strand at 2844.470 kb on - strand at 2844.470 kb on - strand at 2844.569 kb on - strand at 2844.576 kb on + strand at 2844.607 kb on - strand, within hycD at 2844.761 kb on - strand, within hycD at 2844.789 kb on - strand, within hycD at 2844.937 kb on + strand, within hycD at 2844.945 kb on - strand, within hycD at 2844.945 kb on - strand, within hycD at 2844.984 kb on + strand, within hycD at 2845.140 kb on - strand, within hycD at 2845.212 kb on - strand, within hycD at 2845.231 kb on - strand, within hycD at 2845.269 kb on - strand, within hycD at 2845.320 kb on + strand, within hycD at 2845.428 kb on - strand at 2845.428 kb on - strand at 2845.487 kb on + strand at 2845.594 kb on + strand at 2845.594 kb on + strand at 2846.220 kb on + strand, within hycC at 2846.226 kb on + strand, within hycC at 2846.264 kb on + strand, within hycC at 2846.269 kb on + strand, within hycC at 2846.269 kb on + strand, within hycC at 2846.269 kb on + strand, within hycC at 2846.269 kb on + strand, within hycC at 2846.272 kb on - strand, within hycC at 2846.345 kb on - strand, within hycC at 2846.381 kb on + strand, within hycC at 2846.406 kb on - strand, within hycC at 2846.406 kb on - strand, within hycC at 2846.475 kb on - strand, within hycC at 2846.506 kb on - strand, within hycC at 2846.699 kb on + strand, within hycC at 2846.699 kb on + strand, within hycC at 2846.731 kb on - strand, within hycC at 2846.810 kb on - strand, within hycC at 2846.886 kb on - strand, within hycC at 2846.886 kb on - strand, within hycC at 2846.941 kb on - strand, within hycC at 2846.953 kb on + strand, within hycC at 2846.953 kb on + strand, within hycC at 2846.977 kb on + strand, within hycC at 2846.985 kb on - strand, within hycC at 2846.985 kb on - strand, within hycC at 2847.072 kb on - strand, within hycC at 2847.099 kb on + strand at 2847.139 kb on - strand at 2847.276 kb on - strand at 2847.353 kb on + strand, within hycB at 2847.363 kb on + strand, within hycB at 2847.421 kb on + strand, within hycB at 2847.437 kb on - strand, within hycB at 2847.455 kb on + strand, within hycB at 2847.621 kb on + strand, within hycB at 2847.621 kb on + strand, within hycB at 2847.621 kb on - strand, within hycB at 2847.644 kb on + strand, within hycB at 2847.703 kb on + strand, within hycB at 2847.711 kb on - strand, within hycB at 2847.722 kb on - strand, within hycB at 2847.761 kb on - strand, within hycB at 2847.822 kb on - strand at 2847.829 kb on + strand at 2847.829 kb on + strand at 2847.888 kb on - strand at 2847.953 kb on - strand at 2848.071 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.082 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.253 kb on - strand, within hycA at 2848.253 kb on - strand, within hycA
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol 0.31 vol% remove 2,844,443 - +0.4 2,844,462 + -0.8 2,844,470 - -1.3 2,844,470 - -0.1 2,844,569 - -0.2 2,844,576 + -0.1 2,844,607 - hycD b2722 0.10 +0.5 2,844,761 - hycD b2722 0.27 +0.0 2,844,789 - hycD b2722 0.30 +0.5 2,844,937 + hycD b2722 0.46 +0.0 2,844,945 - hycD b2722 0.47 -0.3 2,844,945 - hycD b2722 0.47 +2.0 2,844,984 + hycD b2722 0.51 +0.6 2,845,140 - hycD b2722 0.68 +0.4 2,845,212 - hycD b2722 0.76 -0.8 2,845,231 - hycD b2722 0.78 -0.0 2,845,269 - hycD b2722 0.82 +0.7 2,845,320 + hycD b2722 0.88 +0.3 2,845,428 - +0.2 2,845,428 - +0.5 2,845,487 + -0.1 2,845,594 + -0.3 2,845,594 + -0.3 2,846,220 + hycC b2723 0.43 -0.4 2,846,226 + hycC b2723 0.43 +0.1 2,846,264 + hycC b2723 0.45 +1.0 2,846,269 + hycC b2723 0.46 -0.2 2,846,269 + hycC b2723 0.46 -1.6 2,846,269 + hycC b2723 0.46 +1.5 2,846,269 + hycC b2723 0.46 -0.6 2,846,272 - hycC b2723 0.46 -0.3 2,846,345 - hycC b2723 0.50 -0.4 2,846,381 + hycC b2723 0.52 +0.4 2,846,406 - hycC b2723 0.53 +0.2 2,846,406 - hycC b2723 0.53 +0.3 2,846,475 - hycC b2723 0.57 +0.2 2,846,506 - hycC b2723 0.59 +1.2 2,846,699 + hycC b2723 0.69 -0.1 2,846,699 + hycC b2723 0.69 -1.5 2,846,731 - hycC b2723 0.71 -0.7 2,846,810 - hycC b2723 0.75 +0.9 2,846,886 - hycC b2723 0.79 -2.7 2,846,886 - hycC b2723 0.79 -0.5 2,846,941 - hycC b2723 0.82 -0.1 2,846,953 + hycC b2723 0.83 -1.4 2,846,953 + hycC b2723 0.83 +0.1 2,846,977 + hycC b2723 0.84 -1.1 2,846,985 - hycC b2723 0.85 +1.2 2,846,985 - hycC b2723 0.85 +1.5 2,847,072 - hycC b2723 0.89 +0.3 2,847,099 + -0.2 2,847,139 - +0.4 2,847,276 - -0.0 2,847,353 + hycB b2724 0.15 -0.3 2,847,363 + hycB b2724 0.17 -0.4 2,847,421 + hycB b2724 0.26 -0.6 2,847,437 - hycB b2724 0.29 -0.7 2,847,455 + hycB b2724 0.32 +0.4 2,847,621 + hycB b2724 0.59 -0.3 2,847,621 + hycB b2724 0.59 +0.7 2,847,621 - hycB b2724 0.59 +0.0 2,847,644 + hycB b2724 0.63 +0.0 2,847,703 + hycB b2724 0.72 +0.9 2,847,711 - hycB b2724 0.74 -0.0 2,847,722 - hycB b2724 0.75 -0.7 2,847,761 - hycB b2724 0.82 -0.0 2,847,822 - -0.1 2,847,829 + -0.9 2,847,829 + -0.1 2,847,888 - -0.2 2,847,953 - +0.4 2,848,071 - hycA b2725 0.16 -1.4 2,848,073 - hycA b2725 0.17 +1.2 2,848,073 - hycA b2725 0.17 -0.5 2,848,082 - hycA b2725 0.19 -0.9 2,848,153 - hycA b2725 0.34 +0.4 2,848,153 - hycA b2725 0.34 -0.8 2,848,157 + hycA b2725 0.35 -0.4 2,848,157 + hycA b2725 0.35 +0.8 2,848,253 - hycA b2725 0.56 +0.3 2,848,253 - hycA b2725 0.56 -0.3
Or see this region's nucleotide sequence